Antibody-Based Protective Immunity against Helminth Infections: Antibody Phage Display Derived Antibodies against BmR1 Antigen
Helminth parasite infections are significantly impacting global health, with more than two billion infections worldwide with a high morbidity rate. The complex life cycle of the nematodes has made host immune response studies against these parasites extremely difficult. In this study, we utilized tw...
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doaj-6042743cde1846ad93244378dc79c9dc2020-11-25T00:29:48ZengMDPI AGInternational Journal of Molecular Sciences1422-00672017-11-011811237610.3390/ijms18112376ijms18112376Antibody-Based Protective Immunity against Helminth Infections: Antibody Phage Display Derived Antibodies against BmR1 AntigenAnizah Rahumatullah0Izzati Zahidah Abdul Karim1Rahmah Noordin2Theam Soon Lim3Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Minden 11800, Penang, MalaysiaInstitute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Minden 11800, Penang, MalaysiaInstitute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Minden 11800, Penang, MalaysiaInstitute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Minden 11800, Penang, MalaysiaHelminth parasite infections are significantly impacting global health, with more than two billion infections worldwide with a high morbidity rate. The complex life cycle of the nematodes has made host immune response studies against these parasites extremely difficult. In this study, we utilized two phage antibody libraries; the immune and naïve library were used to identify single chain fragment variable (scFv) clones against a specific filarial antigen (BmR1). The V-gene analysis of isolated scFv clones will help shed light on preferential VDJ gene segment usage against the filarial BmR1 antigen in healthy and infected states. The immune library showed the usage of both lambda and kappa light chains. However, the naïve library showed preferential use of the lambda family with different amino acid distributions. The binding characteristics of the scFv clones identified from this work were analyzed by immunoassay and immunoaffinity pull down of BmR1. The work highlights the antibody gene usage pattern of a naïve and immune antibody library against the same antigen as well as the robust nature of the enriched antibodies for downstream applications.https://www.mdpi.com/1422-0067/18/11/2376single chain fragment variable (scFv)monoclonal antibodiesBmR1phage displayimmune librarynaïve library |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anizah Rahumatullah Izzati Zahidah Abdul Karim Rahmah Noordin Theam Soon Lim |
spellingShingle |
Anizah Rahumatullah Izzati Zahidah Abdul Karim Rahmah Noordin Theam Soon Lim Antibody-Based Protective Immunity against Helminth Infections: Antibody Phage Display Derived Antibodies against BmR1 Antigen International Journal of Molecular Sciences single chain fragment variable (scFv) monoclonal antibodies BmR1 phage display immune library naïve library |
author_facet |
Anizah Rahumatullah Izzati Zahidah Abdul Karim Rahmah Noordin Theam Soon Lim |
author_sort |
Anizah Rahumatullah |
title |
Antibody-Based Protective Immunity against Helminth Infections: Antibody Phage Display Derived Antibodies against BmR1 Antigen |
title_short |
Antibody-Based Protective Immunity against Helminth Infections: Antibody Phage Display Derived Antibodies against BmR1 Antigen |
title_full |
Antibody-Based Protective Immunity against Helminth Infections: Antibody Phage Display Derived Antibodies against BmR1 Antigen |
title_fullStr |
Antibody-Based Protective Immunity against Helminth Infections: Antibody Phage Display Derived Antibodies against BmR1 Antigen |
title_full_unstemmed |
Antibody-Based Protective Immunity against Helminth Infections: Antibody Phage Display Derived Antibodies against BmR1 Antigen |
title_sort |
antibody-based protective immunity against helminth infections: antibody phage display derived antibodies against bmr1 antigen |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2017-11-01 |
description |
Helminth parasite infections are significantly impacting global health, with more than two billion infections worldwide with a high morbidity rate. The complex life cycle of the nematodes has made host immune response studies against these parasites extremely difficult. In this study, we utilized two phage antibody libraries; the immune and naïve library were used to identify single chain fragment variable (scFv) clones against a specific filarial antigen (BmR1). The V-gene analysis of isolated scFv clones will help shed light on preferential VDJ gene segment usage against the filarial BmR1 antigen in healthy and infected states. The immune library showed the usage of both lambda and kappa light chains. However, the naïve library showed preferential use of the lambda family with different amino acid distributions. The binding characteristics of the scFv clones identified from this work were analyzed by immunoassay and immunoaffinity pull down of BmR1. The work highlights the antibody gene usage pattern of a naïve and immune antibody library against the same antigen as well as the robust nature of the enriched antibodies for downstream applications. |
topic |
single chain fragment variable (scFv) monoclonal antibodies BmR1 phage display immune library naïve library |
url |
https://www.mdpi.com/1422-0067/18/11/2376 |
work_keys_str_mv |
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