Determination of luteolin from extracts of Helicteres hirsuta by HPLC

• Main Authors: Le Trung Hieu, Le Lam Son, Nguyen Minh Nhung, Ho Xuan Anh Vu, Tran Thi Anh Thi
• Format: Article
• Language: English
• Published: HUJOS 2019-07-01
• Series: Tạp chí Khoa học Đại học Huế: Khoa học Tự nhiên
• Subjects: luteolin; helicteres hirsuta; hplc
• Online Access: http://jos.hueuni.edu.vn/index.php/HUJOS-NS/article/view/5195

High-performance liquid chromatography coupled with a photodiode array detector (HPLC- DAD) has been reported to quantify isolated compounds. This work was designed, therefore, to develop an HPLC-DAD system to determine luteolin in the extract solutions from Helicteres hirsuta. Luteolin was analyzed on an RP-C18 column using a mobile phase including acetonitrile – 0.1% phosphoric acid (v/v) = 1:1 (v/v) with a detecting wavelength of 347 nm, a flow rate of 0.5 mL/min, and a volume of an injected sample of 10 μL. The HPLC system was carried out at ambient temperature. The method shows linearity for luteolin in the range 0.02–1 mg/mL, and the recovery of luteolin is 94.07 ± 0.64 %. This is the first time, the contents of luteolin in methanol extracts from the plant parts of H. hirsuta (including branch, fruit, and aerial parts) were determined with a value of 49.06 ± 0.46, 56.61 ± 0.62 and 91.15 ± .42 µg/g, respectively.