Enzyme transient state kinetics in crystal and solution from the perspective of a time-resolved crystallographer

• Main Authors: Marius Schmidt, Dilano K. Saldin
• Format: Article
• Language: English
• Published: AIP Publishing LLC and ACA 2014-03-01
• Series: Structural Dynamics
• Online Access: http://dx.doi.org/10.1063/1.4869472

With recent technological advances at synchrotrons [Graber et al., J. Synchrotron Radiat. 18, 658–670 (2011)], it is feasible to rapidly collect time-resolved crystallographic data at multiple temperature settings [Schmidt et al., Acta Crystallogr. D 69, 2534–2542 (2013)], from which barriers of activation can be extracted. With the advent of fourth generation X-ray sources, new opportunities emerge to investigate structure and dynamics of biological macromolecules in real time [M. Schmidt, Adv. Condens. Matter Phys. 2013, 1–10] in crystals and potentially from single molecules in random orientation in solution [Poon et al., Adv. Condens. Matter Phys. 2013, 750371]. Kinetic data from time-resolved experiments on short time-scales must be interpreted in terms of chemical kinetics [Steinfeld et al., Chemical Kinetics and Dynamics, 2nd ed. (Prentience Hall, 1985)] and tied to existing time-resolved experiments on longer time-scales [Schmidt et al., Acta Crystallogr. D 69, 2534–2542 (2013); Jung et al., Nat. Chem. 5, 212–220 (2013)]. With this article, we will review and outline steps that are required to routinely determine the energetics of reactions in biomolecules in crystal and solution with newest X-ray sources. In eight sections, we aim to describe concepts and experimental details that may help to inspire new approaches to collect and interpret these data.