Enhancement of GAD Storage Stability with Immobilization on PDA-Coated Superparamagnetic Magnetite Nanoparticles
To improve the storage stability of glutamic acid decarboxylase (GAD), superparamagnetic magnetite (Fe<sub>3</sub>O<sub>4</sub>) nanoparticles were synthesized by co-precipitation method and coated with polydopamine (PDA) for GAD immobilization. Dynamic light scattering and t...
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doaj-601005085dad4b8690f44a42c664c4632020-11-25T00:34:30ZengMDPI AGCatalysts2073-43442019-11-0191196910.3390/catal9110969catal9110969Enhancement of GAD Storage Stability with Immobilization on PDA-Coated Superparamagnetic Magnetite NanoparticlesFarheen Zafar0Hong-peng Wang1Chang-jiang Lv2Muhammad-Haseeb Ullah3Chun-yan Liu4Yu-jiao Hua5Le-he Mei6Jun Huang7Zhejiang Provincial Key Lab for Chemical and Biological Processing Technology of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, ChinaZhejiang Provincial Key Lab for Chemical and Biological Processing Technology of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, ChinaZhejiang Provincial Key Lab for Chemical and Biological Processing Technology of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, ChinaZhejiang Provincial Key Lab for Chemical and Biological Processing Technology of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, ChinaZhejiang Provincial Key Lab for Chemical and Biological Processing Technology of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, ChinaZhejiang Provincial Key Lab for Chemical and Biological Processing Technology of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, ChinaSchool of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, ChinaZhejiang Provincial Key Lab for Chemical and Biological Processing Technology of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, ChinaTo improve the storage stability of glutamic acid decarboxylase (GAD), superparamagnetic magnetite (Fe<sub>3</sub>O<sub>4</sub>) nanoparticles were synthesized by co-precipitation method and coated with polydopamine (PDA) for GAD immobilization. Dynamic light scattering and transmission electron microscopy were used to determine size of the nanoparticles, which were approximately 10 nm, increasing to 15 nm after PDA-coating and to 20 nm upon GAD binding. Vibrational scanning measurements significantly represented the superparamagnetic behavior of the Fe<sub>3</sub>O<sub>4</sub>, and X-ray diffraction analysis confirmed that the crystalline structure before and after coating with PDA and the further immobilization of GAD remained the same. Thermogravimetric analysis and Fourier-transform infrared spectroscopy proved that the PDA-coating on Fe<sub>3</sub>O<sub>4</sub> and further immobilization of GAD were successful. After immobilization, the enzyme can be used with a relative specific activity of 40.7% after five successive uses. The immobilized enzyme retained relative specific activity of about 50.5% after 15 days of storage at 4 °C, while free enzyme showed no relative specific activity after two days of storage. The GAD immobilization on PDA-coated magnetite nanoparticles was reported for the improvement of enzyme storage stability for the first time.https://www.mdpi.com/2073-4344/9/11/969enzymeglutamic acid decarboxylase immobilizationmagnetitenanoparticlespolydopamine |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Farheen Zafar Hong-peng Wang Chang-jiang Lv Muhammad-Haseeb Ullah Chun-yan Liu Yu-jiao Hua Le-he Mei Jun Huang |
spellingShingle |
Farheen Zafar Hong-peng Wang Chang-jiang Lv Muhammad-Haseeb Ullah Chun-yan Liu Yu-jiao Hua Le-he Mei Jun Huang Enhancement of GAD Storage Stability with Immobilization on PDA-Coated Superparamagnetic Magnetite Nanoparticles Catalysts enzyme glutamic acid decarboxylase immobilization magnetite nanoparticles polydopamine |
author_facet |
Farheen Zafar Hong-peng Wang Chang-jiang Lv Muhammad-Haseeb Ullah Chun-yan Liu Yu-jiao Hua Le-he Mei Jun Huang |
author_sort |
Farheen Zafar |
title |
Enhancement of GAD Storage Stability with Immobilization on PDA-Coated Superparamagnetic Magnetite Nanoparticles |
title_short |
Enhancement of GAD Storage Stability with Immobilization on PDA-Coated Superparamagnetic Magnetite Nanoparticles |
title_full |
Enhancement of GAD Storage Stability with Immobilization on PDA-Coated Superparamagnetic Magnetite Nanoparticles |
title_fullStr |
Enhancement of GAD Storage Stability with Immobilization on PDA-Coated Superparamagnetic Magnetite Nanoparticles |
title_full_unstemmed |
Enhancement of GAD Storage Stability with Immobilization on PDA-Coated Superparamagnetic Magnetite Nanoparticles |
title_sort |
enhancement of gad storage stability with immobilization on pda-coated superparamagnetic magnetite nanoparticles |
publisher |
MDPI AG |
series |
Catalysts |
issn |
2073-4344 |
publishDate |
2019-11-01 |
description |
To improve the storage stability of glutamic acid decarboxylase (GAD), superparamagnetic magnetite (Fe<sub>3</sub>O<sub>4</sub>) nanoparticles were synthesized by co-precipitation method and coated with polydopamine (PDA) for GAD immobilization. Dynamic light scattering and transmission electron microscopy were used to determine size of the nanoparticles, which were approximately 10 nm, increasing to 15 nm after PDA-coating and to 20 nm upon GAD binding. Vibrational scanning measurements significantly represented the superparamagnetic behavior of the Fe<sub>3</sub>O<sub>4</sub>, and X-ray diffraction analysis confirmed that the crystalline structure before and after coating with PDA and the further immobilization of GAD remained the same. Thermogravimetric analysis and Fourier-transform infrared spectroscopy proved that the PDA-coating on Fe<sub>3</sub>O<sub>4</sub> and further immobilization of GAD were successful. After immobilization, the enzyme can be used with a relative specific activity of 40.7% after five successive uses. The immobilized enzyme retained relative specific activity of about 50.5% after 15 days of storage at 4 °C, while free enzyme showed no relative specific activity after two days of storage. The GAD immobilization on PDA-coated magnetite nanoparticles was reported for the improvement of enzyme storage stability for the first time. |
topic |
enzyme glutamic acid decarboxylase immobilization magnetite nanoparticles polydopamine |
url |
https://www.mdpi.com/2073-4344/9/11/969 |
work_keys_str_mv |
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