Is the poly (L- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration?
The purpose of this study was to compare: a new five-layered poly (L-lactide-co-caprolactone) (PLC) membrane and small intestinal submucosa (SIS) as a control in rat urinary bladder wall regeneration. The five-layered poly (L-lactide-co-caprolactone) membrane was prepared by an electrospinning proce...
Main Authors: | , , , , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014-01-01
|
Series: | PLoS ONE |
Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25162451/?tool=EBI |
id |
doaj-5f7532b108db4020b02a923b8eabad53 |
---|---|
record_format |
Article |
spelling |
doaj-5f7532b108db4020b02a923b8eabad532021-03-04T09:05:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0198e10529510.1371/journal.pone.0105295Is the poly (L- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration?Marta PokrywczynskaArkadiusz JundzillJan AdamowiczTomasz KowalczykKarolina WardaMarta RasmusLukasz BuchholzSandra KrzyzanowskaPawel NakielskiPawel NakielskiTomasz ChmielewskiMagdalena BodnarAndrzej MarszalekRobert DebskiMalgorzata Frontczak-BaniewiczGrzegorz MikułowskiMaciej NowackiTomasz A KowalewskiTomasz DrewaThe purpose of this study was to compare: a new five-layered poly (L-lactide-co-caprolactone) (PLC) membrane and small intestinal submucosa (SIS) as a control in rat urinary bladder wall regeneration. The five-layered poly (L-lactide-co-caprolactone) membrane was prepared by an electrospinning process. Adipose tissue was harvested from five 8-week old male Wistar rats. Adipose derived stem cells (ADSCs) were seeded in a density of 3×10(6) cells/cm2 onto PLC membrane and SIS scaffolds, and cultured for 5-7 days in the stem cell culture medium. Twenty male Wistar rats were randomly divided into five equal groups. Augmentation cystoplasty was performed in a previously created dome defect. Groups: (I) PLC+ 3×10(6)ADSCs; (II) SIS+ 3×10(6)ADSCs; (III) PLC; (IV) SIS; (V) control. Cystography was performed after three months. The reconstructed urinary bladders were evaluated in H&E and Masson's trichrome staining. Regeneration of all components of the normal urinary bladder wall was observed in bladders augmented with cell-seeded SIS matrices. The urinary bladders augmented with SIS matrices without cells showed fibrosis and graft contraction. Bladder augmentation with the PLC membrane led to numerous undesirable events including: bladder wall perforation, fistula or diverticula formation, and incorporation of the reconstructed wall into the bladder lumen. The new five-layered poly (L-lactide-co-caprolactone) membrane possesses poorer potential for regenerating the urinary bladder wall compared with SIS scaffold.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25162451/?tool=EBI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marta Pokrywczynska Arkadiusz Jundzill Jan Adamowicz Tomasz Kowalczyk Karolina Warda Marta Rasmus Lukasz Buchholz Sandra Krzyzanowska Pawel Nakielski Pawel Nakielski Tomasz Chmielewski Magdalena Bodnar Andrzej Marszalek Robert Debski Malgorzata Frontczak-Baniewicz Grzegorz Mikułowski Maciej Nowacki Tomasz A Kowalewski Tomasz Drewa |
spellingShingle |
Marta Pokrywczynska Arkadiusz Jundzill Jan Adamowicz Tomasz Kowalczyk Karolina Warda Marta Rasmus Lukasz Buchholz Sandra Krzyzanowska Pawel Nakielski Pawel Nakielski Tomasz Chmielewski Magdalena Bodnar Andrzej Marszalek Robert Debski Malgorzata Frontczak-Baniewicz Grzegorz Mikułowski Maciej Nowacki Tomasz A Kowalewski Tomasz Drewa Is the poly (L- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration? PLoS ONE |
author_facet |
Marta Pokrywczynska Arkadiusz Jundzill Jan Adamowicz Tomasz Kowalczyk Karolina Warda Marta Rasmus Lukasz Buchholz Sandra Krzyzanowska Pawel Nakielski Pawel Nakielski Tomasz Chmielewski Magdalena Bodnar Andrzej Marszalek Robert Debski Malgorzata Frontczak-Baniewicz Grzegorz Mikułowski Maciej Nowacki Tomasz A Kowalewski Tomasz Drewa |
author_sort |
Marta Pokrywczynska |
title |
Is the poly (L- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration? |
title_short |
Is the poly (L- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration? |
