Comparative in vitro study of the antimicrobial activity of metal-ZnO nanoparticles against several bacterial and fungal pathogens
Nowadays, the use of nanoparticles (NPs) has become useful in the different application fields. The aim of this study was to investigate the in vitro antimicrobial potential of metalZnO nanoparticles (ZnO NPs) against several bacterial and fungal strains including; Escherichia coli (ATCC 25922), B...
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Ain Shams University
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doaj-5edad80a2d6f466684aa4bccb9864bfb2021-08-19T20:38:43ZengAin Shams UniversityNovel Research in Microbiology Journal2537-02862537-02942021-08-01541338135010.21608/nrmj.2021.190251Comparative in vitro study of the antimicrobial activity of metal-ZnO nanoparticles against several bacterial and fungal pathogensMai A. Mwaheb0Laila R. Abd Al Halim1Tarob A. Abdel-Baset2Nada F. Hemeda3Department of botany, Faculty of science, Fayoum University, Fayoum 63514, EgyptAgricultural Microbiology Department, Faculty of Agriculture, Fayoum University, Fayoum 63514, EgyptDepartment of Physics, Faculty of Science, Fayoum University, Fayoum 63514, EgyptGenetics Department, Faculty of Agriculture, Fayoum University, Fayoum 63514, EgyptNowadays, the use of nanoparticles (NPs) has become useful in the different application fields. The aim of this study was to investigate the in vitro antimicrobial potential of metalZnO nanoparticles (ZnO NPs) against several bacterial and fungal strains including; Escherichia coli (ATCC 25922), Bacillus cereus (ATCC 13753), Staphylococcus aureus (ATCC 8095), Pseudomonas aeruginosa (ATCC10662), Candida albicans (ATCC10231) and Aspergillus niger (AUMC3663). Results obtained by X-ray diffraction analysis (XRD) showed that the NPs size was in the range of 35.1- 43.7 nm. Images of the scanning electron microscopy (SEM) demonstrated the rod shape nature of the ZnO NPs, and the semi-spherical shapes of the Zn9.7TM0.3O NPs. The effect of different concentrations of ZnO NPs on the in vitro growth of the bacterial and fungal strains was evaluated using the agar well diffusion assay. Current results showed that Cd-ZnO NP recorded the highest antimicrobial potency; expressing inhibition zones diameter range of 12- 45 mm, while ZnO NPs demonstrated the least activity exhibiting inhibition zones diameter that ranged from 0- 36 mm. Among all the examined ZnO-NPs, treatment of E. coli and Staph. aureus cells with Cd-ZnO proved to be the most effective in causing membrane leakage of reducing sugars, protein and DNA recording; 0.41 µg/ ml and 0.38 µg/ ml; 14.91 µg/ ml and 15.98 µg/ ml; 0.81 µg/ ml and 0.96 µg/ ml, respectively. This study emphasized that ZnO NPs could be used as alternative antimicrobial agents to control the bacterial and fungal pathogens. Manipulation of ZnO NPs is ecofriendly; as it reduces the use of the synthetic pesticides and chemical therapeutic agents, which pollute the environment. In the future, in vivo application of these NPs necessitates the proof that they have no phytotoxicity and\or cytotoxicity.https://nrmj.journals.ekb.eg/article_190251_b902d1e9df4cda18c030bef658a78370.pdfmetal-zno nanoparticlesantimicrobial activitymicrobial leakagereducing sugarsproteinsdna |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mai A. Mwaheb Laila R. Abd Al Halim Tarob A. Abdel-Baset Nada F. Hemeda |
spellingShingle |
Mai A. Mwaheb Laila R. Abd Al Halim Tarob A. Abdel-Baset Nada F. Hemeda Comparative in vitro study of the antimicrobial activity of metal-ZnO nanoparticles against several bacterial and fungal pathogens Novel Research in Microbiology Journal metal-zno nanoparticles antimicrobial activity microbial leakage reducing sugars proteins dna |
