Extract and estimate the peroxidase enzyme from bean kales Phaseolus vulgaris L
Peroxidase enzyme was extracted from callus of different explants and immature embryos of Phaseolus vulgaris L. .The maximum activity of enzyme showed in the embryos callus sample.The higher activity of enzyme was in the ( 4months) age of callus for each of leaves ,roots and embryos and in (6months)...
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College of Education for Pure Sciences
2007-09-01
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doaj-605f09d9eab4448da942f822785b11b52020-11-25T02:13:09ZaraCollege of Education for Pure Sciencesمجلة التربية والعلم1812-125X2664-25302007-09-0120139240110.33899/edusj.2007.162819162819Extract and estimate the peroxidase enzyme from bean kales Phaseolus vulgaris LMra Al-KatebAbdullah Al-NuaimiPeroxidase enzyme was extracted from callus of different explants and immature embryos of Phaseolus vulgaris L. .The maximum activity of enzyme showed in the embryos callus sample.The higher activity of enzyme was in the ( 4months) age of callus for each of leaves ,roots and embryos and in (6months) for cotyledons callus,the stems callus recorded best activity in (2months)old.Using of ammonium sulphate precipitation result in high enzyme activity in all samples.When the exchange chromatochraphy was used to purifiy stems callus extract,(5)protein<br />apexes observed and ( 4 )of it was active .In the separation of proteins by using the electrofousing techniques, the samples of embryos ,leaves,stems,cotyledons and roots were separated at pH range (5.13- 6.14 ).The minimum activity of enzyme observed at ( 4day) age for all explants samples,but the maximum activity was in the roots samples at (14day)agehttps://edusj.mosuljournals.com/article_162819_1c0a6a3fc97a19fe59295cea243e899b.pdfenzymebean kalesphaseolus vulgaris l |
collection |
DOAJ |
language |
Arabic |
format |
Article |
sources |
DOAJ |
author |
Mra Al-Kateb Abdullah Al-Nuaimi |
spellingShingle |
Mra Al-Kateb Abdullah Al-Nuaimi Extract and estimate the peroxidase enzyme from bean kales Phaseolus vulgaris L مجلة التربية والعلم enzyme bean kales phaseolus vulgaris l |
author_facet |
Mra Al-Kateb Abdullah Al-Nuaimi |
author_sort |
Mra Al-Kateb |
title |
Extract and estimate the peroxidase enzyme from bean kales Phaseolus vulgaris L |
title_short |
Extract and estimate the peroxidase enzyme from bean kales Phaseolus vulgaris L |
title_full |
Extract and estimate the peroxidase enzyme from bean kales Phaseolus vulgaris L |
title_fullStr |
Extract and estimate the peroxidase enzyme from bean kales Phaseolus vulgaris L |
title_full_unstemmed |
Extract and estimate the peroxidase enzyme from bean kales Phaseolus vulgaris L |
title_sort |
extract and estimate the peroxidase enzyme from bean kales phaseolus vulgaris l |
publisher |
College of Education for Pure Sciences |
series |
مجلة التربية والعلم |
issn |
1812-125X 2664-2530 |
publishDate |
2007-09-01 |
description |
Peroxidase enzyme was extracted from callus of different explants and immature embryos of Phaseolus vulgaris L. .The maximum activity of enzyme showed in the embryos callus sample.The higher activity of enzyme was in the ( 4months) age of callus for each of leaves ,roots and embryos and in (6months) for cotyledons callus,the stems callus recorded best activity in (2months)old.Using of ammonium sulphate precipitation result in high enzyme activity in all samples.When the exchange chromatochraphy was used to purifiy stems callus extract,(5)protein<br />apexes observed and ( 4 )of it was active .In the separation of proteins by using the electrofousing techniques, the samples of embryos ,leaves,stems,cotyledons and roots were separated at pH range (5.13- 6.14 ).The minimum activity of enzyme observed at ( 4day) age for all explants samples,but the maximum activity was in the roots samples at (14day)age |
topic |
enzyme bean kales phaseolus vulgaris l |
url |
https://edusj.mosuljournals.com/article_162819_1c0a6a3fc97a19fe59295cea243e899b.pdf |
work_keys_str_mv |
AT mraalkateb extractandestimatetheperoxidaseenzymefrombeankalesphaseolusvulgarisl AT abdullahalnuaimi extractandestimatetheperoxidaseenzymefrombeankalesphaseolusvulgarisl |
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1724906075681980416 |