Effectiveness of a Controlled 5-FU Delivery Based on FZD10 Antibody-Conjugated Liposomes in Colorectal Cancer In vitro Models
The use of controlled delivery therapy in colorectal cancer (CRC) reduces toxicity and side effects. Recently, we have suggested that the Frizzled 10 (FZD10) protein, a cell surface receptor belonging to the FZD protein family that is overexpressed in CRC cells, is a novel candidate for targeting an...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-07-01
|
Series: | Pharmaceutics |
Subjects: | |
Online Access: | https://www.mdpi.com/1999-4923/12/7/650 |
id |
doaj-5fb8c106bd0e4b25907c231eae0f7029 |
---|---|
record_format |
Article |
spelling |
doaj-5fb8c106bd0e4b25907c231eae0f70292020-11-25T03:52:32ZengMDPI AGPharmaceutics1999-49232020-07-011265065010.3390/pharmaceutics12070650Effectiveness of a Controlled 5-FU Delivery Based on FZD10 Antibody-Conjugated Liposomes in Colorectal Cancer In vitro ModelsMaria Principia Scavo0Annalisa Cutrignelli1Nicoletta Depalo2Elisabetta Fanizza3Valentino Laquintana4Giampietro Gasparini5Gianluigi Giannelli6Nunzio Denora7Personalized Medicine Laboratory, National Institute of Gastroenterology “S. deBellis”, Via Turi 26 Castellana Grotte, 70125 Bari, ItalyDepartment of Pharmacy-Drug Science, University of Bari, Via E. Orabona 4, 70125 Bari, ItalyInstitute for Chemical and Physical Processes (IPCF)-CNR SS Bari, Via Orabona 4, 70125 Bari, ItalyInstitute for Chemical and Physical Processes (IPCF)-CNR SS Bari, Via Orabona 4, 70125 Bari, ItalyDepartment of Pharmacy-Drug Science, University of Bari, Via E. Orabona 4, 70125 Bari, ItalyOncology Unit, Hospital San Filippo Neri, 00135 Rome, ItalyScientific Direction, National Institute of Gastroenterology “de Bellis”, Via Turi 26 Castellana Grotte, 70125 Bari, ItalyDepartment of Pharmacy-Drug Science, University of Bari, Via E. Orabona 4, 70125 Bari, ItalyThe use of controlled delivery therapy in colorectal cancer (CRC) reduces toxicity and side effects. Recently, we have suggested that the Frizzled 10 (FZD10) protein, a cell surface receptor belonging to the FZD protein family that is overexpressed in CRC cells, is a novel candidate for targeting and treatment of CRC. Here, the anticancer effect of novel immuno-liposomes loaded with 5-Fluorouracil (5-FU), decorated with an antibody against FZD10 (anti-FZD10/5-FU/LPs), was evaluated in vitro on two different CRC cell lines, namely metastatic CoLo-205 and nonmetastatic CaCo-2 cells, that were found to overexpress FZD10. The anti-FZD10/5-FU/LPs obtained were extensively characterized and their preclinical therapeutic efficacy was evaluated with the MTS cell proliferation assay based on reduction of tetrazolium compound, scratch test, Field Emission Scanning Electron Microscopes (FE-SEM) investigation and immunofluorescence analysis. The results highlighted that the cytotoxic activity of 5-FU was enhanced when encapsulated in the anti-FZD10 /5-FU/LPs at the lowest tested concentrations, as compared to the free 5-FU counterparts. The immuno-liposomes proposed herein possess a great potential for selective treatment of CRC because, in future clinical applications, they can be encapsulated in gastro-resistant capsules or suppositories for oral or rectal delivery, thereby successfully reaching the intestinal tract in a minimally invasive manner.https://www.mdpi.com/1999-4923/12/7/650liposomestarget delivery nanosystemFZD10 proteincolon cancer therapy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maria Principia Scavo Annalisa Cutrignelli Nicoletta Depalo Elisabetta Fanizza Valentino Laquintana Giampietro Gasparini Gianluigi Giannelli Nunzio Denora |
spellingShingle |
Maria Principia Scavo Annalisa Cutrignelli Nicoletta Depalo Elisabetta Fanizza Valentino Laquintana Giampietro Gasparini Gianluigi Giannelli Nunzio Denora Effectiveness of a Controlled 5-FU Delivery Based on FZD10 Antibody-Conjugated Liposomes in Colorectal Cancer In vitro Models Pharmaceutics liposomes target delivery nanosystem FZD10 protein colon cancer therapy |
