Neurodegeneration and Sensorimotor Deficits in the Mouse Model of Traumatic Brain Injury
Traumatic brain injury (TBI) can result in persistent sensorimotor and cognitive deficits, which occur through a cascade of deleterious pathophysiological events over time. In this study, we investigated the hypothesis that neurodegeneration caused by TBI leads to impairments in sensorimotor functio...
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doaj-60c481dcaf4d4921bfaf74947a04f9082020-11-24T20:57:59ZengMDPI AGBrain Sciences2076-34252018-01-01811110.3390/brainsci8010011brainsci8010011Neurodegeneration and Sensorimotor Deficits in the Mouse Model of Traumatic Brain InjurySaurav Bhowmick0Veera D‘Mello1Nizmi Ponery2P. M. Abdul-Muneer3Laboratory of CNS Injury and Repair, Neuroscience Institute, JFK Medical Center, 65 James St, Edison, NJ 08820, USALaboratory of CNS Injury and Repair, Neuroscience Institute, JFK Medical Center, 65 James St, Edison, NJ 08820, USALaboratory of CNS Injury and Repair, Neuroscience Institute, JFK Medical Center, 65 James St, Edison, NJ 08820, USALaboratory of CNS Injury and Repair, Neuroscience Institute, JFK Medical Center, 65 James St, Edison, NJ 08820, USATraumatic brain injury (TBI) can result in persistent sensorimotor and cognitive deficits, which occur through a cascade of deleterious pathophysiological events over time. In this study, we investigated the hypothesis that neurodegeneration caused by TBI leads to impairments in sensorimotor function. TBI induces the activation of the caspase-3 enzyme, which triggers cell apoptosis in an in vivo model of fluid percussion injury (FPI). We analyzed caspase-3 mediated apoptosis by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining and poly (ADP-ribose) polymerase (PARP) and annexin V western blotting. We correlated the neurodegeneration with sensorimotor deficits by conducting the animal behavioral tests including grid walk, balance beam, the inverted screen test, and the climb test. Our study demonstrated that the excess cell death or neurodegeneration correlated with the neuronal dysfunction and sensorimotor impairments associated with TBI.http://www.mdpi.com/2076-3425/8/1/11traumatic brain injuryfluid percussion injuryneurodegenerationapoptosissensorimotor deficit |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Saurav Bhowmick Veera D‘Mello Nizmi Ponery P. M. Abdul-Muneer |
spellingShingle |
Saurav Bhowmick Veera D‘Mello Nizmi Ponery P. M. Abdul-Muneer Neurodegeneration and Sensorimotor Deficits in the Mouse Model of Traumatic Brain Injury Brain Sciences traumatic brain injury fluid percussion injury neurodegeneration apoptosis sensorimotor deficit |
author_facet |
Saurav Bhowmick Veera D‘Mello Nizmi Ponery P. M. Abdul-Muneer |
author_sort |
Saurav Bhowmick |
title |
Neurodegeneration and Sensorimotor Deficits in the Mouse Model of Traumatic Brain Injury |
title_short |
Neurodegeneration and Sensorimotor Deficits in the Mouse Model of Traumatic Brain Injury |
title_full |
Neurodegeneration and Sensorimotor Deficits in the Mouse Model of Traumatic Brain Injury |
title_fullStr |
Neurodegeneration and Sensorimotor Deficits in the Mouse Model of Traumatic Brain Injury |
title_full_unstemmed |
Neurodegeneration and Sensorimotor Deficits in the Mouse Model of Traumatic Brain Injury |
title_sort |
neurodegeneration and sensorimotor deficits in the mouse model of traumatic brain injury |
publisher |
MDPI AG |
series |
Brain Sciences |
issn |
2076-3425 |
publishDate |
2018-01-01 |
description |
Traumatic brain injury (TBI) can result in persistent sensorimotor and cognitive deficits, which occur through a cascade of deleterious pathophysiological events over time. In this study, we investigated the hypothesis that neurodegeneration caused by TBI leads to impairments in sensorimotor function. TBI induces the activation of the caspase-3 enzyme, which triggers cell apoptosis in an in vivo model of fluid percussion injury (FPI). We analyzed caspase-3 mediated apoptosis by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining and poly (ADP-ribose) polymerase (PARP) and annexin V western blotting. We correlated the neurodegeneration with sensorimotor deficits by conducting the animal behavioral tests including grid walk, balance beam, the inverted screen test, and the climb test. Our study demonstrated that the excess cell death or neurodegeneration correlated with the neuronal dysfunction and sensorimotor impairments associated with TBI. |
topic |
traumatic brain injury fluid percussion injury neurodegeneration apoptosis sensorimotor deficit |
url |
http://www.mdpi.com/2076-3425/8/1/11 |
work_keys_str_mv |
AT sauravbhowmick neurodegenerationandsensorimotordeficitsinthemousemodeloftraumaticbraininjury AT veeradmello neurodegenerationandsensorimotordeficitsinthemousemodeloftraumaticbraininjury AT nizmiponery neurodegenerationandsensorimotordeficitsinthemousemodeloftraumaticbraininjury AT pmabdulmuneer neurodegenerationandsensorimotordeficitsinthemousemodeloftraumaticbraininjury |
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1716786846107697152 |