Identification of a Locus Controlling Compound Raceme Inflorescence in Mungbean [Vigna radiata (L.) R. Wilczek]
Mungbean [Vigna radiata (L.) R. Wilczek] produces a compound raceme inflorescence that branches into secondary inflorescences, which produce flowers. This architecture results in the less-domesticated traits of asynchronous pod maturity and multiple harvest times. This study identified the genetic f...
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doaj-5ee5ce113254460c95984eed7c3b26a22021-03-08T05:36:45ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-03-011210.3389/fgene.2021.642518642518Identification of a Locus Controlling Compound Raceme Inflorescence in Mungbean [Vigna radiata (L.) R. Wilczek]Eunsoo Lee0Xuefei Yang1Xuefei Yang2Jungmin Ha3Moon Young Kim4Moon Young Kim5Keum Yong Park6Keum Yong Park7Suk-Ha Lee8Suk-Ha Lee9Department of Agriculture, Forestry and Bioresources and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, South KoreaDepartment of Agriculture, Forestry and Bioresources and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, South KoreaPlant Genomics and Breeding Institute, Seoul National University, Seoul, South KoreaDepartment of Plant Science, Gangneung-Wonju National University, Gangneung, South KoreaDepartment of Agriculture, Forestry and Bioresources and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, South KoreaPlant Genomics and Breeding Institute, Seoul National University, Seoul, South KoreaDepartment of Agriculture, Forestry and Bioresources and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, South KoreaPlant Genomics and Breeding Institute, Seoul National University, Seoul, South KoreaDepartment of Agriculture, Forestry and Bioresources and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, South KoreaPlant Genomics and Breeding Institute, Seoul National University, Seoul, South KoreaMungbean [Vigna radiata (L.) R. Wilczek] produces a compound raceme inflorescence that branches into secondary inflorescences, which produce flowers. This architecture results in the less-domesticated traits of asynchronous pod maturity and multiple harvest times. This study identified the genetic factors responsible for the compound raceme of mungbean, providing a unique biological opportunity to improve simultaneous flowering. Using a recombinant inbred line (RIL) population derived from VC1973A, an elite cultivar with a compound raceme type, and IT208075, a natural mutant with a simple raceme type, a single locus that determined the inflorescence type was identified based on 1:1 segregation ratio in the F8 generation, and designated Comraceme. Linkage map analysis showed Comraceme was located on chromosome 4 within a marker interval spanning 520 kb and containing 64 genes. RILs carrying heterozygous fragments around Comraceme produced compound racemes, indicating this form was dominant to the simple raceme type. Quantitative trait loci related to plant architecture and inflorescence have been identified in genomic regions of soybean syntenic to Comraceme. In IT208075, 15 genes were present as distinct variants not observed in other landrace varieties or wild mungbean. These genes included Vradi04g00002481, a development-related gene encoding a B3 transcriptional factor. The upstream region of Vradi04g00002481 differed between lines producing the simple and compound types of raceme. Expression of Vradi04g00002481 was significantly lower at the early vegetative stage and higher at the early reproductive stage, in IT208075 than in VC1973A. Vradi04g00002481 was therefore likely to determine inflorescence type in mungbean. Although further study is required to determine the functional mechanism, this finding provides valuable genetic information for understanding the architecture of the compound raceme in mungbean.https://www.frontiersin.org/articles/10.3389/fgene.2021.642518/fullB3 transcription factorcompound racemeinflorescencelegumemungbeansynchronous maturity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eunsoo Lee Xuefei Yang Xuefei Yang Jungmin Ha Moon Young Kim Moon Young Kim Keum Yong Park Keum Yong Park Suk-Ha Lee Suk-Ha Lee |
spellingShingle |
Eunsoo Lee Xuefei Yang Xuefei Yang Jungmin Ha Moon Young Kim Moon Young Kim Keum Yong Park Keum Yong Park Suk-Ha Lee Suk-Ha Lee Identification of a Locus Controlling Compound Raceme Inflorescence in Mungbean [Vigna radiata (L.) R. Wilczek] Frontiers in Genetics B3 transcription factor compound raceme inflorescence legume mungbean synchronous maturity |
author_facet |
Eunsoo Lee Xuefei Yang Xuefei Yang Jungmin Ha Moon Young Kim Moon Young Kim Keum Yong Park Keum Yong Park Suk-Ha Lee Suk-Ha Lee |
author_sort |
Eunsoo Lee |
title |
Identification of a Locus Controlling Compound Raceme Inflorescence in Mungbean [Vigna radiata (L.) R. Wilczek] |
title_short |
Identification of a Locus Controlling Compound Raceme Inflorescence in Mungbean [Vigna radiata (L.) R. Wilczek] |
title_full |
Identification of a Locus Controlling Compound Raceme Inflorescence in Mungbean [Vigna radiata (L.) R. Wilczek] |
title_fullStr |
Identification of a Locus Controlling Compound Raceme Inflorescence in Mungbean [Vigna radiata (L.) R. Wilczek] |
title_full_unstemmed |
Identification of a Locus Controlling Compound Raceme Inflorescence in Mungbean [Vigna radiata (L.) R. Wilczek] |
title_sort |
identification of a locus controlling compound raceme inflorescence in mungbean [vigna radiata (l.) r. wilczek] |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Genetics |
issn |
1664-8021 |
publishDate |
2021-03-01 |
description |
Mungbean [Vigna radiata (L.) R. Wilczek] produces a compound raceme inflorescence that branches into secondary inflorescences, which produce flowers. This architecture results in the less-domesticated traits of asynchronous pod maturity and multiple harvest times. This study identified the genetic factors responsible for the compound raceme of mungbean, providing a unique biological opportunity to improve simultaneous flowering. Using a recombinant inbred line (RIL) population derived from VC1973A, an elite cultivar with a compound raceme type, and IT208075, a natural mutant with a simple raceme type, a single locus that determined the inflorescence type was identified based on 1:1 segregation ratio in the F8 generation, and designated Comraceme. Linkage map analysis showed Comraceme was located on chromosome 4 within a marker interval spanning 520 kb and containing 64 genes. RILs carrying heterozygous fragments around Comraceme produced compound racemes, indicating this form was dominant to the simple raceme type. Quantitative trait loci related to plant architecture and inflorescence have been identified in genomic regions of soybean syntenic to Comraceme. In IT208075, 15 genes were present as distinct variants not observed in other landrace varieties or wild mungbean. These genes included Vradi04g00002481, a development-related gene encoding a B3 transcriptional factor. The upstream region of Vradi04g00002481 differed between lines producing the simple and compound types of raceme. Expression of Vradi04g00002481 was significantly lower at the early vegetative stage and higher at the early reproductive stage, in IT208075 than in VC1973A. Vradi04g00002481 was therefore likely to determine inflorescence type in mungbean. Although further study is required to determine the functional mechanism, this finding provides valuable genetic information for understanding the architecture of the compound raceme in mungbean. |
topic |
B3 transcription factor compound raceme inflorescence legume mungbean synchronous maturity |
url |
https://www.frontiersin.org/articles/10.3389/fgene.2021.642518/full |
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