Growth, Immunity, and Transcriptome Response to Dietary Wnt/β-Catenin Pathway Activator TWS119 in Penaeus vannamei
Wnt/β-catenin signalling plays an essential role in the immunity of Penaeus vannamei. In this study, the effects of dietary Wnt/β-catenin pathway activator TWS119 on the growth, immunity, and transcriptome response in P. vannamei were investigated. Penaeus vannamei were fed diets with added TWS119 a...
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doaj-607f91b727c04e1b832697d8d4c5cb152021-06-04T07:43:40ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452021-06-01810.3389/fmars.2021.685429685429Growth, Immunity, and Transcriptome Response to Dietary Wnt/β-Catenin Pathway Activator TWS119 in Penaeus vannameiCuihong Hou0Shougang Song1Lulu Zhu2Lili Shi3Beiping Tan4Beiping Tan5Beiping Tan6Shuang Zhang7Shuang Zhang8Shuang Zhang9College of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaKey Laboratory of Aquatic Non-grain-based Feed Resources, Ministry of Agriculture, Zhanjiang, ChinaAquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaKey Laboratory of Aquatic Non-grain-based Feed Resources, Ministry of Agriculture, Zhanjiang, ChinaAquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, ChinaWnt/β-catenin signalling plays an essential role in the immunity of Penaeus vannamei. In this study, the effects of dietary Wnt/β-catenin pathway activator TWS119 on the growth, immunity, and transcriptome response in P. vannamei were investigated. Penaeus vannamei were fed diets with added TWS119 at doses of 0 (T0), 0.25 (T0.25), 1 (T1), 4 (T4), 16 (T16), or 64 mg·kg−1 (T64), respectively. LvGSK3β activity was effectively inhibited in P. vannamei given TWS119. The growth of P. vannamei in the T16 group was significantly improved when compared with the control group. After Vibrio parahaemolyticus infection, the survival rates (SRs) of P. vannamei in all experimental groups except the T64 group were significantly higher than in the T0 group. Compared with the control group, the immune enzymes’ activities in the serum of P. vannamei increased in all the experimental groups, while the malondialdehyde (MDA) contents decreased. Transcriptome analysis identified 5,073 differentially expressed genes (DEGs) for P. vannamei in the T0 and T16 groups. Most of the DEGs are involved in the ribosome pathway, endocytosis, glycerophospholipid metabolism, Wnt signalling, and FoxO =signalling pathways. The majority of the DEGs were from the ribosome pathway, which is also the most significantly enriched pathway. The study confirmed that the growth and immunity status of P. vannamei could improve by increasing dietary TWS119, which probably regulates the activity of the Wnt/β-catenin pathway and may be closely related to ribosome function and energy metabolism.https://www.frontiersin.org/articles/10.3389/fmars.2021.685429/fullPenaeus vannameiTWS119growth performanceimmunitytranscriptome analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cuihong Hou Shougang Song Lulu Zhu Lili Shi Beiping Tan Beiping Tan Beiping Tan Shuang Zhang Shuang Zhang Shuang Zhang |
spellingShingle |
Cuihong Hou Shougang Song Lulu Zhu Lili Shi Beiping Tan Beiping Tan Beiping Tan Shuang Zhang Shuang Zhang Shuang Zhang Growth, Immunity, and Transcriptome Response to Dietary Wnt/β-Catenin Pathway Activator TWS119 in Penaeus vannamei Frontiers in Marine Science Penaeus vannamei TWS119 growth performance immunity transcriptome analysis |
author_facet |
Cuihong Hou Shougang Song Lulu Zhu Lili Shi Beiping Tan Beiping Tan Beiping Tan Shuang Zhang Shuang Zhang Shuang Zhang |
author_sort |
Cuihong Hou |
title |
Growth, Immunity, and Transcriptome Response to Dietary Wnt/β-Catenin Pathway Activator TWS119 in Penaeus vannamei |
title_short |
Growth, Immunity, and Transcriptome Response to Dietary Wnt/β-Catenin Pathway Activator TWS119 in Penaeus vannamei |
title_full |
Growth, Immunity, and Transcriptome Response to Dietary Wnt/β-Catenin Pathway Activator TWS119 in Penaeus vannamei |
title_fullStr |
Growth, Immunity, and Transcriptome Response to Dietary Wnt/β-Catenin Pathway Activator TWS119 in Penaeus vannamei |
title_full_unstemmed |
Growth, Immunity, and Transcriptome Response to Dietary Wnt/β-Catenin Pathway Activator TWS119 in Penaeus vannamei |
title_sort |
growth, immunity, and transcriptome response to dietary wnt/β-catenin pathway activator tws119 in penaeus vannamei |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Marine Science |
issn |
2296-7745 |
publishDate |
2021-06-01 |
description |
Wnt/β-catenin signalling plays an essential role in the immunity of Penaeus vannamei. In this study, the effects of dietary Wnt/β-catenin pathway activator TWS119 on the growth, immunity, and transcriptome response in P. vannamei were investigated. Penaeus vannamei were fed diets with added TWS119 at doses of 0 (T0), 0.25 (T0.25), 1 (T1), 4 (T4), 16 (T16), or 64 mg·kg−1 (T64), respectively. LvGSK3β activity was effectively inhibited in P. vannamei given TWS119. The growth of P. vannamei in the T16 group was significantly improved when compared with the control group. After Vibrio parahaemolyticus infection, the survival rates (SRs) of P. vannamei in all experimental groups except the T64 group were significantly higher than in the T0 group. Compared with the control group, the immune enzymes’ activities in the serum of P. vannamei increased in all the experimental groups, while the malondialdehyde (MDA) contents decreased. Transcriptome analysis identified 5,073 differentially expressed genes (DEGs) for P. vannamei in the T0 and T16 groups. Most of the DEGs are involved in the ribosome pathway, endocytosis, glycerophospholipid metabolism, Wnt signalling, and FoxO =signalling pathways. The majority of the DEGs were from the ribosome pathway, which is also the most significantly enriched pathway. The study confirmed that the growth and immunity status of P. vannamei could improve by increasing dietary TWS119, which probably regulates the activity of the Wnt/β-catenin pathway and may be closely related to ribosome function and energy metabolism. |
topic |
Penaeus vannamei TWS119 growth performance immunity transcriptome analysis |
url |
https://www.frontiersin.org/articles/10.3389/fmars.2021.685429/full |
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