Comparison of pharmacokinetics of different oral Panax notoginseng saponins using ultra-high performance liquid mass spectrometry
Objective: To discuss and compare the plasma pharmacokinetics after three oral Panax notoginseng saponin (PNS) administrations in beagle dogs. PNS is the main active ingredient of the traditional Chinese medicine (TCM) Panax notoginseng. Although its outstanding therapeutic efficacy has been demonst...
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doaj-604d000f55154359b8fabdce50ccd0ef2021-04-02T19:12:56ZengElsevierJournal of Traditional Chinese Medical Sciences2095-75482021-01-01819098Comparison of pharmacokinetics of different oral Panax notoginseng saponins using ultra-high performance liquid mass spectrometryHuichao Wu0Huimin Liu1Shouyin Du2Jie Bai3Yang Lu4Lin Zhang5School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, ChinaDepartment of Pharmacy, Wuhan Hospital of Traditional Chinese Medicine, Wuhan 430000, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China; Corresponding author.School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, ChinaObjective: To discuss and compare the plasma pharmacokinetics after three oral Panax notoginseng saponin (PNS) administrations in beagle dogs. PNS is the main active ingredient of the traditional Chinese medicine (TCM) Panax notoginseng. Although its outstanding therapeutic efficacy has been demonstrated by various researchers, its broader application is restricted by the low bioavailability of PNS. Methods: An ultra-high performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous quantification of notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1, ginsenoside Rd, and ginsenoside Re in beagle dog plasma was developed and validated. The plasma samples were subjected to liquid–liquid extraction with acetone and methanol, and separated on an ACQUITY C18 column (100 × 2.1 mm ID, 1.7 μm) using acetonitrile and water as the mobile phase with a run time of 4.5 min. Results: The analytes were detected without interference in Selected Reaction Monitoring mode with positive electrospray ionization. The validated method was successfully used in comparative pharmacokinetic studies of the five saponins in beagle dogs after oral administration of three PNS preparations. Blood samples were collected up to 192 h after administration and pharmacokinetic parameters were calculated using DAS 3.20 and SPSS 17.0. The AUC0–t values of Re and R1 were significantly higher in soft capsules than in hard capsules. However, the AUC0–t values between hard and soft capsules were not significantly different for the other three components—Rb1, Rd and Rg1. Conclusion: Our intuitive analysis suggests that the bioavailability of PNS in soft capsules is greater than in hard capsules.http://www.sciencedirect.com/science/article/pii/S2095754817301151Ultra-high performance liquid mass spectrometryPanax notoginseng saponin preparationSoft capsuleHard capsuleComparison of pharmacokinetics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huichao Wu Huimin Liu Shouyin Du Jie Bai Yang Lu Lin Zhang |
spellingShingle |
Huichao Wu Huimin Liu Shouyin Du Jie Bai Yang Lu Lin Zhang Comparison of pharmacokinetics of different oral Panax notoginseng saponins using ultra-high performance liquid mass spectrometry Journal of Traditional Chinese Medical Sciences Ultra-high performance liquid mass spectrometry Panax notoginseng saponin preparation Soft capsule Hard capsule Comparison of pharmacokinetics |
author_facet |
Huichao Wu Huimin Liu Shouyin Du Jie Bai Yang Lu Lin Zhang |
author_sort |
Huichao Wu |
title |
Comparison of pharmacokinetics of different oral Panax notoginseng saponins using ultra-high performance liquid mass spectrometry |
title_short |
Comparison of pharmacokinetics of different oral Panax notoginseng saponins using ultra-high performance liquid mass spectrometry |
title_full |
Comparison of pharmacokinetics of different oral Panax notoginseng saponins using ultra-high performance liquid mass spectrometry |
title_fullStr |
Comparison of pharmacokinetics of different oral Panax notoginseng saponins using ultra-high performance liquid mass spectrometry |
title_full_unstemmed |
Comparison of pharmacokinetics of different oral Panax notoginseng saponins using ultra-high performance liquid mass spectrometry |
title_sort |
comparison of pharmacokinetics of different oral panax notoginseng saponins using ultra-high performance liquid mass spectrometry |
publisher |
Elsevier |
series |
Journal of Traditional Chinese Medical Sciences |
issn |
2095-7548 |
publishDate |
2021-01-01 |
description |
Objective: To discuss and compare the plasma pharmacokinetics after three oral Panax notoginseng saponin (PNS) administrations in beagle dogs. PNS is the main active ingredient of the traditional Chinese medicine (TCM) Panax notoginseng. Although its outstanding therapeutic efficacy has been demonstrated by various researchers, its broader application is restricted by the low bioavailability of PNS. Methods: An ultra-high performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous quantification of notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1, ginsenoside Rd, and ginsenoside Re in beagle dog plasma was developed and validated. The plasma samples were subjected to liquid–liquid extraction with acetone and methanol, and separated on an ACQUITY C18 column (100 × 2.1 mm ID, 1.7 μm) using acetonitrile and water as the mobile phase with a run time of 4.5 min. Results: The analytes were detected without interference in Selected Reaction Monitoring mode with positive electrospray ionization. The validated method was successfully used in comparative pharmacokinetic studies of the five saponins in beagle dogs after oral administration of three PNS preparations. Blood samples were collected up to 192 h after administration and pharmacokinetic parameters were calculated using DAS 3.20 and SPSS 17.0. The AUC0–t values of Re and R1 were significantly higher in soft capsules than in hard capsules. However, the AUC0–t values between hard and soft capsules were not significantly different for the other three components—Rb1, Rd and Rg1. Conclusion: Our intuitive analysis suggests that the bioavailability of PNS in soft capsules is greater than in hard capsules. |
topic |
Ultra-high performance liquid mass spectrometry Panax notoginseng saponin preparation Soft capsule Hard capsule Comparison of pharmacokinetics |
url |
http://www.sciencedirect.com/science/article/pii/S2095754817301151 |
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