Long Non-Coding RNA USP2-AS1 Accelerates Cell Proliferation and Migration in Ovarian Cancer by Sponging miR-520d-3p and Up-Regulating KIAA1522

• Main Authors: Guo B, Yu L, Sun Y, Yao N, Ma L
• Format: Article
• Language: English
• Published: Dove Medical Press 2020-10-01
• Series: Cancer Management and Research
• Subjects: usp2-as1; mir-520d-3p; kiaa1522 and ovarian cancer
• Online Access: https://www.dovepress.com/long-non-coding-rna-usp2-as1-accelerates-cell-proliferation-and-migrat-peer-reviewed-article-CMAR
• ISSN: 1179-1322

Bingqin Guo,1 Lan Yu,1 Yanhong Sun,2 Nan Yao,1 Li Ma1 1Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233030, Anhui, People’s Republic of China; 2Department of Obstetrics and Gynecology, Huangshan People’s Hospital of Anhui Province, Huangshan, Anhui 245000, People’s Republic of ChinaCorrespondence: Lan YuDepartment of Pathology, The First Affiliated Hospital of Bengbu Medical College, 287 Changhuai Road, Bengbu 233030, Anhui, People’s Republic of ChinaTel [phone]Fax [phone]Email [e-mail]: Ovarian cancer is one of the malignant tumors attacking the female reproductive system. Currently, increasing studies have clearly determined the importance of long non-coding RNAs (lncRNAs) in various human cancers including ovarian cancer. However, the role and in-depth mechanism of ubiquitin specific peptidase 2 antisense RNA 1 (USP2-AS1) in ovarian cancer have been not reported yet.Purpose: We were absorbed into exploring the character of USP2-AS1 in ovarian cancer.Methods: RT-qPCR analysis reflected gene expression. The GEPIA database provided further evidences, and bioinformatics tools analyzed the potential molecules downstream USP2-AS1 in ovarian cancer. The changes on ovarian cancer cellular functions were assessed via EdU, TUNEL, JC-1 and transwell assays. RNA pull down, RIP and luciferase reporter assays estimated molecule interactions.Results: USP2-AS1 was obviously up-regulated in ovarian cancer tissues and cell lines. Inhibiting USP2-AS1 had anti-proliferation, pro-apoptosis, and anti-migration effects on ovarian cancer cells. Furthermore, we confirmed that USP2-AS1 sequestered miR-520d-3p to enhance KIAA1522. In addition, miR-520d-3p silence reversed the effect of depleted USP2-AS1 on ovarian cancer cellular behaviors, while such reversion was then abolished by KIAA1522 knockdown.Conclusion: USP2-AS1 facilitated ovarian cancer progression via miR-520d-3p/KIAA1522 axis, implying USP2-AS1 as a new perspective for the treatment of ovarian cancer.Keywords: USP2-AS1, miR-520d-3p, KIAA1522, ovarian cancer