Molecular characterization and immunohistochemical localization of a mitogen-activated protein kinase, Accp38b, from Apis cerana cerana
The p38 mitogen-activated protein kinase (MAPK) is involvedin various processes, including stress responses, development,and differentiation. However, little information on p38 MAPKin insects is available. In this study, a p38 MAPK gene,Accp38b, was isolated from Apis cerana cerana and characterized...
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Korean Society for Biochemistry and Molecular Biology
2012-05-01
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doaj-5f1d256637354707a8d8597802c71cf92020-11-25T00:59:00ZengKorean Society for Biochemistry and Molecular BiologyBMB Reports1976-66961976-670X2012-05-01455293298http://dx.doi.org/10.5483/BMBRep.2012.45.5.293Molecular characterization and immunohistochemical localization of a mitogen-activated protein kinase, Accp38b, from Apis cerana ceranaLiang ZhangFei MengYuzhen LiMingjiang KangXingqi GuoBaohua XuThe p38 mitogen-activated protein kinase (MAPK) is involvedin various processes, including stress responses, development,and differentiation. However, little information on p38 MAPKin insects is available. In this study, a p38 MAPK gene,Accp38b, was isolated from Apis cerana cerana and characterized.The quantitative real-time PCR (Q-PCR) analysis revealedthat Accp38b was induced by multiple stressors.Notably, the expression of Accp38b was relatively higher inthe pupae phase than in other developmental phases. Duringthe pupae phase, Accp38b expression was higher in the thoraxthan in the head and abdomen and higher in the fat body thanin the muscle and midgut. Immunohistochemisty showed significantpositive staining of Accp38b in sections from thebrain, eyes, fat body, and midgut of A. cerana cerana. Theseresults suggest that Accp38b may play a crucial role in stressresponses and have multiple aspects function during development.[BMB reports 2012; 45(5): 293-298]http://bmbreports.org/jbmb/pdf.php?data=MTMxMTA4MTVAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS01JTVEMTIwNTI0MjAyMV8lMjgyOTMtMjk4JTI5Qk1CXzExLTI1My5wZGY=Apis cerana ceranaExpression analysisImmunohistochemistryStress responsep38 MAPK |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Liang Zhang Fei Meng Yuzhen Li Mingjiang Kang Xingqi Guo Baohua Xu |
spellingShingle |
Liang Zhang Fei Meng Yuzhen Li Mingjiang Kang Xingqi Guo Baohua Xu Molecular characterization and immunohistochemical localization of a mitogen-activated protein kinase, Accp38b, from Apis cerana cerana BMB Reports Apis cerana cerana Expression analysis Immunohistochemistry Stress response p38 MAPK |
author_facet |
Liang Zhang Fei Meng Yuzhen Li Mingjiang Kang Xingqi Guo Baohua Xu |
author_sort |
Liang Zhang |
title |
Molecular characterization and immunohistochemical localization of a mitogen-activated protein kinase, Accp38b, from Apis cerana cerana |
title_short |
Molecular characterization and immunohistochemical localization of a mitogen-activated protein kinase, Accp38b, from Apis cerana cerana |
title_full |
Molecular characterization and immunohistochemical localization of a mitogen-activated protein kinase, Accp38b, from Apis cerana cerana |
title_fullStr |
Molecular characterization and immunohistochemical localization of a mitogen-activated protein kinase, Accp38b, from Apis cerana cerana |
title_full_unstemmed |
Molecular characterization and immunohistochemical localization of a mitogen-activated protein kinase, Accp38b, from Apis cerana cerana |
title_sort |
molecular characterization and immunohistochemical localization of a mitogen-activated protein kinase, accp38b, from apis cerana cerana |
publisher |
Korean Society for Biochemistry and Molecular Biology |
series |
BMB Reports |
issn |
1976-6696 1976-670X |
publishDate |
2012-05-01 |
description |
The p38 mitogen-activated protein kinase (MAPK) is involvedin various processes, including stress responses, development,and differentiation. However, little information on p38 MAPKin insects is available. In this study, a p38 MAPK gene,Accp38b, was isolated from Apis cerana cerana and characterized.The quantitative real-time PCR (Q-PCR) analysis revealedthat Accp38b was induced by multiple stressors.Notably, the expression of Accp38b was relatively higher inthe pupae phase than in other developmental phases. Duringthe pupae phase, Accp38b expression was higher in the thoraxthan in the head and abdomen and higher in the fat body thanin the muscle and midgut. Immunohistochemisty showed significantpositive staining of Accp38b in sections from thebrain, eyes, fat body, and midgut of A. cerana cerana. Theseresults suggest that Accp38b may play a crucial role in stressresponses and have multiple aspects function during development.[BMB reports 2012; 45(5): 293-298] |
topic |
Apis cerana cerana Expression analysis Immunohistochemistry Stress response p38 MAPK |
url |
http://bmbreports.org/jbmb/pdf.php?data=MTMxMTA4MTVAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS01JTVEMTIwNTI0MjAyMV8lMjgyOTMtMjk4JTI5Qk1CXzExLTI1My5wZGY= |
work_keys_str_mv |
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