The role of ubiquinone supplementation on osteogenesis of nonvascularized autogenous bone graft

• Main Authors: Irham Taufiqurrahman, Achmad Harijadi, Roberto M. Simanjuntak, Coen Pramono D, Istiati Istiati
• Format: Article
• Language: English
• Published: Universitas Airlangga 2015-03-01
• Series: Dental Journal: Majalah Kedokteran Gigi
• Subjects: Osteogenesis; ubiquinone; toxicity; hypoxia
• Online Access: http://e-journal.unair.ac.id/index.php/MKG/article/view/1673
• ISSN: 1978-3728; 2442-9740

<strong><em></em></strong><em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Ubiquinone is one of food supplement which is known have positive effect in wound healing. However the study </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>to evaluate the possible role of ubiquinone in bone healing in autogenous bone grafting after mandibular resection has not been </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>studied. An in vitro study is required to evaluate whether ubiquinone or coenzyme Q-10 (CoQ10) has a positive effect on osteogenesis. </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Viability test of CoQ10 and a model of osteogenic-induced and hypoxic-condition mesenchymal stem cell culture were established </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>to support the study. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Purpose: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The study was made to evaluate the role of ubiquinone in osteogenesis by analyzing the toxicity effect </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>and the optimal dose of CoQ10 that might interfere in bone marrow derived mesenchymal stem cell (BM-MSC) that was dose in cell </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>culture medium. The BM-MSC culture under hypoxia condition were also observed. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Method:</strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em> The toxicity and the optimum viability </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>concentration of ubiquinone were observed using MTT assay. The osteogenic differentiation under hypoxic condition was done on </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>BM-MSC in osteogenic medium that composed of ascorbic acid, glycerolphosphate and dexamethasone in hypoxia chamber for 21 </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>days. Osteogenic differentiation and cellular hypoxia features were tested with immunocytochemical staining using anti-Runx2 and </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>anti-HIF1</em><span style="font-family: SymbolMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">α <span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>monoclonal antibody, respectively. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Result: </strong><em>T</em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>he maximum density value of  1.826 was found in the group of ubiquinone </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>concentration of 75μM/ml, increasing of in concentration of ubiquinone resulted with the decrease ofoptical density of CoQ10. Statistic </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>analysis using Anova showed with no significant difference among groups with various concentration. Immunocytochemical staining </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>showed that Runx2 expression in 3% hypoxia group (p&lt;0.05). </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Conclusion:</strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Ubiquinone was found non toxic in its optimum dose of </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>75μM/ml, showed by optimum result in the expression ofRunx2 and HIF1</em><span style="font-family: SymbolMT; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;">α <span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>further study is necessary to evaluate the angiogenic and </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>osteogenic effect ofubiquione.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span><br style="font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /></span></span></span></span></span></span></span></span></span></span></span></span></span></span></em>