Generation of nonviral integration-free human iPS cell line KISCOi001-A from normal human fibroblasts, under defined xeno-free and feeder-free conditions

Generation of nonviral integration-free human iPS cell line KISCOi001-A from normal human fibroblasts, under defined xeno-free and feeder-free conditions

KISCOi001-A is a healthy feeder-free and fully characterized human induced pluripotent stem (iPS) cell line cultured under xeno-free and defined conditions. The cell line is generated from normal human foreskin fibroblasts with non-integrating episomal plasmid vectors encoding OCT4, SOX2, KLF4, NANO...

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Main Authors: Jose Inzunza, Jonathan Arias-Fuenzalida, Juan Segura-Aguilar, Ivan Nalvarte, Mukesh Varshney
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506121000398
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spelling doaj-5fafaa6bf96d49ac9d2a8ba7b16738412021-03-13T04:22:19ZengElsevierStem Cell Research1873-50612021-03-0151102193Generation of nonviral integration-free human iPS cell line KISCOi001-A from normal human fibroblasts, under defined xeno-free and feeder-free conditionsJose Inzunza0Jonathan Arias-Fuenzalida1Juan Segura-Aguilar2Ivan Nalvarte3Mukesh Varshney4Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, SwedenDepartment of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden; Centre for Cancer Cell Reprogramming, Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, NorwayMolecular and Clinical Pharmacology ICBM Faculty of Medicine, University of Chile, ChileDepartment of Biosciences and Nutrition, Karolinska Institutet, Huddinge, SwedenDepartment of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden; Corresponding author.KISCOi001-A is a healthy feeder-free and fully characterized human induced pluripotent stem (iPS) cell line cultured under xeno-free and defined conditions. The cell line is generated from normal human foreskin fibroblasts with non-integrating episomal plasmid vectors encoding OCT4, SOX2, KLF4, NANOG, LIN28, nontransforming L-MYC and dominant negative p53. The generated iPS cells are transgene-free and their pluripotency is confirmed by the expression of stem cell markers and capacity to differentiate into the cells of ectoderm, endoderm and mesoderm while their identity and karyotype stability is confirmed with Genomic assays.http://www.sciencedirect.com/science/article/pii/S1873506121000398
collection DOAJ
language English
format Article
sources DOAJ
author Jose Inzunza
Jonathan Arias-Fuenzalida
Juan Segura-Aguilar
Ivan Nalvarte
Mukesh Varshney
spellingShingle Jose Inzunza
Jonathan Arias-Fuenzalida
Juan Segura-Aguilar
Ivan Nalvarte
Mukesh Varshney
Generation of nonviral integration-free human iPS cell line KISCOi001-A from normal human fibroblasts, under defined xeno-free and feeder-free conditions
Stem Cell Research
author_facet Jose Inzunza
Jonathan Arias-Fuenzalida
Juan Segura-Aguilar
Ivan Nalvarte
Mukesh Varshney
author_sort Jose Inzunza
title Generation of nonviral integration-free human iPS cell line KISCOi001-A from normal human fibroblasts, under defined xeno-free and feeder-free conditions
title_short Generation of nonviral integration-free human iPS cell line KISCOi001-A from normal human fibroblasts, under defined xeno-free and feeder-free conditions
title_full Generation of nonviral integration-free human iPS cell line KISCOi001-A from normal human fibroblasts, under defined xeno-free and feeder-free conditions
title_fullStr Generation of nonviral integration-free human iPS cell line KISCOi001-A from normal human fibroblasts, under defined xeno-free and feeder-free conditions
title_full_unstemmed Generation of nonviral integration-free human iPS cell line KISCOi001-A from normal human fibroblasts, under defined xeno-free and feeder-free conditions
title_sort generation of nonviral integration-free human ips cell line kiscoi001-a from normal human fibroblasts, under defined xeno-free and feeder-free conditions
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2021-03-01
description KISCOi001-A is a healthy feeder-free and fully characterized human induced pluripotent stem (iPS) cell line cultured under xeno-free and defined conditions. The cell line is generated from normal human foreskin fibroblasts with non-integrating episomal plasmid vectors encoding OCT4, SOX2, KLF4, NANOG, LIN28, nontransforming L-MYC and dominant negative p53. The generated iPS cells are transgene-free and their pluripotency is confirmed by the expression of stem cell markers and capacity to differentiate into the cells of ectoderm, endoderm and mesoderm while their identity and karyotype stability is confirmed with Genomic assays.
url http://www.sciencedirect.com/science/article/pii/S1873506121000398
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