Optimization of the Electrodeposition of Gold Nanoparticles for the Application of Highly Sensitive, Label-Free Biosensor
A highly sensitive electrochemical biosensor with a signal amplification platform of electrodeposited gold nanoparticle (AuNP) has been developed and characterized. The sizes of the synthesized AuNP were found to be critical for the performance of biosensor in which the sizes were dependent on HAuCl...
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doaj-5ffd0be7ba444448aa66d77d258113192020-11-24T21:52:47ZengMDPI AGBiosensors2079-63742019-03-01925010.3390/bios9020050bios9020050Optimization of the Electrodeposition of Gold Nanoparticles for the Application of Highly Sensitive, Label-Free BiosensorHao-Chun Chiang0Yanyan Wang1Qi Zhang2Kalle Levon3Department of Chemical and Biomolecular Engineering, NYU Tandon School of Engineering, Six Metrotech Center, Brooklyn, NY 11201, USAState Key Laboratory of Precision Measuring Technology & Instruments, School of Precision Instruments and Optoelectronics Engineering, Tianjin University, Tianjin 300072, ChinaDepartment of Chemical and Biomolecular Engineering, NYU Tandon School of Engineering, Six Metrotech Center, Brooklyn, NY 11201, USADepartment of Chemical and Biomolecular Engineering, NYU Tandon School of Engineering, Six Metrotech Center, Brooklyn, NY 11201, USAA highly sensitive electrochemical biosensor with a signal amplification platform of electrodeposited gold nanoparticle (AuNP) has been developed and characterized. The sizes of the synthesized AuNP were found to be critical for the performance of biosensor in which the sizes were dependent on HAuCl<sub>4</sub> and acid concentrations; as well as on scan cycles and scan rates in the gold electro-reduction step. Systematic investigations of the adsorption of proteins with different sizes from aqueous electrolyte solution onto the electrodeposited AuNP surface were performed with a potentiometric method and calibrated by design of experiment (DOE). The resulting amperometric glucose biosensors was demonstrated to have a low detection limit (>50 μM) and a wide linear range after optimization with AuNP electrodeposition.https://www.mdpi.com/2079-6374/9/2/50electrodepositionpotentiometrygold nanoparticlenanoparticle sizeprotein bindingenzyme activityoptimization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hao-Chun Chiang Yanyan Wang Qi Zhang Kalle Levon |
spellingShingle |
Hao-Chun Chiang Yanyan Wang Qi Zhang Kalle Levon Optimization of the Electrodeposition of Gold Nanoparticles for the Application of Highly Sensitive, Label-Free Biosensor Biosensors electrodeposition potentiometry gold nanoparticle nanoparticle size protein binding enzyme activity optimization |
author_facet |
Hao-Chun Chiang Yanyan Wang Qi Zhang Kalle Levon |
author_sort |
Hao-Chun Chiang |
title |
Optimization of the Electrodeposition of Gold Nanoparticles for the Application of Highly Sensitive, Label-Free Biosensor |
title_short |
Optimization of the Electrodeposition of Gold Nanoparticles for the Application of Highly Sensitive, Label-Free Biosensor |
title_full |
Optimization of the Electrodeposition of Gold Nanoparticles for the Application of Highly Sensitive, Label-Free Biosensor |
title_fullStr |
Optimization of the Electrodeposition of Gold Nanoparticles for the Application of Highly Sensitive, Label-Free Biosensor |
title_full_unstemmed |
Optimization of the Electrodeposition of Gold Nanoparticles for the Application of Highly Sensitive, Label-Free Biosensor |
title_sort |
optimization of the electrodeposition of gold nanoparticles for the application of highly sensitive, label-free biosensor |
publisher |
MDPI AG |
series |
Biosensors |
issn |
2079-6374 |
publishDate |
2019-03-01 |
description |
A highly sensitive electrochemical biosensor with a signal amplification platform of electrodeposited gold nanoparticle (AuNP) has been developed and characterized. The sizes of the synthesized AuNP were found to be critical for the performance of biosensor in which the sizes were dependent on HAuCl<sub>4</sub> and acid concentrations; as well as on scan cycles and scan rates in the gold electro-reduction step. Systematic investigations of the adsorption of proteins with different sizes from aqueous electrolyte solution onto the electrodeposited AuNP surface were performed with a potentiometric method and calibrated by design of experiment (DOE). The resulting amperometric glucose biosensors was demonstrated to have a low detection limit (>50 μM) and a wide linear range after optimization with AuNP electrodeposition. |
topic |
electrodeposition potentiometry gold nanoparticle nanoparticle size protein binding enzyme activity optimization |
url |
https://www.mdpi.com/2079-6374/9/2/50 |
work_keys_str_mv |
AT haochunchiang optimizationoftheelectrodepositionofgoldnanoparticlesfortheapplicationofhighlysensitivelabelfreebiosensor AT yanyanwang optimizationoftheelectrodepositionofgoldnanoparticlesfortheapplicationofhighlysensitivelabelfreebiosensor AT qizhang optimizationoftheelectrodepositionofgoldnanoparticlesfortheapplicationofhighlysensitivelabelfreebiosensor AT kallelevon optimizationoftheelectrodepositionofgoldnanoparticlesfortheapplicationofhighlysensitivelabelfreebiosensor |
_version_ |
1725874948513202176 |