Transcriptomic epidemiology of smoking: the effect of smoking on gene expression in lymphocytes
<p>Abstract</p> <p>Background</p> <p>This investigation offers insights into system-wide pathological processes induced in response to cigarette smoke exposure by determining its influences at the gene expression level.</p> <p>Methods</p> <p>We o...
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2010-07-01
|
Series: | BMC Medical Genomics |
Online Access: | http://www.biomedcentral.com/1755-8794/3/29 |
id |
doaj-5fe9103499c64bf884d0ab8fca3a1ed2 |
---|---|
record_format |
Article |
spelling |
doaj-5fe9103499c64bf884d0ab8fca3a1ed22021-04-02T10:52:59ZengBMCBMC Medical Genomics1755-87942010-07-01312910.1186/1755-8794-3-29Transcriptomic epidemiology of smoking: the effect of smoking on gene expression in lymphocytesAlmasy LauraMahaney Michael CKent Jack WDiego Vincent PDyer Thomas DGöring Harald HHJohnson Matthew PCurran Joanne ECharlesworth Jac CMacCluer Jean WMoses Eric KBlangero John<p>Abstract</p> <p>Background</p> <p>This investigation offers insights into system-wide pathological processes induced in response to cigarette smoke exposure by determining its influences at the gene expression level.</p> <p>Methods</p> <p>We obtained genome-wide quantitative transcriptional profiles from 1,240 individuals from the San Antonio Family Heart Study, including 297 current smokers. Using lymphocyte samples, we identified 20,413 transcripts with significantly detectable expression levels, including both known and predicted genes. Correlation between smoking and gene expression levels was determined using a regression model that allows for residual genetic effects.</p> <p>Results</p> <p>With a conservative false-discovery rate of 5% we identified 323 unique genes (342 transcripts) whose expression levels were significantly correlated with smoking behavior. These genes showed significant over-representation within a range of functional categories that correspond well with known smoking-related pathologies, including immune response, cell death, cancer, natural killer cell signaling and xenobiotic metabolism.</p> <p>Conclusions</p> <p>Our results indicate that not only individual genes but entire networks of gene interaction are influenced by cigarette smoking. This is the largest <it>in vivo </it>transcriptomic epidemiological study of smoking to date and reveals the significant and comprehensive influence of cigarette smoke, as an environmental variable, on the expression of genes. The central importance of this manuscript is to provide a summary of the relationships between gene expression and smoking in this exceptionally large cross-sectional data set.</p> http://www.biomedcentral.com/1755-8794/3/29 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Almasy Laura Mahaney Michael C Kent Jack W Diego Vincent P Dyer Thomas D Göring Harald HH Johnson Matthew P Curran Joanne E Charlesworth Jac C MacCluer Jean W Moses Eric K Blangero John |
spellingShingle |
Almasy Laura Mahaney Michael C Kent Jack W Diego Vincent P Dyer Thomas D Göring Harald HH Johnson Matthew P Curran Joanne E Charlesworth Jac C MacCluer Jean W Moses Eric K Blangero John Transcriptomic epidemiology of smoking: the effect of smoking on gene expression in lymphocytes BMC Medical Genomics |
author_facet |
Almasy Laura Mahaney Michael C Kent Jack W Diego Vincent P Dyer Thomas D Göring Harald HH Johnson Matthew P Curran Joanne E Charlesworth Jac C MacCluer Jean W Moses Eric K Blangero John |
author_sort |
Almasy Laura |
title |
Transcriptomic epidemiology of smoking: the effect of smoking on gene expression in lymphocytes |
title_short |
Transcriptomic epidemiology of smoking: the effect of smoking on gene expression in lymphocytes |
title_full |
Transcriptomic epidemiology of smoking: the effect of smoking on gene expression in lymphocytes |
title_fullStr |
Transcriptomic epidemiology of smoking: the effect of smoking on gene expression in lymphocytes |
title_full_unstemmed |
Transcriptomic epidemiology of smoking: the effect of smoking on gene expression in lymphocytes |
title_sort |
transcriptomic epidemiology of smoking: the effect of smoking on gene expression in lymphocytes |
publisher |
BMC |
series |
BMC Medical Genomics |
issn |
1755-8794 |
publishDate |
2010-07-01 |
description |
<p>Abstract</p> <p>Background</p> <p>This investigation offers insights into system-wide pathological processes induced in response to cigarette smoke exposure by determining its influences at the gene expression level.</p> <p>Methods</p> <p>We obtained genome-wide quantitative transcriptional profiles from 1,240 individuals from the San Antonio Family Heart Study, including 297 current smokers. Using lymphocyte samples, we identified 20,413 transcripts with significantly detectable expression levels, including both known and predicted genes. Correlation between smoking and gene expression levels was determined using a regression model that allows for residual genetic effects.</p> <p>Results</p> <p>With a conservative false-discovery rate of 5% we identified 323 unique genes (342 transcripts) whose expression levels were significantly correlated with smoking behavior. These genes showed significant over-representation within a range of functional categories that correspond well with known smoking-related pathologies, including immune response, cell death, cancer, natural killer cell signaling and xenobiotic metabolism.</p> <p>Conclusions</p> <p>Our results indicate that not only individual genes but entire networks of gene interaction are influenced by cigarette smoking. This is the largest <it>in vivo </it>transcriptomic epidemiological study of smoking to date and reveals the significant and comprehensive influence of cigarette smoke, as an environmental variable, on the expression of genes. The central importance of this manuscript is to provide a summary of the relationships between gene expression and smoking in this exceptionally large cross-sectional data set.</p> |
url |
http://www.biomedcentral.com/1755-8794/3/29 |
work_keys_str_mv |
AT almasylaura transcriptomicepidemiologyofsmokingtheeffectofsmokingongeneexpressioninlymphocytes AT mahaneymichaelc transcriptomicepidemiologyofsmokingtheeffectofsmokingongeneexpressioninlymphocytes AT kentjackw transcriptomicepidemiologyofsmokingtheeffectofsmokingongeneexpressioninlymphocytes AT diegovincentp transcriptomicepidemiologyofsmokingtheeffectofsmokingongeneexpressioninlymphocytes AT dyerthomasd transcriptomicepidemiologyofsmokingtheeffectofsmokingongeneexpressioninlymphocytes AT goringharaldhh transcriptomicepidemiologyofsmokingtheeffectofsmokingongeneexpressioninlymphocytes AT johnsonmatthewp transcriptomicepidemiologyofsmokingtheeffectofsmokingongeneexpressioninlymphocytes AT curranjoannee transcriptomicepidemiologyofsmokingtheeffectofsmokingongeneexpressioninlymphocytes AT charlesworthjacc transcriptomicepidemiologyofsmokingtheeffectofsmokingongeneexpressioninlymphocytes AT maccluerjeanw transcriptomicepidemiologyofsmokingtheeffectofsmokingongeneexpressioninlymphocytes AT moseserick transcriptomicepidemiologyofsmokingtheeffectofsmokingongeneexpressioninlymphocytes AT blangerojohn transcriptomicepidemiologyofsmokingtheeffectofsmokingongeneexpressioninlymphocytes |
_version_ |
1724166427338866688 |