Human Endogenous Retroviruses Are Preferentially Expressed in Mononuclear Cells From Cord Blood Than From Maternal Blood and in the Fetal Part of Placenta
Background: Placenta shows high transcription levels of human endogenous retroviruses (HERVs) that are overexpressed during embryonic and fetal development.Methods: In order to gather further information on the degree of HERV activation in maternal and fetal tissues we assessed the transcription lev...
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doaj-5fc580c805ba48b5a441f6ab18f769832020-11-25T02:10:34ZengFrontiers Media S.A.Frontiers in Pediatrics2296-23602020-05-01810.3389/fped.2020.00244506865Human Endogenous Retroviruses Are Preferentially Expressed in Mononuclear Cells From Cord Blood Than From Maternal Blood and in the Fetal Part of PlacentaMassimiliano Bergallo0Massimiliano Bergallo1Luca Marozio2Giovanni Botta3Annalisa Tancredi4Valentina Daprà5Valentina Daprà6Ilaria Galliano7Ilaria Galliano8Paola Montanari9Paola Montanari10Alessandra Coscia11Chiara Benedetto12Pier Angelo Tovo13Pediatric Laboratory, Department of Pediatric Sciences and Public Health, University of Turin, Turin, ItalyDepartment of Pediatric Sciences and Public Health, University of Turin, Turin, ItalyDepartment of Surgical Sciences, Obstetrics and Gynecology 1, University of Turin, Turin, ItalyDepartment of Pathology AOU Città della Salute e della Scienza di Torino, Turin, ItalyDepartment of Surgical Sciences, Obstetrics and Gynecology 1, University of Turin, Turin, ItalyPediatric Laboratory, Department of Pediatric Sciences and Public Health, University of Turin, Turin, ItalyDepartment of Pediatric Sciences and Public Health, University of Turin, Turin, ItalyPediatric Laboratory, Department of Pediatric Sciences and Public Health, University of Turin, Turin, ItalyDepartment of Pediatric Sciences and Public Health, University of Turin, Turin, ItalyPediatric Laboratory, Department of Pediatric Sciences and Public Health, University of Turin, Turin, ItalyDepartment of Pediatric Sciences and Public Health, University of Turin, Turin, ItalyNeonatology Unit, Department of Pediatric Sciences and Public Health, University of Turin, Turin, ItalyDepartment of Surgical Sciences, Obstetrics and Gynecology 1, University of Turin, Turin, ItalyDepartment of Pediatric Sciences and Public Health, University of Turin, Turin, ItalyBackground: Placenta shows high transcription levels of human endogenous retroviruses (HERVs) that are overexpressed during embryonic and fetal development.Methods: In order to gather further information on the degree of HERV activation in maternal and fetal tissues we assessed the transcription levels of pol genes of HERV-H, -K, and -W in PBMCs of newborns and their mothers as well as in chorion (fetal part) and decidua basalis (maternal part) of the placenta using a real time PCR assay.Results: Transcripts of pol genes of the three HERV families were significantly higher in mononuclear cells from cord blood than from maternal blood and in the fetal part than in the maternal part of the placenta.Conclusions: The HERV over-expressions in cells and tissues of the offspring are further clues that they play pivotal physiologic roles during early life events and suggest that HERV-driven abnormalities of pregnancy and fetal development may derive mostly from the conceptus, not from the mother.https://www.frontiersin.org/article/10.3389/fped.2020.00244/fullHERV human endogenous retrovirusesPCRplacentacord bloodmRNA expression |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Massimiliano Bergallo Massimiliano Bergallo Luca Marozio Giovanni Botta Annalisa Tancredi Valentina Daprà Valentina Daprà Ilaria Galliano Ilaria Galliano Paola Montanari Paola Montanari Alessandra Coscia Chiara Benedetto Pier Angelo Tovo |
spellingShingle |
Massimiliano Bergallo Massimiliano Bergallo Luca Marozio Giovanni Botta Annalisa Tancredi Valentina Daprà Valentina Daprà Ilaria Galliano Ilaria Galliano Paola Montanari Paola Montanari Alessandra Coscia Chiara Benedetto Pier Angelo Tovo Human Endogenous Retroviruses Are Preferentially Expressed in Mononuclear Cells From Cord Blood Than From Maternal Blood and in the Fetal Part of Placenta Frontiers in Pediatrics HERV human endogenous retroviruses PCR placenta cord blood mRNA expression |
author_facet |
Massimiliano Bergallo Massimiliano Bergallo Luca Marozio Giovanni Botta Annalisa Tancredi Valentina Daprà Valentina Daprà Ilaria Galliano Ilaria Galliano Paola Montanari Paola Montanari Alessandra Coscia Chiara Benedetto Pier Angelo Tovo |
author_sort |
Massimiliano Bergallo |
title |
Human Endogenous Retroviruses Are Preferentially Expressed in Mononuclear Cells From Cord Blood Than From Maternal Blood and in the Fetal Part of Placenta |
title_short |
Human Endogenous Retroviruses Are Preferentially Expressed in Mononuclear Cells From Cord Blood Than From Maternal Blood and in the Fetal Part of Placenta |
title_full |
Human Endogenous Retroviruses Are Preferentially Expressed in Mononuclear Cells From Cord Blood Than From Maternal Blood and in the Fetal Part of Placenta |
title_fullStr |
Human Endogenous Retroviruses Are Preferentially Expressed in Mononuclear Cells From Cord Blood Than From Maternal Blood and in the Fetal Part of Placenta |
title_full_unstemmed |
Human Endogenous Retroviruses Are Preferentially Expressed in Mononuclear Cells From Cord Blood Than From Maternal Blood and in the Fetal Part of Placenta |
title_sort |
human endogenous retroviruses are preferentially expressed in mononuclear cells from cord blood than from maternal blood and in the fetal part of placenta |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Pediatrics |
issn |
2296-2360 |
publishDate |
2020-05-01 |
description |
Background: Placenta shows high transcription levels of human endogenous retroviruses (HERVs) that are overexpressed during embryonic and fetal development.Methods: In order to gather further information on the degree of HERV activation in maternal and fetal tissues we assessed the transcription levels of pol genes of HERV-H, -K, and -W in PBMCs of newborns and their mothers as well as in chorion (fetal part) and decidua basalis (maternal part) of the placenta using a real time PCR assay.Results: Transcripts of pol genes of the three HERV families were significantly higher in mononuclear cells from cord blood than from maternal blood and in the fetal part than in the maternal part of the placenta.Conclusions: The HERV over-expressions in cells and tissues of the offspring are further clues that they play pivotal physiologic roles during early life events and suggest that HERV-driven abnormalities of pregnancy and fetal development may derive mostly from the conceptus, not from the mother. |
topic |
HERV human endogenous retroviruses PCR placenta cord blood mRNA expression |
url |
https://www.frontiersin.org/article/10.3389/fped.2020.00244/full |
work_keys_str_mv |
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