Detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2

In this study, positive blood and organ samples were obtained from different mixed herds of sheep and cattle against ovine herpesvirus 2 (OvHV-2) infection. Target-positive DNA was sequenced and compared with worldwide distributed OvHV-2 sequences. Tegument gene (422 base pairs) and glycoprotein B (...

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Main Authors: Tuba Ç. Oğuzoğlu, Seçkin Salar, Ece Adıgüzel, Cansu Demirden, Onur Ülgenalp
Format: Article
Language:English
Published: AOSIS 2020-11-01
Series:Onderstepoort Journal of Veterinary Research
Subjects:
Online Access:https://ojvr.org/index.php/ojvr/article/view/1886
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spelling doaj-601a9547699b4f37a2a73e03c46296ea2020-11-25T04:08:40ZengAOSISOnderstepoort Journal of Veterinary Research0030-24652219-06352020-11-01871e1e410.4102/ojvr.v87i1.1886646Detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2Tuba Ç. Oğuzoğlu0Seçkin Salar1Ece Adıgüzel2Cansu Demirden3Onur Ülgenalp4Department of Virology, Faculty of Veterinary Medicine, Ankara University, AnkaraDepartment of Obstetric and Gnynecology, Faculty of Veterinary Medicine, Ankara University, AnkaraDepartment of Virology, Faculty of Veterinary Medicine, Ankara University, AnkaraDepartment of Virology, Faculty of Veterinary Medicine, Ankara University, AnkaraDepartment of Virology, Faculty of Veterinary Medicine, Ankara University, AnkaraIn this study, positive blood and organ samples were obtained from different mixed herds of sheep and cattle against ovine herpesvirus 2 (OvHV-2) infection. Target-positive DNA was sequenced and compared with worldwide distributed OvHV-2 sequences. Tegument gene (422 base pairs) and glycoprotein B (gB) gene (2800 base pairs) amplicons of OvHV-2 genome were used for understanding of epidemiology of malignant catarrhal fever (MCF) infection in Turkey. The results of nucleotide sequencing of polymerase chain reaction (PCR) products indicated presence of sheep-associated form for MCF infection in Turkey. Although the obtained sequences were genetically different from each other, it was found that genetic variations were limited.https://ojvr.org/index.php/ojvr/article/view/1886malignant catarrhal fevertegument genegb geneovhv-2turkey
collection DOAJ
language English
format Article
sources DOAJ
author Tuba Ç. Oğuzoğlu
Seçkin Salar
Ece Adıgüzel
Cansu Demirden
Onur Ülgenalp
spellingShingle Tuba Ç. Oğuzoğlu
Seçkin Salar
Ece Adıgüzel
Cansu Demirden
Onur Ülgenalp
Detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2
Onderstepoort Journal of Veterinary Research
malignant catarrhal fever
tegument gene
gb gene
ovhv-2
turkey
author_facet Tuba Ç. Oğuzoğlu
Seçkin Salar
Ece Adıgüzel
Cansu Demirden
Onur Ülgenalp
author_sort Tuba Ç. Oğuzoğlu
title Detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2
title_short Detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2
title_full Detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2
title_fullStr Detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2
title_full_unstemmed Detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2
title_sort detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gb gene sequences of ovhv-2
publisher AOSIS
series Onderstepoort Journal of Veterinary Research
issn 0030-2465
2219-0635
publishDate 2020-11-01
description In this study, positive blood and organ samples were obtained from different mixed herds of sheep and cattle against ovine herpesvirus 2 (OvHV-2) infection. Target-positive DNA was sequenced and compared with worldwide distributed OvHV-2 sequences. Tegument gene (422 base pairs) and glycoprotein B (gB) gene (2800 base pairs) amplicons of OvHV-2 genome were used for understanding of epidemiology of malignant catarrhal fever (MCF) infection in Turkey. The results of nucleotide sequencing of polymerase chain reaction (PCR) products indicated presence of sheep-associated form for MCF infection in Turkey. Although the obtained sequences were genetically different from each other, it was found that genetic variations were limited.
topic malignant catarrhal fever
tegument gene
gb gene
ovhv-2
turkey
url https://ojvr.org/index.php/ojvr/article/view/1886
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AT seckinsalar detectionandcharacterisationofsheepassociatedmalignantcatarrhalfeverinfectionfromruminantsbyusingtegumentandgbgenesequencesofovhv2
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