Suppression of Melanose Caused by Diaporthe citri on Citrus Leaves Pretreated with Bio-sulfur
Melanose, caused by Diaporthe citri, is one of severe diseases in citrus, a major economic resource in Jeju island. To reduce the usage amount of organic synthetic fungicide, bio-sulfur was tested as an alternative chemical to control citrus melanose in the present study. Direct antifungal activity...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hanrimwon Publishing Company
2019-10-01
|
Series: | The Plant Pathology Journal |
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6788406 |
id |
doaj-5f33a0958df74b82b6a5464d59aeab70 |
---|---|
record_format |
Article |
spelling |
doaj-5f33a0958df74b82b6a5464d59aeab702020-11-25T01:49:34ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542019-10-0135541742410.5423/PPJ.OA.03.2019.006710.5423PPJ.OA.03.2019.0067Suppression of Melanose Caused by Diaporthe citri on Citrus Leaves Pretreated with Bio-sulfurYong Ho Shin0Eun Ju Ko1Su Jeong Kim2He Nam Hyun3Yong Chull Jeun4College of Applied Life Science, Major of Plant Resources & Environment, Jeju National University, Jeju 63243, KoreaCollege of Applied Life Science, Major of Plant Resources & Environment, Jeju National University, Jeju 63243, KoreaPostech Biotech Center, Pohang University of Science and Technology, Pohang 37673, KoreaCollege of Applied Life Science, Major of Plant Resources & Environment, Jeju National University, Jeju 63243, KoreaCollege of Applied Life Science, Major of Plant Resources & Environment, Jeju National University, Jeju 63243, KoreaMelanose, caused by Diaporthe citri, is one of severe diseases in citrus, a major economic resource in Jeju island. To reduce the usage amount of organic synthetic fungicide, bio-sulfur was tested as an alternative chemical to control citrus melanose in the present study. Direct antifungal activity of bio-sulfur against D. citri was determined through in vitro experiment using artificial nutrient media. Disease severity of melanose on bio-sulfur pretreated citrus leaves was lower than that on untreated ones. To illustrate the mechanism of disease suppression by bio-sulfur, infection structures were observed with a fluorescent microscope and a scanning electron microscope. In fluorescent microscopic observation, most conidia rarely germinated. In addition, hyphal growth on leaves pretreated with bio-sulfur was inhibited compared to that on untreated ones. In scanning electron microscope images of bio-sulfur pretreated leaves, surfaces of most conidia were shrunk while hyphae were morphologically changed and frequently branched. Such microscopic observations were also found for leaves pretreated with a commercial fungicide Dithianon. These results suggest that bio-sulfur may be used to control citrus melanose as an environment friendly alternative to organic synthetic fungicideshttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC6788406environment friendlyfluorescence microscopeinfection behaviorscanning electron microscope |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yong Ho Shin Eun Ju Ko Su Jeong Kim He Nam Hyun Yong Chull Jeun |
spellingShingle |
Yong Ho Shin Eun Ju Ko Su Jeong Kim He Nam Hyun Yong Chull Jeun Suppression of Melanose Caused by Diaporthe citri on Citrus Leaves Pretreated with Bio-sulfur The Plant Pathology Journal environment friendly fluorescence microscope infection behavior scanning electron microscope |
author_facet |
Yong Ho Shin Eun Ju Ko Su Jeong Kim He Nam Hyun Yong Chull Jeun |
author_sort |
Yong Ho Shin |
title |
Suppression of Melanose Caused by Diaporthe citri on Citrus Leaves Pretreated with Bio-sulfur |
title_short |
Suppression of Melanose Caused by Diaporthe citri on Citrus Leaves Pretreated with Bio-sulfur |
title_full |
Suppression of Melanose Caused by Diaporthe citri on Citrus Leaves Pretreated with Bio-sulfur |
title_fullStr |
Suppression of Melanose Caused by Diaporthe citri on Citrus Leaves Pretreated with Bio-sulfur |
title_full_unstemmed |
Suppression of Melanose Caused by Diaporthe citri on Citrus Leaves Pretreated with Bio-sulfur |
title_sort |
suppression of melanose caused by diaporthe citri on citrus leaves pretreated with bio-sulfur |
publisher |
Hanrimwon Publishing Company |
series |
The Plant Pathology Journal |
issn |
1598-2254 |
publishDate |
2019-10-01 |
description |
Melanose, caused by Diaporthe citri, is one of severe diseases in citrus, a major economic resource in Jeju island. To reduce the usage amount of organic synthetic fungicide, bio-sulfur was tested as an alternative chemical to control citrus melanose in the present study. Direct antifungal activity of bio-sulfur against D. citri was determined through in vitro experiment using artificial nutrient media. Disease severity of melanose on bio-sulfur pretreated citrus leaves was lower than that on untreated ones. To illustrate the mechanism of disease suppression by bio-sulfur, infection structures were observed with a fluorescent microscope and a scanning electron microscope. In fluorescent microscopic observation, most conidia rarely germinated. In addition, hyphal growth on leaves pretreated with bio-sulfur was inhibited compared to that on untreated ones. In scanning electron microscope images of bio-sulfur pretreated leaves, surfaces of most conidia were shrunk while hyphae were morphologically changed and frequently branched. Such microscopic observations were also found for leaves pretreated with a commercial fungicide Dithianon. These results suggest that bio-sulfur may be used to control citrus melanose as an environment friendly alternative to organic synthetic fungicides |
topic |
environment friendly fluorescence microscope infection behavior scanning electron microscope |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6788406 |
work_keys_str_mv |
AT yonghoshin suppressionofmelanosecausedbydiaporthecitrioncitrusleavespretreatedwithbiosulfur AT eunjuko suppressionofmelanosecausedbydiaporthecitrioncitrusleavespretreatedwithbiosulfur AT sujeongkim suppressionofmelanosecausedbydiaporthecitrioncitrusleavespretreatedwithbiosulfur AT henamhyun suppressionofmelanosecausedbydiaporthecitrioncitrusleavespretreatedwithbiosulfur AT yongchulljeun suppressionofmelanosecausedbydiaporthecitrioncitrusleavespretreatedwithbiosulfur |
_version_ |
1725006503348273152 |