RACK1 Acts as a Potential Tumor Promoter in Colorectal Cancer
Background. The receptor of activated protein kinase C 1 (RACK1) promotes the progression and invasion of several cancers. However, the role of RACK1 in the pathogenesis of colorectal cancer (CRC) has not been clearly defined. Herein, we aimed to investigate the biological role of RACK1 in CRC. Mate...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2019-01-01
|
Series: | Gastroenterology Research and Practice |
Online Access: | http://dx.doi.org/10.1155/2019/5625026 |
id |
doaj-60015adc953f4d7e800f0a277b189544 |
---|---|
record_format |
Article |
spelling |
doaj-60015adc953f4d7e800f0a277b1895442020-11-25T02:22:49ZengHindawi LimitedGastroenterology Research and Practice1687-61211687-630X2019-01-01201910.1155/2019/56250265625026RACK1 Acts as a Potential Tumor Promoter in Colorectal CancerXue-Yang Li0Yi Hu1Nian-Shuang Li2Jian-Hua Wan3Yin Zhu4Nong-Hua Lu5Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006 Jiangxi Province, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006 Jiangxi Province, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006 Jiangxi Province, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006 Jiangxi Province, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006 Jiangxi Province, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006 Jiangxi Province, ChinaBackground. The receptor of activated protein kinase C 1 (RACK1) promotes the progression and invasion of several cancers. However, the role of RACK1 in the pathogenesis of colorectal cancer (CRC) has not been clearly defined. Herein, we aimed to investigate the biological role of RACK1 in CRC. Materials and Methods. The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) dataset were searched, and the expression of RACK1 in CRC tissues and adjacent normal tissues was evaluated. Immunohistochemical staining was performed to detect the expression of RACK1 in human CRC, adenoma, and normal tissues. Western blotting was used to detect the expression of RACK1 in human CRC cell lines. Functional assays, such as BrdU, colony formation, and wound healing and transwell invasion assays, were used to explore the biological role of RACK1 in CRC. Results. RACK1 was upregulated in CRC tissues compared with its expression in adjacent normal tissues in TCGA and the GEO dataset (P<0.05). Moreover, RACK1 was significantly overexpressed in CRC and adenoma tissues compared with its expression in normal tissues (P<0.05). Loss-of-function experiments showed that RACK1 promoted cell proliferation, migration, and invasion in vitro. Conclusions. Our data indicated that RACK1, as an oncogene, markedly promoted the progression of CRC, which suggested that RACK1 is a potential therapeutic target for CRC management.http://dx.doi.org/10.1155/2019/5625026 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xue-Yang Li Yi Hu Nian-Shuang Li Jian-Hua Wan Yin Zhu Nong-Hua Lu |
spellingShingle |
Xue-Yang Li Yi Hu Nian-Shuang Li Jian-Hua Wan Yin Zhu Nong-Hua Lu RACK1 Acts as a Potential Tumor Promoter in Colorectal Cancer Gastroenterology Research and Practice |
author_facet |
Xue-Yang Li Yi Hu Nian-Shuang Li Jian-Hua Wan Yin Zhu Nong-Hua Lu |
author_sort |
Xue-Yang Li |
title |
RACK1 Acts as a Potential Tumor Promoter in Colorectal Cancer |
title_short |
RACK1 Acts as a Potential Tumor Promoter in Colorectal Cancer |
title_full |
RACK1 Acts as a Potential Tumor Promoter in Colorectal Cancer |
title_fullStr |
RACK1 Acts as a Potential Tumor Promoter in Colorectal Cancer |
title_full_unstemmed |
RACK1 Acts as a Potential Tumor Promoter in Colorectal Cancer |
title_sort |
rack1 acts as a potential tumor promoter in colorectal cancer |
publisher |
Hindawi Limited |
series |
Gastroenterology Research and Practice |
issn |
1687-6121 1687-630X |
publishDate |
2019-01-01 |
description |
Background. The receptor of activated protein kinase C 1 (RACK1) promotes the progression and invasion of several cancers. However, the role of RACK1 in the pathogenesis of colorectal cancer (CRC) has not been clearly defined. Herein, we aimed to investigate the biological role of RACK1 in CRC. Materials and Methods. The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) dataset were searched, and the expression of RACK1 in CRC tissues and adjacent normal tissues was evaluated. Immunohistochemical staining was performed to detect the expression of RACK1 in human CRC, adenoma, and normal tissues. Western blotting was used to detect the expression of RACK1 in human CRC cell lines. Functional assays, such as BrdU, colony formation, and wound healing and transwell invasion assays, were used to explore the biological role of RACK1 in CRC. Results. RACK1 was upregulated in CRC tissues compared with its expression in adjacent normal tissues in TCGA and the GEO dataset (P<0.05). Moreover, RACK1 was significantly overexpressed in CRC and adenoma tissues compared with its expression in normal tissues (P<0.05). Loss-of-function experiments showed that RACK1 promoted cell proliferation, migration, and invasion in vitro. Conclusions. Our data indicated that RACK1, as an oncogene, markedly promoted the progression of CRC, which suggested that RACK1 is a potential therapeutic target for CRC management. |
url |
http://dx.doi.org/10.1155/2019/5625026 |
work_keys_str_mv |
AT xueyangli rack1actsasapotentialtumorpromoterincolorectalcancer AT yihu rack1actsasapotentialtumorpromoterincolorectalcancer AT nianshuangli rack1actsasapotentialtumorpromoterincolorectalcancer AT jianhuawan rack1actsasapotentialtumorpromoterincolorectalcancer AT yinzhu rack1actsasapotentialtumorpromoterincolorectalcancer AT nonghualu rack1actsasapotentialtumorpromoterincolorectalcancer |
_version_ |
1724861558196011008 |