24(S)-Saringosterol Prevents Cognitive Decline in a Mouse Model for Alzheimer’s Disease
We recently found that dietary supplementation with the seaweed <i>Sargassum fusiforme</i>, containing the preferential LXRβ-agonist 24(S)-saringosterol, prevented memory decline and reduced amyloid-β (Aβ) deposition in an Alzheimer’s disease (AD) mouse model without inducing hepatic ste...
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Format: | Article |
Language: | English |
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MDPI AG
2021-03-01
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Series: | Marine Drugs |
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Online Access: | https://www.mdpi.com/1660-3397/19/4/190 |
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doaj-60ab9b769a024bbc9a50fbfe5a1ccf2c |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nikita Martens Melissa Schepers Na Zhan Frank Leijten Gardi Voortman Assia Tiane Ben Rombaut Janne Poisquet Nienke van de Sande Anja Kerksiek Folkert Kuipers Johan W. Jonker Hongbing Liu Dieter Lütjohann Tim Vanmierlo Monique T. Mulder |
spellingShingle |
Nikita Martens Melissa Schepers Na Zhan Frank Leijten Gardi Voortman Assia Tiane Ben Rombaut Janne Poisquet Nienke van de Sande Anja Kerksiek Folkert Kuipers Johan W. Jonker Hongbing Liu Dieter Lütjohann Tim Vanmierlo Monique T. Mulder 24(S)-Saringosterol Prevents Cognitive Decline in a Mouse Model for Alzheimer’s Disease Marine Drugs Alzheimer’s disease seaweed <i>Sargassum fusiforme</i> phytosterols cholesterol metabolism |
author_facet |
Nikita Martens Melissa Schepers Na Zhan Frank Leijten Gardi Voortman Assia Tiane Ben Rombaut Janne Poisquet Nienke van de Sande Anja Kerksiek Folkert Kuipers Johan W. Jonker Hongbing Liu Dieter Lütjohann Tim Vanmierlo Monique T. Mulder |
author_sort |
Nikita Martens |
title |
24(S)-Saringosterol Prevents Cognitive Decline in a Mouse Model for Alzheimer’s Disease |
title_short |
24(S)-Saringosterol Prevents Cognitive Decline in a Mouse Model for Alzheimer’s Disease |
title_full |
24(S)-Saringosterol Prevents Cognitive Decline in a Mouse Model for Alzheimer’s Disease |
title_fullStr |
24(S)-Saringosterol Prevents Cognitive Decline in a Mouse Model for Alzheimer’s Disease |
title_full_unstemmed |
24(S)-Saringosterol Prevents Cognitive Decline in a Mouse Model for Alzheimer’s Disease |
title_sort |
24(s)-saringosterol prevents cognitive decline in a mouse model for alzheimer’s disease |
publisher |
MDPI AG |
series |
Marine Drugs |
issn |
1660-3397 |
publishDate |
2021-03-01 |
description |
We recently found that dietary supplementation with the seaweed <i>Sargassum fusiforme</i>, containing the preferential LXRβ-agonist 24(S)-saringosterol, prevented memory decline and reduced amyloid-β (Aβ) deposition in an Alzheimer’s disease (AD) mouse model without inducing hepatic steatosis. Here, we examined the effects of 24(S)-saringosterol as a food additive on cognition and neuropathology in AD mice. Six-month-old male APPswePS1ΔE9 mice and wildtype C57BL/6J littermates received 24(S)-saringosterol (0.5 mg/25 g body weight/day) (APPswePS1ΔE9 <i>n</i> = 20; C57BL/6J <i>n</i> = 19) or vehicle (APPswePS1ΔE9 <i>n</i> = 17; C57BL/6J <i>n</i> = 19) for 10 weeks. Cognition was assessed using object recognition and object location tasks. Sterols were analyzed by gas chromatography/mass spectrometry, Aβ and inflammatory markers by immunohistochemistry, and gene expression by quantitative real-time PCR. Hepatic lipids were quantified after Oil-Red-O staining. Administration of 24(S)-saringosterol prevented cognitive decline in APPswePS1ΔE9 mice without affecting the Aβ plaque load. Moreover, 24(S)-saringosterol prevented the increase in the inflammatory marker Iba1 in the cortex of APPswePS1ΔE9 mice (<i>p</i> < 0.001). Furthermore, 24(S)-saringosterol did not affect the expression of lipid metabolism-related LXR-response genes in the hippocampus nor the hepatic neutral lipid content. Thus, administration of 24(S)-saringosterol prevented cognitive decline in APPswePS1ΔE9 mice independent of effects on Aβ load and without adverse effects on liver fat content. The anti-inflammatory effects of 24(S)-saringosterol may contribute to the prevention of cognitive decline. |
topic |
Alzheimer’s disease seaweed <i>Sargassum fusiforme</i> phytosterols cholesterol metabolism |
url |
https://www.mdpi.com/1660-3397/19/4/190 |
