Growth, Immunity, and Transcriptome Response to Dietary Wnt/β-Catenin Pathway Activator TWS119 in Penaeus vannamei

Wnt/β-catenin signalling plays an essential role in the immunity of Penaeus vannamei. In this study, the effects of dietary Wnt/β-catenin pathway activator TWS119 on the growth, immunity, and transcriptome response in P. vannamei were investigated. Penaeus vannamei were fed diets with added TWS119 a...

Full description

Bibliographic Details
Main Authors: Cuihong Hou, Shougang Song, Lulu Zhu, Lili Shi, Beiping Tan, Shuang Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-06-01
Series:Frontiers in Marine Science
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fmars.2021.685429/full
id doaj-607f91b727c04e1b832697d8d4c5cb15
record_format Article
spelling doaj-607f91b727c04e1b832697d8d4c5cb152021-06-04T07:43:40ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452021-06-01810.3389/fmars.2021.685429685429Growth, Immunity, and Transcriptome Response to Dietary Wnt/β-Catenin Pathway Activator TWS119 in Penaeus vannameiCuihong Hou0Shougang Song1Lulu Zhu2Lili Shi3Beiping Tan4Beiping Tan5Beiping Tan6Shuang Zhang7Shuang Zhang8Shuang Zhang9College of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaKey Laboratory of Aquatic Non-grain-based Feed Resources, Ministry of Agriculture, Zhanjiang, ChinaAquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, ChinaCollege of Fisheries, Guangdong Ocean University, Zhanjiang, ChinaKey Laboratory of Aquatic Non-grain-based Feed Resources, Ministry of Agriculture, Zhanjiang, ChinaAquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, ChinaWnt/β-catenin signalling plays an essential role in the immunity of Penaeus vannamei. In this study, the effects of dietary Wnt/β-catenin pathway activator TWS119 on the growth, immunity, and transcriptome response in P. vannamei were investigated. Penaeus vannamei were fed diets with added TWS119 at doses of 0 (T0), 0.25 (T0.25), 1 (T1), 4 (T4), 16 (T16), or 64 mg·kg−1 (T64), respectively. LvGSK3β activity was effectively inhibited in P. vannamei given TWS119. The growth of P. vannamei in the T16 group was significantly improved when compared with the control group. After Vibrio parahaemolyticus infection, the survival rates (SRs) of P. vannamei in all experimental groups except the T64 group were significantly higher than in the T0 group. Compared with the control group, the immune enzymes’ activities in the serum of P. vannamei increased in all the experimental groups, while the malondialdehyde (MDA) contents decreased. Transcriptome analysis identified 5,073 differentially expressed genes (DEGs) for P. vannamei in the T0 and T16 groups. Most of the DEGs are involved in the ribosome pathway, endocytosis, glycerophospholipid metabolism, Wnt signalling, and FoxO =signalling pathways. The majority of the DEGs were from the ribosome pathway, which is also the most significantly enriched pathway. The study confirmed that the growth and immunity status of P. vannamei could improve by increasing dietary TWS119, which probably regulates the activity of the Wnt/β-catenin pathway and may be closely related to ribosome function and energy metabolism.https://www.frontiersin.org/articles/10.3389/fmars.2021.685429/fullPenaeus vannameiTWS119growth performanceimmunitytranscriptome analysis
collection DOAJ
language English
format Article
sources DOAJ
author Cuihong Hou
Shougang Song
Lulu Zhu
Lili Shi
Beiping Tan
Beiping Tan
Beiping Tan
Shuang Zhang
Shuang Zhang
Shuang Zhang
spellingShingle Cuihong Hou
Shougang Song
Lulu Zhu
Lili Shi
Beiping Tan
Beiping Tan
Beiping Tan
Shuang Zhang
Shuang Zhang
Shuang Zhang
Growth, Immunity, and Transcriptome Response to Dietary Wnt/β-Catenin Pathway Activator TWS119 in Penaeus vannamei
Frontiers in Marine Science
Penaeus vannamei
TWS119
growth performance
immunity
transcriptome analysis
author_facet Cuihong Hou
