Th17/Treg imbalance is associated with reduced indoleamine 2,3 dioxygenase activity in childhood allergic asthma
Abstract Background The differentiation of CD4+ lymphocytes Th17/regulatory T cells (Treg) and indoleamine 2,3-dioxygenase (IDO) is associated with the pathogenesis of allergic asthma. Basic research has shown that IDO is likely a “switch” of the transition from Th17 cells to Tregs under certain con...
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doaj-5edaf47cec77444a94aae517a407ed092020-11-25T03:28:59ZengBMCAllergy, Asthma & Clinical Immunology1710-14922020-07-0116111010.1186/s13223-020-00457-7Th17/Treg imbalance is associated with reduced indoleamine 2,3 dioxygenase activity in childhood allergic asthmaYing Hu0Zhiqiang Chen1Jing Zeng2Shouyan Zheng3Liujuan Sun4Li Zhu5Wei Liao6Department of Pediatrics, The First Affiliated Hospital of Army Medical UniversityDepartment of Pediatrics, The First Affiliated Hospital of Army Medical UniversityDepartment of Pediatrics, The First Affiliated Hospital of Army Medical UniversityDepartment of Pediatrics, The First Affiliated Hospital of Army Medical UniversityDepartment of Pediatrics, The First Affiliated Hospital of Army Medical UniversityDepartment of Pediatrics, The First Affiliated Hospital of Army Medical UniversityDepartment of Pediatrics, The First Affiliated Hospital of Army Medical UniversityAbstract Background The differentiation of CD4+ lymphocytes Th17/regulatory T cells (Treg) and indoleamine 2,3-dioxygenase (IDO) is associated with the pathogenesis of allergic asthma. Basic research has shown that IDO is likely a “switch” of the transition from Th17 cells to Tregs under certain conditions. However, no relevant clinical studies have been reported on the association between IDO activity and Th17/Treg imbalance in children with allergic asthma. The goal of this study was to test whether indoleamine 2,3 dioxygenase (IDO) participates in the pathogenesis of pediatric allergic asthma by influencing Th17/regulatory T cell (Treg) differentiation and related cytokines. Methods Thirty-three children with allergic asthma and 33 healthy children were selected. The subjects were evaluated via a pulmonary function test, a skin prick test, and an eosinophil count. Peripheral blood was collected to measure Th17/Treg percentages and related cytokine levels. Blood and induced sputum were obtained to measure the IDO level. Results Compared with the control group, the patient group had an obvious Th17/Treg imbalance; their IDO levels were significantly lower, their IL-17 and IL-6 levels were markedly higher, and their IL-10 and TGF-β levels were markedly lower than those of the control group. The IDO levels in both blood and induced sputum were negatively correlated with the Th17/Treg ratio. Conclusions A significant correlation was observed between IDO activity and Th17/Treg imbalance in children with allergic asthma. IDO may upregulate Treg numbers by stimulating IL-10 production and inhibiting IL-6 expression. Therefore, IDO may be a molecular switch that leads to the conversion of Th17 cells to Tregs, thus playing a potentially protective role in the pathogenesis of asthma. Trial registration This study was approved by the Chinese Clinical Trial Registry with registration number ChiCTR-COC-15006080 and was reviewed and approved by the Ethics Committee of Southwest Hospital. The name of registration: The effect of indoleamine 2,3 dioxygenase (IDO) on Regulation of Th17/Treg Differentiation in Childhood Asthma. Date of registration: 14/03/2015. URL of trial registry record: http://www.chictr.org.cnhttp://link.springer.com/article/10.1186/s13223-020-00457-7Th17/Treg imbalanceIDOChildhood allergic asthma |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ying Hu Zhiqiang Chen Jing Zeng Shouyan Zheng Liujuan Sun Li Zhu Wei Liao |
spellingShingle |
Ying Hu Zhiqiang Chen Jing Zeng Shouyan Zheng Liujuan Sun Li Zhu Wei Liao Th17/Treg imbalance is associated with reduced indoleamine 2,3 dioxygenase activity in childhood allergic asthma Allergy, Asthma & Clinical Immunology Th17/Treg imbalance IDO Childhood allergic asthma |
author_facet |
Ying Hu Zhiqiang Chen Jing Zeng Shouyan Zheng Liujuan Sun Li Zhu Wei Liao |
author_sort |
Ying Hu |
title |
Th17/Treg imbalance is associated with reduced indoleamine 2,3 dioxygenase activity in childhood allergic asthma |
title_short |
Th17/Treg imbalance is associated with reduced indoleamine 2,3 dioxygenase activity in childhood allergic asthma |
title_full |
Th17/Treg imbalance is associated with reduced indoleamine 2,3 dioxygenase activity in childhood allergic asthma |
title_fullStr |
Th17/Treg imbalance is associated with reduced indoleamine 2,3 dioxygenase activity in childhood allergic asthma |
title_full_unstemmed |
Th17/Treg imbalance is associated with reduced indoleamine 2,3 dioxygenase activity in childhood allergic asthma |
title_sort |
th17/treg imbalance is associated with reduced indoleamine 2,3 dioxygenase activity in childhood allergic asthma |
publisher |
BMC |
series |
Allergy, Asthma & Clinical Immunology |
issn |
1710-1492 |
publishDate |
2020-07-01 |
description |
Abstract Background The differentiation of CD4+ lymphocytes Th17/regulatory T cells (Treg) and indoleamine 2,3-dioxygenase (IDO) is associated with the pathogenesis of allergic asthma. Basic research has shown that IDO is likely a “switch” of the transition from Th17 cells to Tregs under certain conditions. However, no relevant clinical studies have been reported on the association between IDO activity and Th17/Treg imbalance in children with allergic asthma. The goal of this study was to test whether indoleamine 2,3 dioxygenase (IDO) participates in the pathogenesis of pediatric allergic asthma by influencing Th17/regulatory T cell (Treg) differentiation and related cytokines. Methods Thirty-three children with allergic asthma and 33 healthy children were selected. The subjects were evaluated via a pulmonary function test, a skin prick test, and an eosinophil count. Peripheral blood was collected to measure Th17/Treg percentages and related cytokine levels. Blood and induced sputum were obtained to measure the IDO level. Results Compared with the control group, the patient group had an obvious Th17/Treg imbalance; their IDO levels were significantly lower, their IL-17 and IL-6 levels were markedly higher, and their IL-10 and TGF-β levels were markedly lower than those of the control group. The IDO levels in both blood and induced sputum were negatively correlated with the Th17/Treg ratio. Conclusions A significant correlation was observed between IDO activity and Th17/Treg imbalance in children with allergic asthma. IDO may upregulate Treg numbers by stimulating IL-10 production and inhibiting IL-6 expression. Therefore, IDO may be a molecular switch that leads to the conversion of Th17 cells to Tregs, thus playing a potentially protective role in the pathogenesis of asthma. Trial registration This study was approved by the Chinese Clinical Trial Registry with registration number ChiCTR-COC-15006080 and was reviewed and approved by the Ethics Committee of Southwest Hospital. The name of registration: The effect of indoleamine 2,3 dioxygenase (IDO) on Regulation of Th17/Treg Differentiation in Childhood Asthma. Date of registration: 14/03/2015. URL of trial registry record: http://www.chictr.org.cn |
topic |
Th17/Treg imbalance IDO Childhood allergic asthma |
url |
http://link.springer.com/article/10.1186/s13223-020-00457-7 |
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