A Single-Step Purification and Molecular Characterization of Functional Shiga Toxin 2 Variants from Pathogenic <em>Escherichia coli</em>

A Single-Step Purification and Molecular Characterization of Functional Shiga Toxin 2 Variants from Pathogenic <em>Escherichia coli</em>

A one-step affinity chromatography method was developed to purify Shiga toxin 2 variants (Stx2) Stx2a, Stx2c, Stx2d and Stx2g from bacterial culture supernatants. Analysis of the purified Stx2 variants by denaturing gel electrophoresis revealed 32 kDa and 7 kDa protein bands, corresponding to the St...

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Bibliographic Details
Main Authors: Robert E. Mandrell, Stephanie McMahon, Beatriz Quiñones, Xiaohua He
Format: Article
Language:English
Published: MDPI AG 2012-06-01
Series:Toxins
Subjects:
cell-free translation assay
cytotoxicity
purification of Shiga toxins
Shiga toxin 2 variants
thermal stability of Shiga toxins
Online Access:http://www.mdpi.com/2072-6651/4/7/487

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http://www.mdpi.com/2072-6651/4/7/487

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