The Effect of Luteolin and Luteoloside on the Secondary Structure of Lysozyme
The changes of lysozyme conformation in the absence and presence of luteolin and luteoloside were investigated by spectral analysis including fluorescence, UV, CD, Raman, and ATR-FTIR, and the biological activity of lysozyme was investigated by lysozyme assay kit. The results showed that the microen...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi-Wiley
2021-01-01
|
Series: | Journal of Food Quality |
Online Access: | http://dx.doi.org/10.1155/2021/5523965 |
id |
doaj-5ed7665827184754b4be049e853a96ad |
---|---|
record_format |
Article |
spelling |
doaj-5ed7665827184754b4be049e853a96ad2021-05-03T00:01:36ZengHindawi-WileyJournal of Food Quality1745-45572021-01-01202110.1155/2021/5523965The Effect of Luteolin and Luteoloside on the Secondary Structure of LysozymeYu Wu0Lijian Cui1Lingling Qu2Rong Wang3Ning Chen4Yun Huang5Yan Zhang6Pin Lv7Hebei Medical UniversityExperiment CenterHebei Medical UniversityHebei Medical UniversityHebei Medical UniversityHebei Medical UniversityHebei Medical UniversityHebei Medical UniversityThe changes of lysozyme conformation in the absence and presence of luteolin and luteoloside were investigated by spectral analysis including fluorescence, UV, CD, Raman, and ATR-FTIR, and the biological activity of lysozyme was investigated by lysozyme assay kit. The results showed that the microenvironment hydrophobicity of lysozyme increased and peptide extension decreased with the addition of luteolin or luteoloside. The α-helix of lysozyme might be influenced by luteolin or luteoloside, and its relative content had a significant difference after adding luteolin or luteoloside by the ATR-FTIR method, which was reconfirmed by CD and Raman spectra. The lysozyme activity changed obviously after adding luteolin or luteoloside. All of the conclusions above indicated the active site of lysozyme in the α-helix might be influenced by luteolin and luteoloside.http://dx.doi.org/10.1155/2021/5523965 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yu Wu Lijian Cui Lingling Qu Rong Wang Ning Chen Yun Huang Yan Zhang Pin Lv |
spellingShingle |
Yu Wu Lijian Cui Lingling Qu Rong Wang Ning Chen Yun Huang Yan Zhang Pin Lv The Effect of Luteolin and Luteoloside on the Secondary Structure of Lysozyme Journal of Food Quality |
author_facet |
Yu Wu Lijian Cui Lingling Qu Rong Wang Ning Chen Yun Huang Yan Zhang Pin Lv |
author_sort |
Yu Wu |
title |
The Effect of Luteolin and Luteoloside on the Secondary Structure of Lysozyme |
title_short |
The Effect of Luteolin and Luteoloside on the Secondary Structure of Lysozyme |
title_full |
The Effect of Luteolin and Luteoloside on the Secondary Structure of Lysozyme |
title_fullStr |
The Effect of Luteolin and Luteoloside on the Secondary Structure of Lysozyme |
title_full_unstemmed |
The Effect of Luteolin and Luteoloside on the Secondary Structure of Lysozyme |
title_sort |
effect of luteolin and luteoloside on the secondary structure of lysozyme |
publisher |
Hindawi-Wiley |
series |
Journal of Food Quality |
issn |
1745-4557 |
publishDate |
2021-01-01 |
description |
The changes of lysozyme conformation in the absence and presence of luteolin and luteoloside were investigated by spectral analysis including fluorescence, UV, CD, Raman, and ATR-FTIR, and the biological activity of lysozyme was investigated by lysozyme assay kit. The results showed that the microenvironment hydrophobicity of lysozyme increased and peptide extension decreased with the addition of luteolin or luteoloside. The α-helix of lysozyme might be influenced by luteolin or luteoloside, and its relative content had a significant difference after adding luteolin or luteoloside by the ATR-FTIR method, which was reconfirmed by CD and Raman spectra. The lysozyme activity changed obviously after adding luteolin or luteoloside. All of the conclusions above indicated the active site of lysozyme in the α-helix might be influenced by luteolin and luteoloside. |
url |
http://dx.doi.org/10.1155/2021/5523965 |
work_keys_str_mv |
AT yuwu theeffectofluteolinandluteolosideonthesecondarystructureoflysozyme AT lijiancui theeffectofluteolinandluteolosideonthesecondarystructureoflysozyme AT linglingqu theeffectofluteolinandluteolosideonthesecondarystructureoflysozyme AT rongwang theeffectofluteolinandluteolosideonthesecondarystructureoflysozyme AT ningchen theeffectofluteolinandluteolosideonthesecondarystructureoflysozyme AT yunhuang theeffectofluteolinandluteolosideonthesecondarystructureoflysozyme AT yanzhang theeffectofluteolinandluteolosideonthesecondarystructureoflysozyme AT pinlv theeffectofluteolinandluteolosideonthesecondarystructureoflysozyme AT yuwu effectofluteolinandluteolosideonthesecondarystructureoflysozyme AT lijiancui effectofluteolinandluteolosideonthesecondarystructureoflysozyme AT linglingqu effectofluteolinandluteolosideonthesecondarystructureoflysozyme AT rongwang effectofluteolinandluteolosideonthesecondarystructureoflysozyme AT ningchen effectofluteolinandluteolosideonthesecondarystructureoflysozyme AT yunhuang effectofluteolinandluteolosideonthesecondarystructureoflysozyme AT yanzhang effectofluteolinandluteolosideonthesecondarystructureoflysozyme AT pinlv effectofluteolinandluteolosideonthesecondarystructureoflysozyme |
_version_ |
1714634920377188352 |