Isolation and selection of a strain of bacillus subtilis group for high antagonistic activity against Colletotrichum scovilleicausing chilli anthracnose disease in Ho Chi Minh City

• Main Authors: Trần Thùy Trang, Nguyễn Thị Ánh Nguyệt, Lê Thị Mai Châm, Nguyễn Tấn Đức, Phạm Nguyễn Đức Hoàng, Dương Hoa Xô
• Format: Article
• Language: English
• Published: HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE 2020-11-01
• Series: Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ
• Subjects: bacillus subtilis; bhcm8.3; colletotrichum scovillei; thán thư trên ớt
• Online Access: https://journalofscience.ou.edu.vn/index.php/tech-vi/article/view/1022
• ISSN: 2734-9322; 2734-9594

Chilli anthracnose disease caused by Colletotrichum spp. has heavily damaged the quality and yield production of chili around the world. In Viet Nam, many chili growing regions namely Ho Chi Minh City have been enormously affected by the disease for many years. Nowadays, a biological control using antagonistic microorganisms to prevent plant pathogens is becoming increasingly popular due to its safety and effectiveness. In particular, bacteria belonging to Bacillus subtilis group has been proven to have antagonistic ability against pathogenic fungi. Therefore, this study was conducted to isolate and select bacteria of Bacillus subtilis group which show high antagonistic activity against the fungus Colletotrichum scovillei causing Chilli anthracnose disease in Ho Chi Minh City. From five soil samples, the study isolated 22 candidate strains that initially categorized as Bacillus subtilis group. Out of 22 isolates, the BHCM8.3 strain showed the best inhibitory effect on the growth of Colletotrichum scovillei in the dual-culture agar overlay method (antagonistic effectiveness is 81.58% after 15 days). 16S ribosomal DNA (rDNA)-based molecular identification reveals that the BHCM8.3 strain is completely identical to the bacterium Bacillus subtilis (100%).