Huangqin flavonoid extraction for spinal cord injury in a rat model
Flavonoids from Huangqin (dried roots of Scutellaria baicalensis Georgi) have anti-inflammatory effects, and are considered useful for treatment of spinal cord injury. To verify this hypothesis, the T9–10 spinal cord segments of rats were damaged using Allen's method to establish a rat spinal c...
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Wolters Kluwer Medknow Publications
2018-01-01
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doaj-601336169fe44ba8a25ae1dbc47a4b6d2020-11-25T03:04:11ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742018-01-0113122200220810.4103/1673-5374.241472Huangqin flavonoid extraction for spinal cord injury in a rat modelQian ZhangLi-Xin ZhangJing AnLiang YanCui-Cui LiuJing-Jing ZhaoHao YangFlavonoids from Huangqin (dried roots of Scutellaria baicalensis Georgi) have anti-inflammatory effects, and are considered useful for treatment of spinal cord injury. To verify this hypothesis, the T9–10 spinal cord segments of rats were damaged using Allen's method to establish a rat spinal cord injury model. Before model establishment, Huangqin flavonoid extraction (12.5 g/kg) was administered intragastrically for 1 week until 28 days after model establishment. Methylprednisolone (30 mg/kg) was injected into the tail vein at 30 minutes after model establishment as a positive control. Basso, Beattie, and Bresnahan locomotor scale scores were used to assess hind limb motor function. Hematoxylin-eosin staining was used to detect pathological changes in the injured spinal cord. Immunofluorescence and western blot assays were performed to measure immunoreactivity and expression levels of brain-derived neurotrophic factor, neuronal marker neurofilament protein, microglial marker CD11b and astrocyte marker glial fibrillary acidic protein in the injured spinal cord. Huangqin flavonoid extraction markedly reduced spinal cord hematoma, inflammatory cell infiltration and cavities and scars, and increased the Basso, Beattie, and Bresnahan locomotor scale scores; these effects were identical to those of methylprednisolone. Huangqin flavonoid extraction also increased immunoreactivity and expression levels of brain-derived neurotrophic factor and neurofilament protein, and reduced immunoreactivity and expression levels of CD11b and glial fibrillary acidic protein, in the injured spinal cord. Overall, these data suggest that Huangqin flavonoid extraction can promote recovery of spinal cord injury by inducing brain-derived neurotrophic factor and neurofilament protein expression, reducing microglia activation and regulating reactive astrocytes.http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=12;spage=2200;epage=2208;aulast=Zhangnerve regeneration; spinal cord injury; flavonoids; Scutellaria baicalensis; neurofilament; brain-derived neurotrophic factor; astrocytes; microglia; neural regeneration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qian Zhang Li-Xin Zhang Jing An Liang Yan Cui-Cui Liu Jing-Jing Zhao Hao Yang |
spellingShingle |
Qian Zhang Li-Xin Zhang Jing An Liang Yan Cui-Cui Liu Jing-Jing Zhao Hao Yang Huangqin flavonoid extraction for spinal cord injury in a rat model Neural Regeneration Research nerve regeneration; spinal cord injury; flavonoids; Scutellaria baicalensis; neurofilament; brain-derived neurotrophic factor; astrocytes; microglia; neural regeneration |
author_facet |
Qian Zhang Li-Xin Zhang Jing An Liang Yan Cui-Cui Liu Jing-Jing Zhao Hao Yang |
author_sort |
Qian Zhang |
title |
Huangqin flavonoid extraction for spinal cord injury in a rat model |
title_short |
Huangqin flavonoid extraction for spinal cord injury in a rat model |
title_full |
Huangqin flavonoid extraction for spinal cord injury in a rat model |
title_fullStr |
Huangqin flavonoid extraction for spinal cord injury in a rat model |
title_full_unstemmed |
Huangqin flavonoid extraction for spinal cord injury in a rat model |
title_sort |
huangqin flavonoid extraction for spinal cord injury in a rat model |
publisher |
Wolters Kluwer Medknow Publications |
series |
Neural Regeneration Research |
issn |
1673-5374 |
publishDate |
2018-01-01 |
description |
Flavonoids from Huangqin (dried roots of Scutellaria baicalensis Georgi) have anti-inflammatory effects, and are considered useful for treatment of spinal cord injury. To verify this hypothesis, the T9–10 spinal cord segments of rats were damaged using Allen's method to establish a rat spinal cord injury model. Before model establishment, Huangqin flavonoid extraction (12.5 g/kg) was administered intragastrically for 1 week until 28 days after model establishment. Methylprednisolone (30 mg/kg) was injected into the tail vein at 30 minutes after model establishment as a positive control. Basso, Beattie, and Bresnahan locomotor scale scores were used to assess hind limb motor function. Hematoxylin-eosin staining was used to detect pathological changes in the injured spinal cord. Immunofluorescence and western blot assays were performed to measure immunoreactivity and expression levels of brain-derived neurotrophic factor, neuronal marker neurofilament protein, microglial marker CD11b and astrocyte marker glial fibrillary acidic protein in the injured spinal cord. Huangqin flavonoid extraction markedly reduced spinal cord hematoma, inflammatory cell infiltration and cavities and scars, and increased the Basso, Beattie, and Bresnahan locomotor scale scores; these effects were identical to those of methylprednisolone. Huangqin flavonoid extraction also increased immunoreactivity and expression levels of brain-derived neurotrophic factor and neurofilament protein, and reduced immunoreactivity and expression levels of CD11b and glial fibrillary acidic protein, in the injured spinal cord. Overall, these data suggest that Huangqin flavonoid extraction can promote recovery of spinal cord injury by inducing brain-derived neurotrophic factor and neurofilament protein expression, reducing microglia activation and regulating reactive astrocytes. |
topic |
nerve regeneration; spinal cord injury; flavonoids; Scutellaria baicalensis; neurofilament; brain-derived neurotrophic factor; astrocytes; microglia; neural regeneration |
url |
http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=12;spage=2200;epage=2208;aulast=Zhang |
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