Maternal dietary omega-3 fatty acid intake increases resolvin and protectin levels in the rat placenta
Placental inflammation is associated with several pregnancy disorders. Inflammation is limited by anti-inflammatory and proresolving mechanisms, the latter partly mediated by resolvins and protectins derived from omega-3 polyunsaturated fatty acids (n-3PUFA). We examined effects of dietary n-3PUFAs...
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doaj-6047a9708a1148739dd177bb2b930cf02021-04-28T06:02:15ZengElsevierJournal of Lipid Research0022-22752013-08-0154822472254Maternal dietary omega-3 fatty acid intake increases resolvin and protectin levels in the rat placentaMegan L. Jones0Peter J. Mark1Jeffrey A. Keelan2Anne Barden3Emilie Mas4Trevor A. Mori5Brendan J. Waddell6Schools of Anatomy, Physiology & Human Biology, The University of Western Australia, Perth, Western Australia 6009, AustraliaSchools of Anatomy, Physiology & Human Biology, The University of Western Australia, Perth, Western Australia 6009, AustraliaWomen's & Infants’ Health, and The University of Western Australia, Perth, Western Australia 6009, AustraliaMedicine & Pharmacology, The University of Western Australia, Perth, Western Australia 6009, AustraliaMedicine & Pharmacology, The University of Western Australia, Perth, Western Australia 6009, AustraliaMedicine & Pharmacology, The University of Western Australia, Perth, Western Australia 6009, AustraliaTo whom correspondence should be addressed; Schools of Anatomy, Physiology & Human Biology, The University of Western Australia, Perth, Western Australia 6009, AustraliaPlacental inflammation is associated with several pregnancy disorders. Inflammation is limited by anti-inflammatory and proresolving mechanisms, the latter partly mediated by resolvins and protectins derived from omega-3 polyunsaturated fatty acids (n-3PUFA). We examined effects of dietary n-3PUFAs on levels of resolvins, protectins, and lipoxygenase (ALOX) enzymes in the rat placenta. Rats consumed standard (Std) or high n-3PUFA (Hn3) diets from day 1 of pregnancy; tissues were collected on day 17 or 22 (term = day 23). Maternal Hn3 diet increased resolvin and protectin precursors, 18R/S-HEPE (P < 0.001), and 17R/S-HDHA (P < 0.01) at both days. Resolvins (17R-RvD1 and RvD1) increased at day 22 (P < 0.001) after Hn3 consumption, coincident with higher Alox15b and Alox5 mRNA expression, while RvD2 increased at both days (P < 0.05). Protectins, PD1, and 10S,17S-DiHDHA increased over late gestation (P < 0.001), coincident with higher Alox15 mRNA expression (P < 0.001) and further increased with Hn3 diet (P < 0.05). Maternal systemic and placental proinflammatory mediators were not suppressed by Hn3 diet; systemic IL1β, placental Il1β, and Il6 mRNA expression increased marginally with Hn3 at day 22 (P < 0.001), while Ptgs1 (Cox1) expression increased both days (P < 0.05). Our data indicate that maternal n-3PUFA supplementation enhances expression of enzymes in the n-3PUFA metabolic pathway and increases placental levels of resolvins and protectins.http://www.sciencedirect.com/science/article/pii/S0022227520375404n-3PUFAinflammationpregnancylipoxygenase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Megan L. Jones Peter J. Mark Jeffrey A. Keelan Anne Barden Emilie Mas Trevor A. Mori Brendan J. Waddell |
spellingShingle |
Megan L. Jones Peter J. Mark Jeffrey A. Keelan Anne Barden Emilie Mas Trevor A. Mori Brendan J. Waddell Maternal dietary omega-3 fatty acid intake increases resolvin and protectin levels in the rat placenta Journal of Lipid Research n-3PUFA inflammation pregnancy lipoxygenase |
author_facet |
Megan L. Jones Peter J. Mark Jeffrey A. Keelan Anne Barden Emilie Mas Trevor A. Mori Brendan J. Waddell |
author_sort |
Megan L. Jones |
title |
Maternal dietary omega-3 fatty acid intake increases resolvin and protectin levels in the rat placenta |
title_short |
Maternal dietary omega-3 fatty acid intake increases resolvin and protectin levels in the rat placenta |
title_full |
Maternal dietary omega-3 fatty acid intake increases resolvin and protectin levels in the rat placenta |
title_fullStr |
Maternal dietary omega-3 fatty acid intake increases resolvin and protectin levels in the rat placenta |
title_full_unstemmed |
Maternal dietary omega-3 fatty acid intake increases resolvin and protectin levels in the rat placenta |
title_sort |
maternal dietary omega-3 fatty acid intake increases resolvin and protectin levels in the rat placenta |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2013-08-01 |
description |
Placental inflammation is associated with several pregnancy disorders. Inflammation is limited by anti-inflammatory and proresolving mechanisms, the latter partly mediated by resolvins and protectins derived from omega-3 polyunsaturated fatty acids (n-3PUFA). We examined effects of dietary n-3PUFAs on levels of resolvins, protectins, and lipoxygenase (ALOX) enzymes in the rat placenta. Rats consumed standard (Std) or high n-3PUFA (Hn3) diets from day 1 of pregnancy; tissues were collected on day 17 or 22 (term = day 23). Maternal Hn3 diet increased resolvin and protectin precursors, 18R/S-HEPE (P < 0.001), and 17R/S-HDHA (P < 0.01) at both days. Resolvins (17R-RvD1 and RvD1) increased at day 22 (P < 0.001) after Hn3 consumption, coincident with higher Alox15b and Alox5 mRNA expression, while RvD2 increased at both days (P < 0.05). Protectins, PD1, and 10S,17S-DiHDHA increased over late gestation (P < 0.001), coincident with higher Alox15 mRNA expression (P < 0.001) and further increased with Hn3 diet (P < 0.05). Maternal systemic and placental proinflammatory mediators were not suppressed by Hn3 diet; systemic IL1β, placental Il1β, and Il6 mRNA expression increased marginally with Hn3 at day 22 (P < 0.001), while Ptgs1 (Cox1) expression increased both days (P < 0.05). Our data indicate that maternal n-3PUFA supplementation enhances expression of enzymes in the n-3PUFA metabolic pathway and increases placental levels of resolvins and protectins. |
topic |
n-3PUFA inflammation pregnancy lipoxygenase |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520375404 |
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