author_facet |
Maria Principia Scavo Annalisa Cutrignelli Nicoletta Depalo Elisabetta Fanizza Valentino Laquintana Giampietro Gasparini Gianluigi Giannelli Nunzio Denora |
author_sort |
Maria Principia Scavo |
title |
Effectiveness of a Controlled 5-FU Delivery Based on FZD10 Antibody-Conjugated Liposomes in Colorectal Cancer In vitro Models |
title_short |
Effectiveness of a Controlled 5-FU Delivery Based on FZD10 Antibody-Conjugated Liposomes in Colorectal Cancer In vitro Models |
title_full |
Effectiveness of a Controlled 5-FU Delivery Based on FZD10 Antibody-Conjugated Liposomes in Colorectal Cancer In vitro Models |
title_fullStr |
Effectiveness of a Controlled 5-FU Delivery Based on FZD10 Antibody-Conjugated Liposomes in Colorectal Cancer In vitro Models |
title_full_unstemmed |
Effectiveness of a Controlled 5-FU Delivery Based on FZD10 Antibody-Conjugated Liposomes in Colorectal Cancer In vitro Models |
title_sort |
effectiveness of a controlled 5-fu delivery based on fzd10 antibody-conjugated liposomes in colorectal cancer in vitro models |
publisher |
MDPI AG |
series |
Pharmaceutics |
issn |
1999-4923 |
publishDate |
2020-07-01 |
description |
The use of controlled delivery therapy in colorectal cancer (CRC) reduces toxicity and side effects. Recently, we have suggested that the Frizzled 10 (FZD10) protein, a cell surface receptor belonging to the FZD protein family that is overexpressed in CRC cells, is a novel candidate for targeting and treatment of CRC. Here, the anticancer effect of novel immuno-liposomes loaded with 5-Fluorouracil (5-FU), decorated with an antibody against FZD10 (anti-FZD10/5-FU/LPs), was evaluated in vitro on two different CRC cell lines, namely metastatic CoLo-205 and nonmetastatic CaCo-2 cells, that were found to overexpress FZD10. The anti-FZD10/5-FU/LPs obtained were extensively characterized and their preclinical therapeutic efficacy was evaluated with the MTS cell proliferation assay based on reduction of tetrazolium compound, scratch test, Field Emission Scanning Electron Microscopes (FE-SEM) investigation and immunofluorescence analysis. The results highlighted that the cytotoxic activity of 5-FU was enhanced when encapsulated in the anti-FZD10 /5-FU/LPs at the lowest tested concentrations, as compared to the free 5-FU counterparts. The immuno-liposomes proposed herein possess a great potential for selective treatment of CRC because, in future clinical applications, they can be encapsulated in gastro-resistant capsules or suppositories for oral or rectal delivery, thereby successfully reaching the intestinal tract in a minimally invasive manner. |
topic |
liposomes target delivery nanosystem FZD10 protein colon cancer therapy |
url |
https://www.mdpi.com/1999-4923/12/7/650 |
work_keys_str_mv |
AT mariaprincipiascavo effectivenessofacontrolled5fudeliverybasedonfzd10antibodyconjugatedliposomesincolorectalcancerinvitromodels AT annalisacutrignelli effectivenessofacontrolled5fudeliverybasedonfzd10antibodyconjugatedliposomesincolorectalcancerinvitromodels AT nicolettadepalo effectivenessofacontrolled5fudeliverybasedonfzd10antibodyconjugatedliposomesincolorectalcancerinvitromodels AT elisabettafanizza effectivenessofacontrolled5fudeliverybasedonfzd10antibodyconjugatedliposomesincolorectalcancerinvitromodels AT valentinolaquintana effectivenessofacontrolled5fudeliverybasedonfzd10antibodyconjugatedliposomesincolorectalcancerinvitromodels AT giampietrogasparini effectivenessofacontrolled5fudeliverybasedonfzd10antibodyconjugatedliposomesincolorectalcancerinvitromodels AT gianluigigiannelli effectivenessofacontrolled5fudeliverybasedonfzd10antibodyconjugatedliposomesincolorectalcancerinvitromodels AT nunziodenora effectivenessofacontrolled5fudeliverybasedonfzd10antibodyconjugatedliposomesincolorectalcancerinvitromodels |
_version_ |
1724482409244655616 |