Qualitative and quantitative analysis of chemicals emitted from the pheromone gland of individual Heliothis subflexa females.
The chemicals emitted from the sex pheromone gland of individual Heliothis subflexa females were sampled using a short section of thick-film megabore fused silica capillary column, and the pheromone glands of the same females were extracted after the effluvia collection. Both samples were treated wi...
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doaj-60b1a1448f73413f88ea1ef8b11ee1f22020-11-25T01:14:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01138e020203510.1371/journal.pone.0202035Qualitative and quantitative analysis of chemicals emitted from the pheromone gland of individual Heliothis subflexa females.Satoshi NojimaAlice ClassenAstrid T GrootCoby SchalThe chemicals emitted from the sex pheromone gland of individual Heliothis subflexa females were sampled using a short section of thick-film megabore fused silica capillary column, and the pheromone glands of the same females were extracted after the effluvia collection. Both samples were treated with a silylation reagent, and then subjected to gas chromatography-chemical ionization-mass spectrometry for quantitative and qualitative analysis of all components. The total amount of all 11 components emitted from the glands of calling females was 153 ng/female/hr, which was substantially higher than previously reported. The ratios of the pheromone components in the volatile emissions and pheromone gland extracts were generally similar to previous studies, but with notable differences. The collections of volatiles and gland extractions contained, respectively: Z9-14:Ald (1.57%, 1.35%), 14:Ald (3.78%, 1.51%), Z7 + Z9-16:Ald (9.60%, 3.59%), Z11-16:Ald (76.14%, 18.94%), 16:Ald (2.95%, 2.17%), Z9-16:OH (0.07%, 7.21%), Z11-16:OH (1.11%, 49.04%), Z7-16:OAc (0.48%, 1.73%), Z9-16:OAc (1.32%, 4.02%), and Z11-16:OAc (2.98%, 10.43%). The thick-film megabore column is an efficient approach for sampling the headspace for semiochemicals.http://europepmc.org/articles/PMC6091922?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Satoshi Nojima Alice Classen Astrid T Groot Coby Schal |
spellingShingle |
Satoshi Nojima Alice Classen Astrid T Groot Coby Schal Qualitative and quantitative analysis of chemicals emitted from the pheromone gland of individual Heliothis subflexa females. PLoS ONE |
author_facet |
Satoshi Nojima Alice Classen Astrid T Groot Coby Schal |
author_sort |
Satoshi Nojima |
title |
Qualitative and quantitative analysis of chemicals emitted from the pheromone gland of individual Heliothis subflexa females. |
title_short |
Qualitative and quantitative analysis of chemicals emitted from the pheromone gland of individual Heliothis subflexa females. |
title_full |
Qualitative and quantitative analysis of chemicals emitted from the pheromone gland of individual Heliothis subflexa females. |
title_fullStr |
Qualitative and quantitative analysis of chemicals emitted from the pheromone gland of individual Heliothis subflexa females. |
title_full_unstemmed |
Qualitative and quantitative analysis of chemicals emitted from the pheromone gland of individual Heliothis subflexa females. |
title_sort |
qualitative and quantitative analysis of chemicals emitted from the pheromone gland of individual heliothis subflexa females. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2018-01-01 |
description |
The chemicals emitted from the sex pheromone gland of individual Heliothis subflexa females were sampled using a short section of thick-film megabore fused silica capillary column, and the pheromone glands of the same females were extracted after the effluvia collection. Both samples were treated with a silylation reagent, and then subjected to gas chromatography-chemical ionization-mass spectrometry for quantitative and qualitative analysis of all components. The total amount of all 11 components emitted from the glands of calling females was 153 ng/female/hr, which was substantially higher than previously reported. The ratios of the pheromone components in the volatile emissions and pheromone gland extracts were generally similar to previous studies, but with notable differences. The collections of volatiles and gland extractions contained, respectively: Z9-14:Ald (1.57%, 1.35%), 14:Ald (3.78%, 1.51%), Z7 + Z9-16:Ald (9.60%, 3.59%), Z11-16:Ald (76.14%, 18.94%), 16:Ald (2.95%, 2.17%), Z9-16:OH (0.07%, 7.21%), Z11-16:OH (1.11%, 49.04%), Z7-16:OAc (0.48%, 1.73%), Z9-16:OAc (1.32%, 4.02%), and Z11-16:OAc (2.98%, 10.43%). The thick-film megabore column is an efficient approach for sampling the headspace for semiochemicals. |
url |
http://europepmc.org/articles/PMC6091922?pdf=render |
work_keys_str_mv |
AT satoshinojima qualitativeandquantitativeanalysisofchemicalsemittedfromthepheromoneglandofindividualheliothissubflexafemales AT aliceclassen qualitativeandquantitativeanalysisofchemicalsemittedfromthepheromoneglandofindividualheliothissubflexafemales AT astridtgroot qualitativeandquantitativeanalysisofchemicalsemittedfromthepheromoneglandofindividualheliothissubflexafemales AT cobyschal qualitativeandquantitativeanalysisofchemicalsemittedfromthepheromoneglandofindividualheliothissubflexafemales |
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