Shougang Song
Lulu Zhu
Lili Shi
Beiping Tan
Beiping Tan
Beiping Tan
Shuang Zhang
Shuang Zhang
Shuang Zhang
author_sort Cuihong Hou
title Growth, Immunity, and Transcriptome Response to Dietary Wnt/β-Catenin Pathway Activator TWS119 in Penaeus vannamei
title_short Growth, Immunity, and Transcriptome Response to Dietary Wnt/β-Catenin Pathway Activator TWS119 in Penaeus vannamei
title_full Growth, Immunity, and Transcriptome Response to Dietary Wnt/β-Catenin Pathway Activator TWS119 in Penaeus vannamei
title_fullStr Growth, Immunity, and Transcriptome Response to Dietary Wnt/β-Catenin Pathway Activator TWS119 in Penaeus vannamei
title_full_unstemmed Growth, Immunity, and Transcriptome Response to Dietary Wnt/β-Catenin Pathway Activator TWS119 in Penaeus vannamei
title_sort growth, immunity, and transcriptome response to dietary wnt/β-catenin pathway activator tws119 in penaeus vannamei
publisher Frontiers Media S.A.
series Frontiers in Marine Science
issn 2296-7745
publishDate 2021-06-01
description Wnt/β-catenin signalling plays an essential role in the immunity of Penaeus vannamei. In this study, the effects of dietary Wnt/β-catenin pathway activator TWS119 on the growth, immunity, and transcriptome response in P. vannamei were investigated. Penaeus vannamei were fed diets with added TWS119 at doses of 0 (T0), 0.25 (T0.25), 1 (T1), 4 (T4), 16 (T16), or 64 mg·kg−1 (T64), respectively. LvGSK3β activity was effectively inhibited in P. vannamei given TWS119. The growth of P. vannamei in the T16 group was significantly improved when compared with the control group. After Vibrio parahaemolyticus infection, the survival rates (SRs) of P. vannamei in all experimental groups except the T64 group were significantly higher than in the T0 group. Compared with the control group, the immune enzymes’ activities in the serum of P. vannamei increased in all the experimental groups, while the malondialdehyde (MDA) contents decreased. Transcriptome analysis identified 5,073 differentially expressed genes (DEGs) for P. vannamei in the T0 and T16 groups. Most of the DEGs are involved in the ribosome pathway, endocytosis, glycerophospholipid metabolism, Wnt signalling, and FoxO =signalling pathways. The majority of the DEGs were from the ribosome pathway, which is also the most significantly enriched pathway. The study confirmed that the growth and immunity status of P. vannamei could improve by increasing dietary TWS119, which probably regulates the activity of the Wnt/β-catenin pathway and may be closely related to ribosome function and energy metabolism.
topic Penaeus vannamei
TWS119
growth performance
immunity
transcriptome analysis
url https://www.frontiersin.org/articles/10.3389/fmars.2021.685429/full
work_keys_str_mv AT cuihonghou growthimmunityandtranscriptomeresponsetodietarywntbcateninpathwayactivatortws119inpenaeusvannamei
AT shougangsong growthimmunityandtranscriptomeresponsetodietarywntbcateninpathwayactivatortws119inpenaeusvannamei
AT luluzhu growthimmunityandtranscriptomeresponsetodietarywntbcateninpathwayactivatortws119inpenaeusvannamei
AT lilishi growthimmunityandtranscriptomeresponsetodietarywntbcateninpathwayactivatortws119inpenaeusvannamei
AT beipingtan growthimmunityandtranscriptomeresponsetodietarywntbcateninpathwayactivatortws119inpenaeusvannamei
AT beipingtan growthimmunityandtranscriptomeresponsetodietarywntbcateninpathwayactivatortws119inpenaeusvannamei
AT beipingtan growthimmunityandtranscriptomeresponsetodietarywntbcateninpathwayactivatortws119inpenaeusvannamei
AT shuangzhang growthimmunityandtranscriptomeresponsetodietarywntbcateninpathwayactivatortws119inpenaeusvannamei
AT shuangzhang growthimmunityandtranscriptomeresponsetodietarywntbcateninpathwayactivatortws119inpenaeusvannamei
AT shuangzhang growthimmunityandtranscriptomeresponsetodietarywntbcateninpathwayactivatortws119inpenaeusvannamei
_version_ 1721398129603903488