Altered activation state of circulating neutrophils in patients with neovascular age-related macular degeneration
Abstract Background Neutrophil dysfunction plays a key role in the development of diseases characterized by inflammation and angiogenesis. Here, we studied the systemic expression of neutrophil markers reflecting activation, adhesion, and resolution of inflammation in patients with neovascular age-r...
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doaj-5f007da0fc0945bea2bede6de6ce5d802020-11-24T21:03:02ZengBMCImmunity & Ageing1742-49332017-07-011411910.1186/s12979-017-0100-9Altered activation state of circulating neutrophils in patients with neovascular age-related macular degenerationMarie Krogh Nielsen0Sven Magnus Hector1Kelly Allen2Yousif Subhi3Torben Lykke Sørensen4Department of Ophthalmology, Zealand University HospitalDepartment of Ophthalmology, Zealand University HospitalDepartment of Ophthalmology, Zealand University HospitalDepartment of Ophthalmology, Zealand University HospitalDepartment of Ophthalmology, Zealand University HospitalAbstract Background Neutrophil dysfunction plays a key role in the development of diseases characterized by inflammation and angiogenesis. Here, we studied the systemic expression of neutrophil markers reflecting activation, adhesion, and resolution of inflammation in patients with neovascular age-related macular degeneration (AMD). Results This was a prospective case-control study of patients with neovascular AMD and age-matched healthy control individuals. Patients were recruited from an outpatient program, and control individuals were recruited amongst patients’ relatives. Current smokers and individuals with either active immune-disease or ongoing cancer were not included, as these factors are known to affect neutrophil function. Fresh-drawn venous blood was processed for flow cytometric analysis of neutrophil markers. We determined percentages of positive cells and compared expression levels using fluorescence intensity measures. We found conditional differences on marker expression between patients with neovascular AMD (n = 29) and controls (n = 28): no differences were found when looking broadly, but several differences emerged when focusing on non-smokers. Here, patients with neovascular AMD had increased expression of the activity marker cluster of differentiation (CD) 66b (P = 0.003; Mann-Whitney U test), decreased expression of adhesion marker CD162 (P = 0.044; Mann-Whitney U test), and lower expression of the resolution of inflammation marker C-X-C chemokine receptor 2 (P = 0.044; Mann-Whitney U test). Conclusions We present novel evidence suggesting that the activity of circulating neutrophils, sensitive to smoking, may differ in patients with neovascular AMD.http://link.springer.com/article/10.1186/s12979-017-0100-9NeutrophilsAge-related macular degenerationChoroidal neovascularizationFlow cytometryInflammation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marie Krogh Nielsen Sven Magnus Hector Kelly Allen Yousif Subhi Torben Lykke Sørensen |
spellingShingle |
Marie Krogh Nielsen Sven Magnus Hector Kelly Allen Yousif Subhi Torben Lykke Sørensen Altered activation state of circulating neutrophils in patients with neovascular age-related macular degeneration Immunity & Ageing Neutrophils Age-related macular degeneration Choroidal neovascularization Flow cytometry Inflammation |
author_facet |
Marie Krogh Nielsen Sven Magnus Hector Kelly Allen Yousif Subhi Torben Lykke Sørensen |
author_sort |
Marie Krogh Nielsen |
title |
Altered activation state of circulating neutrophils in patients with neovascular age-related macular degeneration |
title_short |
Altered activation state of circulating neutrophils in patients with neovascular age-related macular degeneration |
title_full |
Altered activation state of circulating neutrophils in patients with neovascular age-related macular degeneration |
title_fullStr |
Altered activation state of circulating neutrophils in patients with neovascular age-related macular degeneration |
title_full_unstemmed |
Altered activation state of circulating neutrophils in patients with neovascular age-related macular degeneration |
title_sort |
altered activation state of circulating neutrophils in patients with neovascular age-related macular degeneration |
publisher |
BMC |
series |
Immunity & Ageing |
issn |
1742-4933 |
publishDate |
2017-07-01 |
description |
Abstract Background Neutrophil dysfunction plays a key role in the development of diseases characterized by inflammation and angiogenesis. Here, we studied the systemic expression of neutrophil markers reflecting activation, adhesion, and resolution of inflammation in patients with neovascular age-related macular degeneration (AMD). Results This was a prospective case-control study of patients with neovascular AMD and age-matched healthy control individuals. Patients were recruited from an outpatient program, and control individuals were recruited amongst patients’ relatives. Current smokers and individuals with either active immune-disease or ongoing cancer were not included, as these factors are known to affect neutrophil function. Fresh-drawn venous blood was processed for flow cytometric analysis of neutrophil markers. We determined percentages of positive cells and compared expression levels using fluorescence intensity measures. We found conditional differences on marker expression between patients with neovascular AMD (n = 29) and controls (n = 28): no differences were found when looking broadly, but several differences emerged when focusing on non-smokers. Here, patients with neovascular AMD had increased expression of the activity marker cluster of differentiation (CD) 66b (P = 0.003; Mann-Whitney U test), decreased expression of adhesion marker CD162 (P = 0.044; Mann-Whitney U test), and lower expression of the resolution of inflammation marker C-X-C chemokine receptor 2 (P = 0.044; Mann-Whitney U test). Conclusions We present novel evidence suggesting that the activity of circulating neutrophils, sensitive to smoking, may differ in patients with neovascular AMD. |
topic |
Neutrophils Age-related macular degeneration Choroidal neovascularization Flow cytometry Inflammation |
url |
http://link.springer.com/article/10.1186/s12979-017-0100-9 |
work_keys_str_mv |
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1716774424194056192 |