Development and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the detection of mink circovirus infection
Abstract Background Mink circovirus (MiCV) is a newly discovered pathogen associated with mink diarrhea. The prevalence and economic importance of this virus remain poorly understood, and no specific serological assay has been developed for the diagnosis of MiCV infection. Results In this study, a r...
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doaj-5ff360bc3226465fa0e9ce73eb327d002020-11-24T21:52:50ZengBMCBMC Veterinary Research1746-61482018-01-011411810.1186/s12917-018-1337-zDevelopment and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the detection of mink circovirus infectionJunwei Ge0Xingyang Cui1Yunjia Shi2Lili Zhao3Chengwei Wei4Shanshan Wen5Shuang Xia6Hongyan Chen7College of Veterinary Medicine, Northeast Agricultural UniversityCollege of Veterinary Medicine, Northeast Agricultural UniversityCollege of Veterinary Medicine, Northeast Agricultural UniversityLaboratory Animal and Comparative Medicine Unit, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural SciencesCollege of Veterinary Medicine, Northeast Agricultural UniversityCollege of Veterinary Medicine, Northeast Agricultural UniversityCollege of Veterinary Medicine, Northeast Agricultural UniversityLaboratory Animal and Comparative Medicine Unit, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural SciencesAbstract Background Mink circovirus (MiCV) is a newly discovered pathogen associated with mink diarrhea. The prevalence and economic importance of this virus remain poorly understood, and no specific serological assay has been developed for the diagnosis of MiCV infection. Results In this study, a recombinant capsid protein antigen expressed in Escherichia coli was utilized to establish an indirect enzyme-linked immunosorbent assay (iELISA). Results revealed that the assay had no cross-reactivity with other related pathogens, and the respective sensitivity and specificity of the proposed iELISA were 92.31% and 91.67% compared with those obtained of Western blot on 138 serum samples from minks. The correlation coefficient between iELISA and Western blot was 0.838 (p > 0.05). iELISA was applied to detect MiCV antibodies in 683 clinical serum samples from different farms from the major mink industry province in China, and 21 of 24 farms with 163 of 683 (23.87%) individuals were tested positive for MiCV antibodies. The positive rates of each of the 21 flocks ranged from 2.33% to 73.68%. Conclusions These results indicated that iELISA was a sensitive and specific method suitable for the large-scale detection of MiCV infections in mink. This study provided an effective method for the serological diagnosis and positive rate investigation of MiCV infection.http://link.springer.com/article/10.1186/s12917-018-1337-zMink circovirusCapsid proteinProkaryotic expressionIndirect ELISASerological survey |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Junwei Ge Xingyang Cui Yunjia Shi Lili Zhao Chengwei Wei Shanshan Wen Shuang Xia Hongyan Chen |
spellingShingle |
Junwei Ge Xingyang Cui Yunjia Shi Lili Zhao Chengwei Wei Shanshan Wen Shuang Xia Hongyan Chen Development and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the detection of mink circovirus infection BMC Veterinary Research Mink circovirus Capsid protein Prokaryotic expression Indirect ELISA Serological survey |
author_facet |
Junwei Ge Xingyang Cui Yunjia Shi Lili Zhao Chengwei Wei Shanshan Wen Shuang Xia Hongyan Chen |
author_sort |
Junwei Ge |
title |
Development and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the detection of mink circovirus infection |
title_short |
Development and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the detection of mink circovirus infection |
title_full |
Development and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the detection of mink circovirus infection |
title_fullStr |
Development and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the detection of mink circovirus infection |
title_full_unstemmed |
Development and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the detection of mink circovirus infection |
title_sort |
development and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the detection of mink circovirus infection |
publisher |
BMC |
series |
BMC Veterinary Research |
issn |
1746-6148 |
publishDate |
2018-01-01 |
description |
Abstract Background Mink circovirus (MiCV) is a newly discovered pathogen associated with mink diarrhea. The prevalence and economic importance of this virus remain poorly understood, and no specific serological assay has been developed for the diagnosis of MiCV infection. Results In this study, a recombinant capsid protein antigen expressed in Escherichia coli was utilized to establish an indirect enzyme-linked immunosorbent assay (iELISA). Results revealed that the assay had no cross-reactivity with other related pathogens, and the respective sensitivity and specificity of the proposed iELISA were 92.31% and 91.67% compared with those obtained of Western blot on 138 serum samples from minks. The correlation coefficient between iELISA and Western blot was 0.838 (p > 0.05). iELISA was applied to detect MiCV antibodies in 683 clinical serum samples from different farms from the major mink industry province in China, and 21 of 24 farms with 163 of 683 (23.87%) individuals were tested positive for MiCV antibodies. The positive rates of each of the 21 flocks ranged from 2.33% to 73.68%. Conclusions These results indicated that iELISA was a sensitive and specific method suitable for the large-scale detection of MiCV infections in mink. This study provided an effective method for the serological diagnosis and positive rate investigation of MiCV infection. |
topic |
Mink circovirus Capsid protein Prokaryotic expression Indirect ELISA Serological survey |
url |
http://link.springer.com/article/10.1186/s12917-018-1337-z |
work_keys_str_mv |
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