Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in Tobacco
Cadmium (Cd) is an environmental pollutant that potentially threatens human health worldwide. Developing approaches for efficiently treating environmental Cd is a priority. Selenium (Se) plays important role in the protection of plants against various abiotic stresses, including heavy metals. Previo...
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doaj-5f46b14feafb4def9db52869d1fb81fd2020-11-25T02:30:48ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-08-012015377210.3390/ijms20153772ijms20153772Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in TobaccoYong Luo0Yuewei Wei1Shuguang Sun2Jian Wang3Weifeng Wang4Dan Han5Huifang Shao6Hongfang Jia7Yunpeng Fu8National Tobacco Cultivation & Physiology & Biochemistry Research Centre, College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, ChinaNational Tobacco Cultivation & Physiology & Biochemistry Research Centre, College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, ChinaChina Tobacco Hubei Industrial Co., Ltd., Wuhan 430040, ChinaChina Tobacco Hubei Industrial Co., Ltd., Wuhan 430040, ChinaGuangxi Zhuang Autonomous Region Provincial Branch of China National Tobacco Corporation, Nanning 530000, ChinaNational Tobacco Cultivation & Physiology & Biochemistry Research Centre, College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, ChinaNational Tobacco Cultivation & Physiology & Biochemistry Research Centre, College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, ChinaNational Tobacco Cultivation & Physiology & Biochemistry Research Centre, College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, ChinaNational Tobacco Cultivation & Physiology & Biochemistry Research Centre, College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, ChinaCadmium (Cd) is an environmental pollutant that potentially threatens human health worldwide. Developing approaches for efficiently treating environmental Cd is a priority. Selenium (Se) plays important role in the protection of plants against various abiotic stresses, including heavy metals. Previous research has shown that Se can alleviate Cd toxicity, but the molecular mechanism is still not clear. In this study, we explore the function of auxin and phosphate (P) in tobacco (<i>Nicotiana tabacum</i>), with particular focus on their interaction with Se and Cd. Under Cd stress conditions, low Se (10 μM) significantly increased the biomass and antioxidant capacity of tobacco plants and reduced uptake of Cd. We also measured the auxin concentration and expression of auxin-relative genes in tobacco and found that plants treated with low Se (10 μM) had higher auxin concentrations at different Cd supply levels (0 μM, 20 μM, 50 μM) compared with no Se treatment, probably due to increased expression of auxin synthesis genes and auxin efflux carriers. Overexpression of a high affinity phosphate transporter NtPT2 enhanced the tolerance of tobacco to Cd stress, possibly by increasing the total P and Se content and decreasing Cd accumulation compared to that in the wild type (WT). Our results show that there is an interactive mechanism among P, Se, Cd, and auxin that affects plant growth and may provide a new approach for relieving Cd toxicity in plants.https://www.mdpi.com/1422-0067/20/15/3772seleniumcadmium stressauxinroot architecturephosphate transporter<i>Nicotiana tabacum</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yong Luo Yuewei Wei Shuguang Sun Jian Wang Weifeng Wang Dan Han Huifang Shao Hongfang Jia Yunpeng Fu |
spellingShingle |
Yong Luo Yuewei Wei Shuguang Sun Jian Wang Weifeng Wang Dan Han Huifang Shao Hongfang Jia Yunpeng Fu Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in Tobacco International Journal of Molecular Sciences selenium cadmium stress auxin root architecture phosphate transporter <i>Nicotiana tabacum</i> |
author_facet |
Yong Luo Yuewei Wei Shuguang Sun Jian Wang Weifeng Wang Dan Han Huifang Shao Hongfang Jia Yunpeng Fu |
author_sort |
Yong Luo |
title |
Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in Tobacco |
title_short |
Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in Tobacco |
title_full |
Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in Tobacco |
title_fullStr |
Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in Tobacco |
title_full_unstemmed |
Selenium Modulates the Level of Auxin to Alleviate the Toxicity of Cadmium in Tobacco |
title_sort |
selenium modulates the level of auxin to alleviate the toxicity of cadmium in tobacco |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2019-08-01 |
description |
Cadmium (Cd) is an environmental pollutant that potentially threatens human health worldwide. Developing approaches for efficiently treating environmental Cd is a priority. Selenium (Se) plays important role in the protection of plants against various abiotic stresses, including heavy metals. Previous research has shown that Se can alleviate Cd toxicity, but the molecular mechanism is still not clear. In this study, we explore the function of auxin and phosphate (P) in tobacco (<i>Nicotiana tabacum</i>), with particular focus on their interaction with Se and Cd. Under Cd stress conditions, low Se (10 μM) significantly increased the biomass and antioxidant capacity of tobacco plants and reduced uptake of Cd. We also measured the auxin concentration and expression of auxin-relative genes in tobacco and found that plants treated with low Se (10 μM) had higher auxin concentrations at different Cd supply levels (0 μM, 20 μM, 50 μM) compared with no Se treatment, probably due to increased expression of auxin synthesis genes and auxin efflux carriers. Overexpression of a high affinity phosphate transporter NtPT2 enhanced the tolerance of tobacco to Cd stress, possibly by increasing the total P and Se content and decreasing Cd accumulation compared to that in the wild type (WT). Our results show that there is an interactive mechanism among P, Se, Cd, and auxin that affects plant growth and may provide a new approach for relieving Cd toxicity in plants. |
topic |
selenium cadmium stress auxin root architecture phosphate transporter <i>Nicotiana tabacum</i> |
url |
https://www.mdpi.com/1422-0067/20/15/3772 |
work_keys_str_mv |
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