Qingchang Wenzhong Decoction Accelerates Intestinal Mucosal Healing Through Modulation of Dysregulated Gut Microbiome, Intestinal Barrier and Immune Responses in Mice
Inflammatory bowel disease (IBD), a group of multifactorial and inflammatory infirmities, is closely associated with dysregulation of gut microbiota and host metabolome, but effective treatments are currently limited. Qingchang Wenzhong Decoction (QCWZD) is an effective and classical traditional her...
Main Authors: | , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2021-09-01
|
Series: | Frontiers in Pharmacology |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2021.738152/full |
id |
doaj-5ffc95e2986a48d3a760004286eea15f |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhongmei Sun Zhongmei Sun Junxiang Li Wenting Wang Yuyue Liu Jia Liu Hui Jiang Qiongqiong Lu Panghua Ding Rui Shi Xingjie Zhao Wenjing Yuan Xiang Tan Xiaojun Shi Yunqi Xing Tangyou Mao |
spellingShingle |
Zhongmei Sun Zhongmei Sun Junxiang Li Wenting Wang Yuyue Liu Jia Liu Hui Jiang Qiongqiong Lu Panghua Ding Rui Shi Xingjie Zhao Wenjing Yuan Xiang Tan Xiaojun Shi Yunqi Xing Tangyou Mao Qingchang Wenzhong Decoction Accelerates Intestinal Mucosal Healing Through Modulation of Dysregulated Gut Microbiome, Intestinal Barrier and Immune Responses in Mice Frontiers in Pharmacology inflammatory bowel disease Qingchang Wenzhong decoction mucosal healing gut microbiota tryptophan metabolism intestinal stem cells |
author_facet |
Zhongmei Sun Zhongmei Sun Junxiang Li Wenting Wang Yuyue Liu Jia Liu Hui Jiang Qiongqiong Lu Panghua Ding Rui Shi Xingjie Zhao Wenjing Yuan Xiang Tan Xiaojun Shi Yunqi Xing Tangyou Mao |
author_sort |
Zhongmei Sun |
title |
Qingchang Wenzhong Decoction Accelerates Intestinal Mucosal Healing Through Modulation of Dysregulated Gut Microbiome, Intestinal Barrier and Immune Responses in Mice |
title_short |
Qingchang Wenzhong Decoction Accelerates Intestinal Mucosal Healing Through Modulation of Dysregulated Gut Microbiome, Intestinal Barrier and Immune Responses in Mice |
title_full |
Qingchang Wenzhong Decoction Accelerates Intestinal Mucosal Healing Through Modulation of Dysregulated Gut Microbiome, Intestinal Barrier and Immune Responses in Mice |
title_fullStr |
Qingchang Wenzhong Decoction Accelerates Intestinal Mucosal Healing Through Modulation of Dysregulated Gut Microbiome, Intestinal Barrier and Immune Responses in Mice |
title_full_unstemmed |
Qingchang Wenzhong Decoction Accelerates Intestinal Mucosal Healing Through Modulation of Dysregulated Gut Microbiome, Intestinal Barrier and Immune Responses in Mice |
title_sort |
qingchang wenzhong decoction accelerates intestinal mucosal healing through modulation of dysregulated gut microbiome, intestinal barrier and immune responses in mice |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Pharmacology |
issn |
1663-9812 |
publishDate |
2021-09-01 |
description |
Inflammatory bowel disease (IBD), a group of multifactorial and inflammatory infirmities, is closely associated with dysregulation of gut microbiota and host metabolome, but effective treatments are currently limited. Qingchang Wenzhong Decoction (QCWZD) is an effective and classical traditional herbal prescription for the treatment of IBD and has been proved to attenuate intestinal inflammation in a model of acute colitis. However, the role of QCWZD in recovery phase of colitis is unclear. Here, we demonstrated that mice treated with QCWZD showed a faster recovery from dextran sulfate sodium (DSS)-induced epithelial injury, accompanied by reduced mucosal inflammation and attenuated intestinal dysbiosis using bacterial 16S rRNA amplicon sequencing compared to those receiving sterile water. The protective effects of QCWZD are gut microbiota dependent, as demonstrated by fecal microbiome transplantation and antibiotics treatment. Gut microbes transferred from QCWZD-treated mice displayed a similar role in mucosal protection and epithelial regeneration as QCWZD on colitis in mice, and depletion of the gut microbiota through antibiotics treatments diminished the beneficial effects of QCWZD on colitis mice. Moreover, metabolomic analysis revealed metabolic profiles alternations in response to the gut microbiota reprogrammed by QCWZD intervention, especially enhanced tryptophan metabolism, which may further accelerate intestinal stem cells-mediated epithelial regeneration to protect the integrity of intestinal mucosa through activation of Wnt/β-catenin signals. Collectively, our results suggested that orally administrated QCWZD accelerates intestinal mucosal healing through the modulation of dysregulated gut microbiota and metabolism, thus regulating intestinal stem cells-mediated epithelial proliferation, and hold promise for novel microbial-based therapies in the treatment of IBD. |
topic |
inflammatory bowel disease Qingchang Wenzhong decoction mucosal healing gut microbiota tryptophan metabolism intestinal stem cells |
url |
https://www.frontiersin.org/articles/10.3389/fphar.2021.738152/full |
work_keys_str_mv |
