Adipose tissue endocannabinoid system gene expression: depot differences and effects of diet and exercise
<p>Abstract</p> <p>Background</p> <p>Alterations of endocannabinoid system in adipose tissue play an important role in lipid regulation and metabolic dysfunction associated with obesity. The purpose of this study was to determine whether gene expression levels of cannab...
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doaj-5fd89a18a9c8414789b7028791cc65172020-11-25T00:20:32ZengBMCLipids in Health and Disease1476-511X2011-10-0110119410.1186/1476-511X-10-194Adipose tissue endocannabinoid system gene expression: depot differences and effects of diet and exerciseYang RongzeWang XuewenDisanzo Beth LYou TongjianGong Dawei<p>Abstract</p> <p>Background</p> <p>Alterations of endocannabinoid system in adipose tissue play an important role in lipid regulation and metabolic dysfunction associated with obesity. The purpose of this study was to determine whether gene expression levels of cannabinoid type 1 receptor (CB1) and fatty acid amide hydrolase (FAAH) are different in subcutaneous abdominal and gluteal adipose tissue, and whether hypocaloric diet and aerobic exercise influence subcutaneous adipose tissue CB1 and FAAH gene expression in obese women.</p> <p>Methods</p> <p>Thirty overweight or obese, middle-aged women (BMI = 34.3 ± 0.8 kg/m<sup>2</sup>, age = 59 ± 1 years) underwent one of three 20-week weight loss interventions: caloric restriction only (CR, N = 9), caloric restriction plus moderate-intensity aerobic exercise (CRM, 45-50% HRR, N = 13), or caloric restriction plus vigorous-intensity aerobic exercise (CRV, 70-75% HRR, N = 8). Subcutaneous abdominal and gluteal adipose tissue samples were collected before and after the interventions to measure CB1 and FAAH gene expression.</p> <p>Results</p> <p>At baseline, FAAH gene expression was higher in abdominal, compared to gluteal adipose tissue (2.08 ± 0.11 vs. 1.78 ± 0.10, expressed as target gene/β-actin mRNA ratio × 10<sup>-3</sup>, P < 0.05). Compared to pre-intervention, CR did not change abdominal, but decreased gluteal CB1 (Δ = -0.82 ± 0.25, P < 0.05) and FAAH (Δ = -0.49 ± 0.14, P < 0.05) gene expression. CRM or CRV alone did not change adipose tissue CB1 and FAAH gene expression. However, combined CRM and CRV (CRM+CRV) decreased abdominal adipose tissue FAAH gene expression (Δ = -0.37 ± 0.18, P < 0.05). The changes in gluteal CB1 and abdominal FAAH gene expression levels in the CR alone and the CRM+CRV group were different (P < 0.05) or tended to be different (P = 0.10).</p> <p>Conclusions</p> <p>There are depot differences in subcutaneous adipose tissue endocannabinoid system gene expression in obese individuals. Aerobic exercise training may preferentially modulate abdominal adipose tissue endocannabinoid-related gene expression during dietary weight loss.</p> <p>Trial Registration</p> <p>ClinicalTrials.gov: <a href="http://www.clinicaltrials.gov/ct2/show/NCT00664729">NCT00664729</a>.</p> http://www.lipidworld.com/content/10/1/194Cannabinoid Type 1 ReceptorFatty Acid Amide HydrolaseFat DepotsDietExercise |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yang Rongze Wang Xuewen Disanzo Beth L You Tongjian Gong Dawei |
spellingShingle |
Yang Rongze Wang Xuewen Disanzo Beth L You Tongjian Gong Dawei Adipose tissue endocannabinoid system gene expression: depot differences and effects of diet and exercise Lipids in Health and Disease Cannabinoid Type 1 Receptor Fatty Acid Amide Hydrolase Fat Depots Diet Exercise |
author_facet |
Yang Rongze Wang Xuewen Disanzo Beth L You Tongjian Gong Dawei |
author_sort |
Yang Rongze |
title |
Adipose tissue endocannabinoid system gene expression: depot differences and effects of diet and exercise |
title_short |
Adipose tissue endocannabinoid system gene expression: depot differences and effects of diet and exercise |
title_full |
Adipose tissue endocannabinoid system gene expression: depot differences and effects of diet and exercise |
title_fullStr |
Adipose tissue endocannabinoid system gene expression: depot differences and effects of diet and exercise |
title_full_unstemmed |
Adipose tissue endocannabinoid system gene expression: depot differences and effects of diet and exercise |
title_sort |
adipose tissue endocannabinoid system gene expression: depot differences and effects of diet and exercise |
publisher |
BMC |
series |
Lipids in Health and Disease |
issn |
1476-511X |
publishDate |
2011-10-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Alterations of endocannabinoid system in adipose tissue play an important role in lipid regulation and metabolic dysfunction associated with obesity. The purpose of this study was to determine whether gene expression levels of cannabinoid type 1 receptor (CB1) and fatty acid amide hydrolase (FAAH) are different in subcutaneous abdominal and gluteal adipose tissue, and whether hypocaloric diet and aerobic exercise influence subcutaneous adipose tissue CB1 and FAAH gene expression in obese women.</p> <p>Methods</p> <p>Thirty overweight or obese, middle-aged women (BMI = 34.3 ± 0.8 kg/m<sup>2</sup>, age = 59 ± 1 years) underwent one of three 20-week weight loss interventions: caloric restriction only (CR, N = 9), caloric restriction plus moderate-intensity aerobic exercise (CRM, 45-50% HRR, N = 13), or caloric restriction plus vigorous-intensity aerobic exercise (CRV, 70-75% HRR, N = 8). Subcutaneous abdominal and gluteal adipose tissue samples were collected before and after the interventions to measure CB1 and FAAH gene expression.</p> <p>Results</p> <p>At baseline, FAAH gene expression was higher in abdominal, compared to gluteal adipose tissue (2.08 ± 0.11 vs. 1.78 ± 0.10, expressed as target gene/β-actin mRNA ratio × 10<sup>-3</sup>, P < 0.05). Compared to pre-intervention, CR did not change abdominal, but decreased gluteal CB1 (Δ = -0.82 ± 0.25, P < 0.05) and FAAH (Δ = -0.49 ± 0.14, P < 0.05) gene expression. CRM or CRV alone did not change adipose tissue CB1 and FAAH gene expression. However, combined CRM and CRV (CRM+CRV) decreased abdominal adipose tissue FAAH gene expression (Δ = -0.37 ± 0.18, P < 0.05). The changes in gluteal CB1 and abdominal FAAH gene expression levels in the CR alone and the CRM+CRV group were different (P < 0.05) or tended to be different (P = 0.10).</p> <p>Conclusions</p> <p>There are depot differences in subcutaneous adipose tissue endocannabinoid system gene expression in obese individuals. Aerobic exercise training may preferentially modulate abdominal adipose tissue endocannabinoid-related gene expression during dietary weight loss.</p> <p>Trial Registration</p> <p>ClinicalTrials.gov: <a href="http://www.clinicaltrials.gov/ct2/show/NCT00664729">NCT00664729</a>.</p> |
topic |
Cannabinoid Type 1 Receptor Fatty Acid Amide Hydrolase Fat Depots Diet Exercise |
url |
http://www.lipidworld.com/content/10/1/194 |
work_keys_str_mv |
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