Development and Validation of a HPLC-UV Method for the Evaluation of Ellagic Acid in Liquid Extracts of Eugenia uniflora L. (Myrtaceae) Leaves and Its Ultrasound-Assisted Extraction Optimization
A simple HPLC-UV method has been developed and validated for the quantification of ellagic acid (EA) in ethanol extracts of Eugenia uniflora L. (Myrtaceae) leaves. The ultrasound-assisted extraction (UAE) optimization was performed using a Box Behnken design (33) combined with response surface metho...
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2017-01-01
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Online Access: | http://dx.doi.org/10.1155/2017/1501038 |
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doaj-5f8cb28254174f4c9ddc46b32cc13d632020-11-24T20:58:12ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882017-01-01201710.1155/2017/15010381501038Development and Validation of a HPLC-UV Method for the Evaluation of Ellagic Acid in Liquid Extracts of Eugenia uniflora L. (Myrtaceae) Leaves and Its Ultrasound-Assisted Extraction OptimizationPaulo Isaac Dias Assunção0Edemilson Cardoso da Conceição1Leonardo Luiz Borges2Joelma Abadia Marciano de Paula3Campus Anápolis de Ciências Exatas e Tecnológicas, Universidade Estadual de Goiás, Caixa Postal 459, 75132-903 Anápolis, GO, BrazilLaboratório de Pesquisa, Desenvolvimento & Inovação de Bioprodutos, Universidade Federal de Goiás, Caixa Postal 131, 74605-170 Goiânia, GO, BrazilCampus Anápolis de Ciências Exatas e Tecnológicas, Universidade Estadual de Goiás, Caixa Postal 459, 75132-903 Anápolis, GO, BrazilCampus Anápolis de Ciências Exatas e Tecnológicas, Universidade Estadual de Goiás, Caixa Postal 459, 75132-903 Anápolis, GO, BrazilA simple HPLC-UV method has been developed and validated for the quantification of ellagic acid (EA) in ethanol extracts of Eugenia uniflora L. (Myrtaceae) leaves. The ultrasound-assisted extraction (UAE) optimization was performed using a Box Behnken design (33) combined with response surface methodology to study the effects of the ethanol concentration (%, w/w), extraction time (minutes), and temperature (°C) on the EA concentration. The optimized results showed that the highest extraction yield of EA by UAE was 26.0 μg mL−1 when using 44% (w/w) ethanol as the solvent, 22 minutes as the extraction time, and 59°C as the extraction temperature. The concentration of EA in relation to the predicted value was 93.7% ± 0.4. UAE showed a strong potential for EA extraction.http://dx.doi.org/10.1155/2017/1501038 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Paulo Isaac Dias Assunção Edemilson Cardoso da Conceição Leonardo Luiz Borges Joelma Abadia Marciano de Paula |
spellingShingle |
Paulo Isaac Dias Assunção Edemilson Cardoso da Conceição Leonardo Luiz Borges Joelma Abadia Marciano de Paula Development and Validation of a HPLC-UV Method for the Evaluation of Ellagic Acid in Liquid Extracts of Eugenia uniflora L. (Myrtaceae) Leaves and Its Ultrasound-Assisted Extraction Optimization Evidence-Based Complementary and Alternative Medicine |
author_facet |
Paulo Isaac Dias Assunção Edemilson Cardoso da Conceição Leonardo Luiz Borges Joelma Abadia Marciano de Paula |
author_sort |
Paulo Isaac Dias Assunção |
title |
Development and Validation of a HPLC-UV Method for the Evaluation of Ellagic Acid in Liquid Extracts of Eugenia uniflora L. (Myrtaceae) Leaves and Its Ultrasound-Assisted Extraction Optimization |
title_short |
Development and Validation of a HPLC-UV Method for the Evaluation of Ellagic Acid in Liquid Extracts of Eugenia uniflora L. (Myrtaceae) Leaves and Its Ultrasound-Assisted Extraction Optimization |
title_full |
Development and Validation of a HPLC-UV Method for the Evaluation of Ellagic Acid in Liquid Extracts of Eugenia uniflora L. (Myrtaceae) Leaves and Its Ultrasound-Assisted Extraction Optimization |
title_fullStr |
Development and Validation of a HPLC-UV Method for the Evaluation of Ellagic Acid in Liquid Extracts of Eugenia uniflora L. (Myrtaceae) Leaves and Its Ultrasound-Assisted Extraction Optimization |
title_full_unstemmed |
Development and Validation of a HPLC-UV Method for the Evaluation of Ellagic Acid in Liquid Extracts of Eugenia uniflora L. (Myrtaceae) Leaves and Its Ultrasound-Assisted Extraction Optimization |
title_sort |
development and validation of a hplc-uv method for the evaluation of ellagic acid in liquid extracts of eugenia uniflora l. (myrtaceae) leaves and its ultrasound-assisted extraction optimization |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2017-01-01 |
description |
A simple HPLC-UV method has been developed and validated for the quantification of ellagic acid (EA) in ethanol extracts of Eugenia uniflora L. (Myrtaceae) leaves. The ultrasound-assisted extraction (UAE) optimization was performed using a Box Behnken design (33) combined with response surface methodology to study the effects of the ethanol concentration (%, w/w), extraction time (minutes), and temperature (°C) on the EA concentration. The optimized results showed that the highest extraction yield of EA by UAE was 26.0 μg mL−1 when using 44% (w/w) ethanol as the solvent, 22 minutes as the extraction time, and 59°C as the extraction temperature. The concentration of EA in relation to the predicted value was 93.7% ± 0.4. UAE showed a strong potential for EA extraction. |
url |
http://dx.doi.org/10.1155/2017/1501038 |
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