author_facet |
Mai A. Mwaheb Laila R. Abd Al Halim Tarob A. Abdel-Baset Nada F. Hemeda |
author_sort |
Mai A. Mwaheb |
title |
Comparative in vitro study of the antimicrobial activity of metal-ZnO nanoparticles against several bacterial and fungal pathogens |
title_short |
Comparative in vitro study of the antimicrobial activity of metal-ZnO nanoparticles against several bacterial and fungal pathogens |
title_full |
Comparative in vitro study of the antimicrobial activity of metal-ZnO nanoparticles against several bacterial and fungal pathogens |
title_fullStr |
Comparative in vitro study of the antimicrobial activity of metal-ZnO nanoparticles against several bacterial and fungal pathogens |
title_full_unstemmed |
Comparative in vitro study of the antimicrobial activity of metal-ZnO nanoparticles against several bacterial and fungal pathogens |
title_sort |
comparative in vitro study of the antimicrobial activity of metal-zno nanoparticles against several bacterial and fungal pathogens |
publisher |
Ain Shams University |
series |
Novel Research in Microbiology Journal |
issn |
2537-0286 2537-0294 |
publishDate |
2021-08-01 |
description |
Nowadays, the use of nanoparticles (NPs) has become useful in the different application
fields. The aim of this study was to investigate the in vitro antimicrobial potential of metalZnO nanoparticles (ZnO NPs) against several bacterial and fungal strains including;
Escherichia coli (ATCC 25922), Bacillus cereus (ATCC 13753), Staphylococcus aureus
(ATCC 8095), Pseudomonas aeruginosa (ATCC10662), Candida albicans (ATCC10231) and
Aspergillus niger (AUMC3663). Results obtained by X-ray diffraction analysis (XRD)
showed that the NPs size was in the range of 35.1- 43.7 nm. Images of the scanning electron
microscopy (SEM) demonstrated the rod shape nature of the ZnO NPs, and the semi-spherical
shapes of the Zn9.7TM0.3O NPs. The effect of different concentrations of ZnO NPs on the in
vitro growth of the bacterial and fungal strains was evaluated using the agar well diffusion
assay. Current results showed that Cd-ZnO NP recorded the highest antimicrobial potency;
expressing inhibition zones diameter range of 12- 45 mm, while ZnO NPs demonstrated the
least activity exhibiting inhibition zones diameter that ranged from 0- 36 mm. Among all the
examined ZnO-NPs, treatment of E. coli and Staph. aureus cells with Cd-ZnO proved to be
the most effective in causing membrane leakage of reducing sugars, protein and DNA
recording; 0.41 µg/ ml and 0.38 µg/ ml; 14.91 µg/ ml and 15.98 µg/ ml; 0.81 µg/ ml and 0.96
µg/ ml, respectively. This study emphasized that ZnO NPs could be used as alternative
antimicrobial agents to control the bacterial and fungal pathogens. Manipulation of ZnO NPs
is ecofriendly; as it reduces the use of the synthetic pesticides and chemical therapeutic agents,
which pollute the environment. In the future, in vivo application of these NPs necessitates the
proof that they have no phytotoxicity and\or cytotoxicity. |
topic |
metal-zno nanoparticles antimicrobial activity microbial leakage reducing sugars proteins dna |
url |
https://nrmj.journals.ekb.eg/article_190251_b902d1e9df4cda18c030bef658a78370.pdf |
work_keys_str_mv |
AT maiamwaheb comparativeinvitrostudyoftheantimicrobialactivityofmetalznonanoparticlesagainstseveralbacterialandfungalpathogens AT lailarabdalhalim comparativeinvitrostudyoftheantimicrobialactivityofmetalznonanoparticlesagainstseveralbacterialandfungalpathogens AT tarobaabdelbaset comparativeinvitrostudyoftheantimicrobialactivityofmetalznonanoparticlesagainstseveralbacterialandfungalpathogens AT nadafhemeda comparativeinvitrostudyoftheantimicrobialactivityofmetalznonanoparticlesagainstseveralbacterialandfungalpathogens |
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