title_full |
Is the poly (L- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration? |
title_fullStr |
Is the poly (L- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration? |
title_full_unstemmed |
Is the poly (L- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration? |
title_sort |
is the poly (l- lactide- co- caprolactone) nanofibrous membrane suitable for urinary bladder regeneration? |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
The purpose of this study was to compare: a new five-layered poly (L-lactide-co-caprolactone) (PLC) membrane and small intestinal submucosa (SIS) as a control in rat urinary bladder wall regeneration. The five-layered poly (L-lactide-co-caprolactone) membrane was prepared by an electrospinning process. Adipose tissue was harvested from five 8-week old male Wistar rats. Adipose derived stem cells (ADSCs) were seeded in a density of 3×10(6) cells/cm2 onto PLC membrane and SIS scaffolds, and cultured for 5-7 days in the stem cell culture medium. Twenty male Wistar rats were randomly divided into five equal groups. Augmentation cystoplasty was performed in a previously created dome defect. Groups: (I) PLC+ 3×10(6)ADSCs; (II) SIS+ 3×10(6)ADSCs; (III) PLC; (IV) SIS; (V) control. Cystography was performed after three months. The reconstructed urinary bladders were evaluated in H&E and Masson's trichrome staining. Regeneration of all components of the normal urinary bladder wall was observed in bladders augmented with cell-seeded SIS matrices. The urinary bladders augmented with SIS matrices without cells showed fibrosis and graft contraction. Bladder augmentation with the PLC membrane led to numerous undesirable events including: bladder wall perforation, fistula or diverticula formation, and incorporation of the reconstructed wall into the bladder lumen. The new five-layered poly (L-lactide-co-caprolactone) membrane possesses poorer potential for regenerating the urinary bladder wall compared with SIS scaffold. |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25162451/?tool=EBI |
work_keys_str_mv |
AT martapokrywczynska isthepolyllactidecocaprolactonenanofibrousmembranesuitableforurinarybladderregeneration AT arkadiuszjundzill isthepolyllactidecocaprolactonenanofibrousmembranesuitableforurinarybladderregeneration AT janadamowicz isthepolyllactidecocaprolactonenanofibrousmembranesuitableforurinarybladderregeneration AT tomaszkowalczyk isthepolyllactidecocaprolactonenanofibrousmembranesuitableforurinarybladderregeneration AT karolinawarda isthepolyllactidecocaprolactonenanofibrousmembranesuitableforurinarybladderregeneration AT martarasmus isthepolyllactidecocaprolactonenanofibrousmembranesuitableforurinarybladderregeneration AT lukaszbuchholz isthepolyllactidecocaprolactonenanofibrousmembranesuitableforurinarybladderregeneration AT sandrakrzyzanowska isthepolyllactidecocaprolactonenanofibrousmembranesuitableforurinarybladderregeneration AT pawelnakielski isthepolyllactidecocaprolactonenanofibrousmembranesuitableforurinarybladderregeneration AT pawelnakielski isthepolyllactidecocaprolactonenanofibrousmembranesuitableforurinarybladderregeneration AT tomaszchmielewski isthepolyllactidecocaprolactonenanofibrousmembranesuitableforurinarybladderregeneration AT magdalenabodnar isthepolyllactidecocaprolactonenanofibrousmembranesuitableforurinarybladderregeneration AT andrzejmarszalek isthepolyllactidecocaprolactonenanofibrousmembranesuitableforurinarybladderregeneration AT robertdebski isthepolyllactidecocaprolactonenanofibrousmembranesuitableforurinarybladderregeneration AT malgorzatafrontczakbaniewicz isthepolyllactidecocaprolactonenanofibrousmembranesuitableforurinarybladderregeneration AT grzegorzmikułowski isthepolyllactidecocaprolactonenanofibrousmembranesuitableforurinarybladderregeneration AT maciejnowacki isthepolyllactidecocaprolactonenanofibrousmembranesuitableforurinarybladderregeneration AT tomaszakowalewski isthepolyllactidecocaprolactonenanofibrousmembranesuitableforurinarybladderregeneration AT tomaszdrewa isthepolyllactidecocaprolactonenanofibrousmembranesuitableforurinarybladderregeneration |
_version_ |
1714807432416329728 |