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doaj-60ab9b769a024bbc9a50fbfe5a1ccf2c2021-03-28T00:02:30ZengMDPI AGMarine Drugs1660-33972021-03-011919019010.3390/md1904019024(S)-Saringosterol Prevents Cognitive Decline in a Mouse Model for Alzheimer’s DiseaseNikita Martens0Melissa Schepers1Na Zhan2Frank Leijten3Gardi Voortman4Assia Tiane5Ben Rombaut6Janne Poisquet7Nienke van de Sande8Anja Kerksiek9Folkert Kuipers10Johan W. Jonker11Hongbing Liu12Dieter Lütjohann13Tim Vanmierlo14Monique T. Mulder15Department of Internal Medicine, Section Pharmacology and Vascular Medicine, Erasmus University Medical Center, 3015CN Rotterdam, The NetherlandsDepartment of Neuroscience, Biomedical Research Institute, European Graduate School of Neuroscience, Hasselt University, Hasselt BE 3590, BelgiumDepartment of Internal Medicine, Section Pharmacology and Vascular Medicine, Erasmus University Medical Center, 3015CN Rotterdam, The NetherlandsDepartment of Internal Medicine, Section Pharmacology and Vascular Medicine, Erasmus University Medical Center, 3015CN Rotterdam, The NetherlandsDepartment of Internal Medicine, Section Pharmacology and Vascular Medicine, Erasmus University Medical Center, 3015CN Rotterdam, The NetherlandsDepartment of Neuroscience, Biomedical Research Institute, European Graduate School of Neuroscience, Hasselt University, Hasselt BE 3590, BelgiumDepartment of Neuroscience, Biomedical Research Institute, European Graduate School of Neuroscience, Hasselt University, Hasselt BE 3590, BelgiumDepartment of Neuroscience, Biomedical Research Institute, European Graduate School of Neuroscience, Hasselt University, Hasselt BE 3590, BelgiumDepartment of Internal Medicine, Section Pharmacology and Vascular Medicine, Erasmus University Medical Center, 3015CN Rotterdam, The NetherlandsInstitute of Clinical Chemistry and Clinical Pharmacology, University Hospital Bonn, D-53127 Bonn, GermanyDepartment of Pediatrics, Section of Molecular Metabolism and Nutrition, University Medical Center Groningen, 1205 Groningen, The NetherlandsDepartment of Pediatrics, Section of Molecular Metabolism and Nutrition, University Medical Center Groningen, 1205 Groningen, The NetherlandsKey Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, ChinaInstitute of Clinical Chemistry and Clinical Pharmacology, University Hospital Bonn, D-53127 Bonn, GermanyDepartment of Internal Medicine, Section Pharmacology and Vascular Medicine, Erasmus University Medical Center, 3015CN Rotterdam, The NetherlandsDepartment of Internal Medicine, Section Pharmacology and Vascular Medicine, Erasmus University Medical Center, 3015CN Rotterdam, The NetherlandsWe recently found that dietary supplementation with the seaweed <i>Sargassum fusiforme</i>, containing the preferential LXRβ-agonist 24(S)-saringosterol, prevented memory decline and reduced amyloid-β (Aβ) deposition in an Alzheimer’s disease (AD) mouse model without inducing hepatic steatosis. Here, we examined the effects of 24(S)-saringosterol as a food additive on cognition and neuropathology in AD mice. Six-month-old male APPswePS1ΔE9 mice and wildtype C57BL/6J littermates received 24(S)-saringosterol (0.5 mg/25 g body weight/day) (APPswePS1ΔE9 <i>n</i> = 20; C57BL/6J <i>n</i> = 19) or vehicle (APPswePS1ΔE9 <i>n</i> = 17; C57BL/6J <i>n</i> = 19) for 10 weeks. Cognition was assessed using object recognition and object location tasks. Sterols were analyzed by gas chromatography/mass spectrometry, Aβ and inflammatory markers by immunohistochemistry, and gene expression by quantitative real-time PCR. Hepatic lipids were quantified after Oil-Red-O staining. Administration of 24(S)-saringosterol prevented cognitive decline in APPswePS1ΔE9 mice without affecting the Aβ plaque load. Moreover, 24(S)-saringosterol prevented the increase in the inflammatory marker Iba1 in the cortex of APPswePS1ΔE9 mice (<i>p</i> < 0.001). Furthermore, 24(S)-saringosterol did not affect the expression of lipid metabolism-related LXR-response genes in the hippocampus nor the hepatic neutral lipid content. Thus, administration of 24(S)-saringosterol prevented cognitive decline in APPswePS1ΔE9 mice independent of effects on Aβ load and without adverse effects on liver fat content. The anti-inflammatory effects of 24(S)-saringosterol may contribute to the prevention of cognitive decline.https://www.mdpi.com/1660-3397/19/4/190Alzheimer’s diseaseseaweed<i>Sargassum fusiforme</i>phytosterolscholesterol metabolism |