Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes

Summary: Multiple strategies have been developed to efficiently differentiate human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Here, we describe a protocol for measuring three key functional parameters of hiPSC-CMs, including contractile function, calcium (Ca2+) handling, and...

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Main Authors:	Joe Z. Zhang, Shane Rui Zhao, Chengyi Tu, Paul Pang, Mao Zhang, Joseph C. Wu
Format:	Article
Language:	English
Published:	Elsevier 2021-12-01
Series:	STAR Protocols
Subjects:	Cell Biology Cell culture Microscopy Stem Cells
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166721005657

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spelling	doaj-60bfd3d12128435f955f55e4666a200e2021-10-09T04:41:33ZengElsevierSTAR Protocols2666-16672021-12-0124100859Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytesJoe Z. Zhang0Shane Rui Zhao1Chengyi Tu2Paul Pang3Mao Zhang4Joseph C. Wu5Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA; Corresponding authorStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA; Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA; Corresponding authorSummary: Multiple strategies have been developed to efficiently differentiate human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Here, we describe a protocol for measuring three key functional parameters of hiPSC-CMs, including contractile function, calcium (Ca2+) handling, and action potential.For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166721005657Cell BiologyCell cultureMicroscopyStem Cells
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Joe Z. Zhang Shane Rui Zhao Chengyi Tu Paul Pang Mao Zhang Joseph C. Wu
spellingShingle	Joe Z. Zhang Shane Rui Zhao Chengyi Tu Paul Pang Mao Zhang Joseph C. Wu Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes STAR Protocols Cell Biology Cell culture Microscopy Stem Cells
author_facet	Joe Z. Zhang Shane Rui Zhao Chengyi Tu Paul Pang Mao Zhang Joseph C. Wu
author_sort	Joe Z. Zhang
title	Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes
title_short	Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes
title_full	Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes
title_fullStr	Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes
title_full_unstemmed	Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes
title_sort	protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes
publisher	Elsevier
series	STAR Protocols
issn	2666-1667
publishDate	2021-12-01
description	Summary: Multiple strategies have been developed to efficiently differentiate human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Here, we describe a protocol for measuring three key functional parameters of hiPSC-CMs, including contractile function, calcium (Ca2+) handling, and action potential.For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).
topic	Cell Biology Cell culture Microscopy Stem Cells
url	http://www.sciencedirect.com/science/article/pii/S2666166721005657
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Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes

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