Effects of in ovo injection of microalgae on hatchability, antioxidant and immunity-related genes expression, and post-hatch performance in broilers and Japanese quails
The effects of in ovo injection of Arthrospira (Spirulina) Platensis on hatchability, antioxidant and immunity-related genes expression, and hatchling performance of broilers and Japanese quails was assessed in 2 separate experiments. In experiment 1, a total of 240 fertilised quail eggs were divide...
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doaj-6034922663694b1bb2c784dbe2040b022021-07-26T14:50:59ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2021-01-0120198599410.1080/1828051X.2021.19105821910582Effects of in ovo injection of microalgae on hatchability, antioxidant and immunity-related genes expression, and post-hatch performance in broilers and Japanese quailsHosna Hajati0Mojtaba Zaghari1Omid Noori2Reza Negarandeh3Haniel Cedraz de Oliveira4Animal Science Research Department, East Azarbaijan Agricultural and Natural Resources Research and Education Center, AREEODepartment of Animal Science, College of Agriculture and Natural Resources, University of TehranDepartment of Animal Science, College of Agriculture and Natural Resources, University of TehranFaculty of Pharmacy, Mazandaran University of Medical SciencesDepartment of Animal Science, Universidade Federal de ViçosaThe effects of in ovo injection of Arthrospira (Spirulina) Platensis on hatchability, antioxidant and immunity-related genes expression, and hatchling performance of broilers and Japanese quails was assessed in 2 separate experiments. In experiment 1, a total of 240 fertilised quail eggs were divided into six groups: control (without injection), sham control (0.02 CC distilled water/egg), 0.75 mg Spirulina Platensis (SP)/egg, 1.5 mg SP/egg, 2.5 mg SP/egg, 3.5 mg SP/egg. In experiment 2, a total of 192 fertilised broiler breeder were divided into four groups: control, sham control (0.2 CC distilled water/egg), 25 mg SP/egg, 35 mg SP/egg. In experiment 1, in ovo injection of SP (1.5–3.5 mg/egg) increased hatchability of chicks (p < .01). In the groups received 2.5 and 3.5 mg SP/egg the expression of HSP70 and GPx genes was lower and higher than control groups, respectively (p < .001). The expression of interferon-gamma (IFN-γ) in the chicks consumed 1.5–3.5 mg SP/egg was higher than control groups (p < .001). The groups received 1.5–3.5 mg SP had lower feed conversion ratio (FCR, p < .05). Catalase activity in hatchlings consumed 2.5 or 3.5 mg SP was the highest (p < .05). In experiment 2, in ovo injection of 25 or 35 mg SP caused the lowest HSP70 in chicks (p < .0001). During the whole period of rearing, feed intake (FI) and FCR of the broilers in ovo fed with 35 mg SP was higher than control groups (p < .01). SP can be considered as an organic supplement in hatcheries for improving hatchability and anti-oxidant status of quail and broiler hatchlings.Highlights Spirulina platensis have the potential to be considered as a functional organic supplement in commercial hatcheries. In ovo injection of Spirulina platensis can improve hatchability, antioxidant, and immunity-related gene expression in quail chicks. In ovo injection of Spirulina platensis has positive effect on heat tolerance of broiler embryo during last days of incubation.http://dx.doi.org/10.1080/1828051X.2021.1910582gpxhsp70in ovoifn-γspirulina |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hosna Hajati Mojtaba Zaghari Omid Noori Reza Negarandeh Haniel Cedraz de Oliveira |
spellingShingle |
Hosna Hajati Mojtaba Zaghari Omid Noori Reza Negarandeh Haniel Cedraz de Oliveira Effects of in ovo injection of microalgae on hatchability, antioxidant and immunity-related genes expression, and post-hatch performance in broilers and Japanese quails Italian Journal of Animal Science gpx hsp70 in ovo ifn-γ spirulina |
author_facet |
Hosna Hajati Mojtaba Zaghari Omid Noori Reza Negarandeh Haniel Cedraz de Oliveira |
author_sort |
Hosna Hajati |
title |
Effects of in ovo injection of microalgae on hatchability, antioxidant and immunity-related genes expression, and post-hatch performance in broilers and Japanese quails |
title_short |
Effects of in ovo injection of microalgae on hatchability, antioxidant and immunity-related genes expression, and post-hatch performance in broilers and Japanese quails |
title_full |
Effects of in ovo injection of microalgae on hatchability, antioxidant and immunity-related genes expression, and post-hatch performance in broilers and Japanese quails |
title_fullStr |
Effects of in ovo injection of microalgae on hatchability, antioxidant and immunity-related genes expression, and post-hatch performance in broilers and Japanese quails |
title_full_unstemmed |
Effects of in ovo injection of microalgae on hatchability, antioxidant and immunity-related genes expression, and post-hatch performance in broilers and Japanese quails |
title_sort |
effects of in ovo injection of microalgae on hatchability, antioxidant and immunity-related genes expression, and post-hatch performance in broilers and japanese quails |
publisher |
Taylor & Francis Group |
series |
Italian Journal of Animal Science |
issn |
1594-4077 1828-051X |
publishDate |
2021-01-01 |
description |
The effects of in ovo injection of Arthrospira (Spirulina) Platensis on hatchability, antioxidant and immunity-related genes expression, and hatchling performance of broilers and Japanese quails was assessed in 2 separate experiments. In experiment 1, a total of 240 fertilised quail eggs were divided into six groups: control (without injection), sham control (0.02 CC distilled water/egg), 0.75 mg Spirulina Platensis (SP)/egg, 1.5 mg SP/egg, 2.5 mg SP/egg, 3.5 mg SP/egg. In experiment 2, a total of 192 fertilised broiler breeder were divided into four groups: control, sham control (0.2 CC distilled water/egg), 25 mg SP/egg, 35 mg SP/egg. In experiment 1, in ovo injection of SP (1.5–3.5 mg/egg) increased hatchability of chicks (p < .01). In the groups received 2.5 and 3.5 mg SP/egg the expression of HSP70 and GPx genes was lower and higher than control groups, respectively (p < .001). The expression of interferon-gamma (IFN-γ) in the chicks consumed 1.5–3.5 mg SP/egg was higher than control groups (p < .001). The groups received 1.5–3.5 mg SP had lower feed conversion ratio (FCR, p < .05). Catalase activity in hatchlings consumed 2.5 or 3.5 mg SP was the highest (p < .05). In experiment 2, in ovo injection of 25 or 35 mg SP caused the lowest HSP70 in chicks (p < .0001). During the whole period of rearing, feed intake (FI) and FCR of the broilers in ovo fed with 35 mg SP was higher than control groups (p < .01). SP can be considered as an organic supplement in hatcheries for improving hatchability and anti-oxidant status of quail and broiler hatchlings.Highlights Spirulina platensis have the potential to be considered as a functional organic supplement in commercial hatcheries. In ovo injection of Spirulina platensis can improve hatchability, antioxidant, and immunity-related gene expression in quail chicks. In ovo injection of Spirulina platensis has positive effect on heat tolerance of broiler embryo during last days of incubation. |
topic |
gpx hsp70 in ovo ifn-γ spirulina |
url |
http://dx.doi.org/10.1080/1828051X.2021.1910582 |
work_keys_str_mv |
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