AT zhongmeisun qingchangwenzhongdecoctionacceleratesintestinalmucosalhealingthroughmodulationofdysregulatedgutmicrobiomeintestinalbarrierandimmuneresponsesinmice AT zhongmeisun qingchangwenzhongdecoctionacceleratesintestinalmucosalhealingthroughmodulationofdysregulatedgutmicrobiomeintestinalbarrierandimmuneresponsesinmice AT junxiangli qingchangwenzhongdecoctionacceleratesintestinalmucosalhealingthroughmodulationofdysregulatedgutmicrobiomeintestinalbarrierandimmuneresponsesinmice AT wentingwang qingchangwenzhongdecoctionacceleratesintestinalmucosalhealingthroughmodulationofdysregulatedgutmicrobiomeintestinalbarrierandimmuneresponsesinmice AT yuyueliu qingchangwenzhongdecoctionacceleratesintestinalmucosalhealingthroughmodulationofdysregulatedgutmicrobiomeintestinalbarrierandimmuneresponsesinmice AT jialiu qingchangwenzhongdecoctionacceleratesintestinalmucosalhealingthroughmodulationofdysregulatedgutmicrobiomeintestinalbarrierandimmuneresponsesinmice AT huijiang qingchangwenzhongdecoctionacceleratesintestinalmucosalhealingthroughmodulationofdysregulatedgutmicrobiomeintestinalbarrierandimmuneresponsesinmice AT qiongqionglu qingchangwenzhongdecoctionacceleratesintestinalmucosalhealingthroughmodulationofdysregulatedgutmicrobiomeintestinalbarrierandimmuneresponsesinmice AT panghuading qingchangwenzhongdecoctionacceleratesintestinalmucosalhealingthroughmodulationofdysregulatedgutmicrobiomeintestinalbarrierandimmuneresponsesinmice AT ruishi qingchangwenzhongdecoctionacceleratesintestinalmucosalhealingthroughmodulationofdysregulatedgutmicrobiomeintestinalbarrierandimmuneresponsesinmice AT xingjiezhao qingchangwenzhongdecoctionacceleratesintestinalmucosalhealingthroughmodulationofdysregulatedgutmicrobiomeintestinalbarrierandimmuneresponsesinmice AT wenjingyuan qingchangwenzhongdecoctionacceleratesintestinalmucosalhealingthroughmodulationofdysregulatedgutmicrobiomeintestinalbarrierandimmuneresponsesinmice AT xiangtan qingchangwenzhongdecoctionacceleratesintestinalmucosalhealingthroughmodulationofdysregulatedgutmicrobiomeintestinalbarrierandimmuneresponsesinmice AT xiaojunshi qingchangwenzhongdecoctionacceleratesintestinalmucosalhealingthroughmodulationofdysregulatedgutmicrobiomeintestinalbarrierandimmuneresponsesinmice AT yunqixing qingchangwenzhongdecoctionacceleratesintestinalmucosalhealingthroughmodulationofdysregulatedgutmicrobiomeintestinalbarrierandimmuneresponsesinmice AT tangyoumao qingchangwenzhongdecoctionacceleratesintestinalmucosalhealingthroughmodulationofdysregulatedgutmicrobiomeintestinalbarrierandimmuneresponsesinmice |
_version_ |
1717764794153435136 |
spelling |
doaj-5ffc95e2986a48d3a760004286eea15f2021-09-07T04:47:45ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-09-011210.3389/fphar.2021.738152738152Qingchang Wenzhong Decoction Accelerates Intestinal Mucosal Healing Through Modulation of Dysregulated Gut Microbiome, Intestinal Barrier and Immune Responses in MiceZhongmei Sun0Zhongmei Sun1Junxiang Li2Wenting Wang3Yuyue Liu4Jia Liu5Hui Jiang6Qiongqiong Lu7Panghua Ding8Rui Shi9Xingjie Zhao10Wenjing Yuan11Xiang Tan12Xiaojun Shi13Yunqi Xing14Tangyou Mao15Department of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, ChinaGraduate School, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Traditional Chinese Medicine, Beijing Yangfangdian Hospital, Beijing, ChinaDepartment of Pathology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, ChinaDongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, ChinaInflammatory bowel disease (IBD), a group of multifactorial and inflammatory infirmities, is closely associated with dysregulation of gut microbiota and host metabolome, but effective treatments are currently limited. Qingchang Wenzhong Decoction (QCWZD) is an effective and classical traditional herbal prescription for the treatment of IBD and has been proved to attenuate intestinal inflammation in a model of acute colitis. However, the role of QCWZD in recovery phase of colitis is unclear. Here, we demonstrated that mice treated with QCWZD showed a faster recovery from dextran sulfate sodium (DSS)-induced epithelial injury, accompanied by reduced mucosal inflammation and attenuated intestinal dysbiosis using bacterial 16S rRNA amplicon sequencing compared to those receiving sterile water. The protective effects of QCWZD are gut microbiota dependent, as demonstrated by fecal microbiome transplantation and antibiotics treatment. Gut microbes transferred from QCWZD-treated mice displayed a similar role in mucosal protection and epithelial regeneration as QCWZD on colitis in mice, and depletion of the gut microbiota through antibiotics treatments diminished the beneficial effects of QCWZD on colitis mice. Moreover, metabolomic analysis revealed metabolic profiles alternations in response to the gut microbiota reprogrammed by QCWZD intervention, especially enhanced tryptophan metabolism, which may further accelerate intestinal stem cells-mediated epithelial regeneration to protect the integrity of intestinal mucosa through activation of Wnt/β-catenin signals. Collectively, our results suggested that orally administrated QCWZD accelerates intestinal mucosal healing through the modulation of dysregulated gut microbiota and metabolism, thus regulating intestinal stem cells-mediated epithelial proliferation, and hold promise for novel microbial-based therapies in the treatment of IBD.https://www.frontiersin.org/articles/10.3389/fphar.2021.738152/fullinflammatory bowel diseaseQingchang Wenzhong decoctionmucosal healinggut microbiotatryptophan metabolismintestinal stem cells |