Age Effects on Vascular Smooth Muscle: An Engineered Tissue Approach
Tissue engineering of blood vessels offers a potential new therapy for patients with vascular occlusive disease. In addition, tissue engineering technologies offer the opportunity to study the biology of vascular cells in a biomimetic, three-dimensional environment. A model for vascular tissue engin...
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2005-08-01
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Series: | Cell Transplantation |
Online Access: | https://doi.org/10.3727/000000005783982918 |
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doaj-5fd0ddfa28244d6aaea6dc6a4a01ad8e2020-11-25T03:38:22ZengSAGE PublishingCell Transplantation0963-68971555-38922005-08-011410.3727/000000005783982918Age Effects on Vascular Smooth Muscle: An Engineered Tissue ApproachAmy Solan0Laura Niklason M.D., Ph.D.1Department of Biomedical Engineering, Duke University, Durham, NC, USADepartment of Anesthesiology, Duke University Medical Center, Durham, NC, USATissue engineering of blood vessels offers a potential new therapy for patients with vascular occlusive disease. In addition, tissue engineering technologies offer the opportunity to study the biology of vascular cells in a biomimetic, three-dimensional environment. A model for vascular tissue engineering was used to study the effects of vascular cell age on extracellular matrix (ECM) deposition, cellular mitosis, and protein synthesis under controlled conditions in vitro. Blood vessels were grown using a three-dimensional polyglycolic acid (PGA) mesh that was seeded with either infant or adult porcine vascular smooth muscle cells. Mechanical forces in the form of pulsatile radial distension were applied for the duration of the 7-week growth period. Overall, infant cells exhibited higher levels of cellular proliferation, ECM deposition, and remodeling activity than cells derived from adult animals. In addition, vessels cultured from infant cells had enhanced physical properties compared to vessels cultured from adult cells. The differentiation state of the smooth muscle cells in the infant and adult constructs was unchanged from the native state. However, the levels of immature pro-collagen, although undetectable in the vessels grown from adult cells, were similar in native vessels and in vessels grown with infant cells. These studies have important implications for the study of aging and vascular disease and remodeling, as well as for the field of tissue engineering.https://doi.org/10.3727/000000005783982918 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Amy Solan Laura Niklason M.D., Ph.D. |
spellingShingle |
Amy Solan Laura Niklason M.D., Ph.D. Age Effects on Vascular Smooth Muscle: An Engineered Tissue Approach Cell Transplantation |
author_facet |
Amy Solan Laura Niklason M.D., Ph.D. |
author_sort |
Amy Solan |
title |
Age Effects on Vascular Smooth Muscle: An Engineered Tissue Approach |
title_short |
Age Effects on Vascular Smooth Muscle: An Engineered Tissue Approach |
title_full |
Age Effects on Vascular Smooth Muscle: An Engineered Tissue Approach |
title_fullStr |
Age Effects on Vascular Smooth Muscle: An Engineered Tissue Approach |
title_full_unstemmed |
Age Effects on Vascular Smooth Muscle: An Engineered Tissue Approach |
title_sort |
age effects on vascular smooth muscle: an engineered tissue approach |
publisher |
SAGE Publishing |
series |
Cell Transplantation |
issn |
0963-6897 1555-3892 |
publishDate |
2005-08-01 |
description |
Tissue engineering of blood vessels offers a potential new therapy for patients with vascular occlusive disease. In addition, tissue engineering technologies offer the opportunity to study the biology of vascular cells in a biomimetic, three-dimensional environment. A model for vascular tissue engineering was used to study the effects of vascular cell age on extracellular matrix (ECM) deposition, cellular mitosis, and protein synthesis under controlled conditions in vitro. Blood vessels were grown using a three-dimensional polyglycolic acid (PGA) mesh that was seeded with either infant or adult porcine vascular smooth muscle cells. Mechanical forces in the form of pulsatile radial distension were applied for the duration of the 7-week growth period. Overall, infant cells exhibited higher levels of cellular proliferation, ECM deposition, and remodeling activity than cells derived from adult animals. In addition, vessels cultured from infant cells had enhanced physical properties compared to vessels cultured from adult cells. The differentiation state of the smooth muscle cells in the infant and adult constructs was unchanged from the native state. However, the levels of immature pro-collagen, although undetectable in the vessels grown from adult cells, were similar in native vessels and in vessels grown with infant cells. These studies have important implications for the study of aging and vascular disease and remodeling, as well as for the field of tissue engineering. |
url |
https://doi.org/10.3727/000000005783982918 |
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