Detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2
In this study, positive blood and organ samples were obtained from different mixed herds of sheep and cattle against ovine herpesvirus 2 (OvHV-2) infection. Target-positive DNA was sequenced and compared with worldwide distributed OvHV-2 sequences. Tegument gene (422 base pairs) and glycoprotein B (...
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doaj-601a9547699b4f37a2a73e03c46296ea2020-11-25T04:08:40ZengAOSISOnderstepoort Journal of Veterinary Research0030-24652219-06352020-11-01871e1e410.4102/ojvr.v87i1.1886646Detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2Tuba Ç. Oğuzoğlu0Seçkin Salar1Ece Adıgüzel2Cansu Demirden3Onur Ülgenalp4Department of Virology, Faculty of Veterinary Medicine, Ankara University, AnkaraDepartment of Obstetric and Gnynecology, Faculty of Veterinary Medicine, Ankara University, AnkaraDepartment of Virology, Faculty of Veterinary Medicine, Ankara University, AnkaraDepartment of Virology, Faculty of Veterinary Medicine, Ankara University, AnkaraDepartment of Virology, Faculty of Veterinary Medicine, Ankara University, AnkaraIn this study, positive blood and organ samples were obtained from different mixed herds of sheep and cattle against ovine herpesvirus 2 (OvHV-2) infection. Target-positive DNA was sequenced and compared with worldwide distributed OvHV-2 sequences. Tegument gene (422 base pairs) and glycoprotein B (gB) gene (2800 base pairs) amplicons of OvHV-2 genome were used for understanding of epidemiology of malignant catarrhal fever (MCF) infection in Turkey. The results of nucleotide sequencing of polymerase chain reaction (PCR) products indicated presence of sheep-associated form for MCF infection in Turkey. Although the obtained sequences were genetically different from each other, it was found that genetic variations were limited.https://ojvr.org/index.php/ojvr/article/view/1886malignant catarrhal fevertegument genegb geneovhv-2turkey |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tuba Ç. Oğuzoğlu Seçkin Salar Ece Adıgüzel Cansu Demirden Onur Ülgenalp |
spellingShingle |
Tuba Ç. Oğuzoğlu Seçkin Salar Ece Adıgüzel Cansu Demirden Onur Ülgenalp Detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2 Onderstepoort Journal of Veterinary Research malignant catarrhal fever tegument gene gb gene ovhv-2 turkey |
author_facet |
Tuba Ç. Oğuzoğlu Seçkin Salar Ece Adıgüzel Cansu Demirden Onur Ülgenalp |
author_sort |
Tuba Ç. Oğuzoğlu |
title |
Detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2 |
title_short |
Detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2 |
title_full |
Detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2 |
title_fullStr |
Detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2 |
title_full_unstemmed |
Detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2 |
title_sort |
detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gb gene sequences of ovhv-2 |
publisher |
AOSIS |
series |
Onderstepoort Journal of Veterinary Research |
issn |
0030-2465 2219-0635 |
publishDate |
2020-11-01 |
description |
In this study, positive blood and organ samples were obtained from different mixed herds of sheep and cattle against ovine herpesvirus 2 (OvHV-2) infection. Target-positive DNA was sequenced and compared with worldwide distributed OvHV-2 sequences. Tegument gene (422 base pairs) and glycoprotein B (gB) gene (2800 base pairs) amplicons of OvHV-2 genome were used for understanding of epidemiology of malignant catarrhal fever (MCF) infection in Turkey. The results of nucleotide sequencing of polymerase chain reaction (PCR) products indicated presence of sheep-associated form for MCF infection in Turkey. Although the obtained sequences were genetically different from each other, it was found that genetic variations were limited. |
topic |
malignant catarrhal fever tegument gene gb gene ovhv-2 turkey |
url |
https://ojvr.org/index.php/ojvr/article/view/1886 |
work_keys_str_mv |
AT tubacoguzoglu detectionandcharacterisationofsheepassociatedmalignantcatarrhalfeverinfectionfromruminantsbyusingtegumentandgbgenesequencesofovhv2 AT seckinsalar detectionandcharacterisationofsheepassociatedmalignantcatarrhalfeverinfectionfromruminantsbyusingtegumentandgbgenesequencesofovhv2 AT eceadıguzel detectionandcharacterisationofsheepassociatedmalignantcatarrhalfeverinfectionfromruminantsbyusingtegumentandgbgenesequencesofovhv2 AT cansudemirden detectionandcharacterisationofsheepassociatedmalignantcatarrhalfeverinfectionfromruminantsbyusingtegumentandgbgenesequencesofovhv2 AT onurulgenalp detectionandcharacterisationofsheepassociatedmalignantcatarrhalfeverinfectionfromruminantsbyusingtegumentandgbgenesequencesofovhv2 |
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