Comparison of the Active Compositions between Raw and Processed Epimedium from Different Species
Epimedium herb is one of the most vital traditional Chinese medicines (TCMs), which is used for “nourishing the kidney and reinforcing the Yang”. In the guidance of TCM theory, Epimedium herb is usually processed with lamb oil to increase its efficacy. The contents of active ingr...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2018-07-01
|
Series: | Molecules |
Subjects: | |
Online Access: | http://www.mdpi.com/1420-3049/23/7/1656 |
id |
doaj-5ee6cdf18242413c8e1be489297ccfcf |
---|---|
record_format |
Article |
spelling |
doaj-5ee6cdf18242413c8e1be489297ccfcf2020-11-25T00:10:00ZengMDPI AGMolecules1420-30492018-07-01237165610.3390/molecules23071656molecules23071656Comparison of the Active Compositions between Raw and Processed Epimedium from Different SpeciesHuamei Zhang0Hui Wang1Juan Wei2Xiaopeng Chen3Mengjie Sun4Huizi Ouyang5Jia Hao6Yanxu Chang7Zhiying Dou8Jun He9Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, ChinaEpimedium herb is one of the most vital traditional Chinese medicines (TCMs), which is used for “nourishing the kidney and reinforcing the Yang”. In the guidance of TCM theory, Epimedium herb is usually processed with lamb oil to increase its efficacy. The contents of active ingredients in different Epimedium are significantly varied, which may derive from their different species, regions and processing methods. In this research, 13 batches of raw Epimedium collected from 6 provinces were identified. After optimization of the processing method of Epimedium, a liquid chromatography–mass spectrometry (LC–MS/MS) method for simultaneous determination of 16 compounds was established to evaluate the quality of raw and processed. Then the multivariate statistical technique was applied to compare different batches of Epimedium based on the LC–MS/MS data. As a conclusion, the herbs collected from 6 areas were ascribed to 5 species by microscopic and appearance features. Meanwhile, all of the raw and processed samples were classified by partial least squares discriminant analysis (PLS-DA) based on the 16 analyzed compounds. The comparison results indicate that processing and species both have important influences on Epimedium compositions contents.http://www.mdpi.com/1420-3049/23/7/1656EpimediumidentificationprocessingLC–MS/MScomparison |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huamei Zhang Hui Wang Juan Wei Xiaopeng Chen Mengjie Sun Huizi Ouyang Jia Hao Yanxu Chang Zhiying Dou Jun He |
spellingShingle |
Huamei Zhang Hui Wang Juan Wei Xiaopeng Chen Mengjie Sun Huizi Ouyang Jia Hao Yanxu Chang Zhiying Dou Jun He Comparison of the Active Compositions between Raw and Processed Epimedium from Different Species Molecules Epimedium identification processing LC–MS/MS comparison |
author_facet |
Huamei Zhang Hui Wang Juan Wei Xiaopeng Chen Mengjie Sun Huizi Ouyang Jia Hao Yanxu Chang Zhiying Dou Jun He |
author_sort |
Huamei Zhang |
title |
Comparison of the Active Compositions between Raw and Processed Epimedium from Different Species |
title_short |
Comparison of the Active Compositions between Raw and Processed Epimedium from Different Species |
title_full |
Comparison of the Active Compositions between Raw and Processed Epimedium from Different Species |
title_fullStr |
Comparison of the Active Compositions between Raw and Processed Epimedium from Different Species |
title_full_unstemmed |
Comparison of the Active Compositions between Raw and Processed Epimedium from Different Species |
title_sort |
comparison of the active compositions between raw and processed epimedium from different species |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2018-07-01 |
description |
Epimedium herb is one of the most vital traditional Chinese medicines (TCMs), which is used for “nourishing the kidney and reinforcing the Yang”. In the guidance of TCM theory, Epimedium herb is usually processed with lamb oil to increase its efficacy. The contents of active ingredients in different Epimedium are significantly varied, which may derive from their different species, regions and processing methods. In this research, 13 batches of raw Epimedium collected from 6 provinces were identified. After optimization of the processing method of Epimedium, a liquid chromatography–mass spectrometry (LC–MS/MS) method for simultaneous determination of 16 compounds was established to evaluate the quality of raw and processed. Then the multivariate statistical technique was applied to compare different batches of Epimedium based on the LC–MS/MS data. As a conclusion, the herbs collected from 6 areas were ascribed to 5 species by microscopic and appearance features. Meanwhile, all of the raw and processed samples were classified by partial least squares discriminant analysis (PLS-DA) based on the 16 analyzed compounds. The comparison results indicate that processing and species both have important influences on Epimedium compositions contents. |
topic |
Epimedium identification processing LC–MS/MS comparison |
url |
http://www.mdpi.com/1420-3049/23/7/1656 |
work_keys_str_mv |
AT huameizhang comparisonoftheactivecompositionsbetweenrawandprocessedepimediumfromdifferentspecies AT huiwang comparisonoftheactivecompositionsbetweenrawandprocessedepimediumfromdifferentspecies AT juanwei comparisonoftheactivecompositionsbetweenrawandprocessedepimediumfromdifferentspecies AT xiaopengchen comparisonoftheactivecompositionsbetweenrawandprocessedepimediumfromdifferentspecies AT mengjiesun comparisonoftheactivecompositionsbetweenrawandprocessedepimediumfromdifferentspecies AT huiziouyang comparisonoftheactivecompositionsbetweenrawandprocessedepimediumfromdifferentspecies AT jiahao comparisonoftheactivecompositionsbetweenrawandprocessedepimediumfromdifferentspecies AT yanxuchang comparisonoftheactivecompositionsbetweenrawandprocessedepimediumfromdifferentspecies AT zhiyingdou comparisonoftheactivecompositionsbetweenrawandprocessedepimediumfromdifferentspecies AT junhe comparisonoftheactivecompositionsbetweenrawandprocessedepimediumfromdifferentspecies |
_version_ |
1725409738167943168 |