| Summary: | <p>Abstract</p> <p>Background</p> <p>Regulation of gene expression by microRNAs (miRNAs) plays a crucial role in many developmental and physiological processes in plants. miRNAs act to repress expression of their target genes via mRNA cleavage or translational repression. Dozens of miRNA families have been identified in rice, 21 of which are conserved between rice and Arabidopsis. miR172 is a conserved miRNA family which has been shown to regulate expression of <it>APETALA2 </it>(<it>AP2</it>)-like transcription factors in Arabidopsis and maize. The rice genome encodes five <it>AP2</it>-like genes predicted to be targets of miR172. To determine whether these rice <it>AP2</it>-like genes are regulated by miR172 and investigate the function of the target genes, we studied the effect of over-expressing two members of the miR172 family on rice plant development.</p> <p>Results</p> <p>Analysis of miR172 expression showed that it is most highly expressed in late vegetative stages and developing panicles. Analyses of expression of three miR172 targets showed that <it>SUPERNUMERARY BRACT </it>(<it>SNB</it>) and <it>Os03g60430 </it>have high expression in developing panicles. Expression of miR172 was not inversely correlated with expression of its targets although miR172-mediated cleavage of <it>SNB </it>was detected by 5' rapid amplification of cDNA ends (RACE). Over-expression of miR172b in rice delayed the transition from spikelet meristem to floral meristem, and resulted in floral and seed developmental defects, including changes to the number and identity of floral organs, lower fertility and reduced seed weight. Plants over-expressing miR172b not only phenocopied the T-DNA insertion mutant of <it>SNB </it>but showed additional defects in floret development not seen in the <it>snb </it>mutant. However <it>SNB </it>expression was not reduced in the miR172b over-expression plants.</p> <p>Conclusions</p> <p>The phenotypes resulting from over-expression of miR172b suggests it represses <it>SNB </it>and at least one of the other miR172 targets, most likely <it>Os03g60430</it>, indicating roles for other <it>AP2</it>-like genes in rice floret development. miR172 and the <it>AP2</it>-like genes had overlapping expression patterns in rice and their expression did not show an obvious negative correlation. There was not a uniform decrease in the expression of the <it>AP2</it>-like miR172 target mRNAs in the miR172b over-expression plants. These observations are consistent with miR172 functioning via translational repression or with expression of the <it>AP2</it>-like genes being regulated by a negative feedback loop.</p>
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