Effect of Electroacupuncture on Gut Microbiota in Participants With Knee Osteoarthritis
A close relationship between knee osteoarthritis (KOA) and gut microbiota has recently been described. Herein, we aim to investigate the effect of electroacupuncture (EA) on gut microbiota in participants with KOA. We conducted a study of 60 participants with KOA and 30 matched healthy controls (HCs...
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Frontiers Media S.A.
2021-10-01
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Series: | Frontiers in Cellular and Infection Microbiology |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.597431/full |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tian-Qi Wang Ling-Ru Li Chun-Xia Tan Jing-Wen Yang Guang-Xia Shi Li-Qiong Wang Hui Hu Zhi-Shun Liu Jun Wang Tong Wang Yong Yuan Wen-Rui Jia Hua Li Xin-Wei Wang Bin Wu Jian-Feng Tu Cun-Zhi Liu |
spellingShingle |
Tian-Qi Wang Ling-Ru Li Chun-Xia Tan Jing-Wen Yang Guang-Xia Shi Li-Qiong Wang Hui Hu Zhi-Shun Liu Jun Wang Tong Wang Yong Yuan Wen-Rui Jia Hua Li Xin-Wei Wang Bin Wu Jian-Feng Tu Cun-Zhi Liu Effect of Electroacupuncture on Gut Microbiota in Participants With Knee Osteoarthritis Frontiers in Cellular and Infection Microbiology electroacupuncture gut microbiota knee osteoarthritis sham acupuncture effect |
author_facet |
Tian-Qi Wang Ling-Ru Li Chun-Xia Tan Jing-Wen Yang Guang-Xia Shi Li-Qiong Wang Hui Hu Zhi-Shun Liu Jun Wang Tong Wang Yong Yuan Wen-Rui Jia Hua Li Xin-Wei Wang Bin Wu Jian-Feng Tu Cun-Zhi Liu |
author_sort |
Tian-Qi Wang |
title |
Effect of Electroacupuncture on Gut Microbiota in Participants With Knee Osteoarthritis |
title_short |
Effect of Electroacupuncture on Gut Microbiota in Participants With Knee Osteoarthritis |
title_full |
Effect of Electroacupuncture on Gut Microbiota in Participants With Knee Osteoarthritis |
title_fullStr |
Effect of Electroacupuncture on Gut Microbiota in Participants With Knee Osteoarthritis |
title_full_unstemmed |
Effect of Electroacupuncture on Gut Microbiota in Participants With Knee Osteoarthritis |
title_sort |
effect of electroacupuncture on gut microbiota in participants with knee osteoarthritis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular and Infection Microbiology |
issn |
2235-2988 |
publishDate |
2021-10-01 |
description |
A close relationship between knee osteoarthritis (KOA) and gut microbiota has recently been described. Herein, we aim to investigate the effect of electroacupuncture (EA) on gut microbiota in participants with KOA. We conducted a study of 60 participants with KOA and 30 matched healthy controls (HCs). Sixty participants were allocated to either EA group (n=30) or sham acupuncture (SA) group (n=30). Five obligatory acupoints and three adjunct acupoints were punctured in the EA group. Eight non-acupoints that were separated from conventional acupoints or meridians were used for the SA group. Participants in both groups received 24 sessions within eight weeks. Fecal microbial analyses by 16S ribosomal RNA gene sequencing were carried out after collecting stools at T0 and T8 weeks (Four samples with changed defecation habits were excluded). The results showed that both Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) total score (P=0.043) and NRS score (P=0.002) decreased more in EA group than those in SA group. Moreover, EA could reverse more KOA-related bacteria including Bacteroides, [Eubacterium]_hallii_group, Agathobacter and Streptococcus. The number of significantly different genera between KOA patients and HCs were less after EA treatment than that after SA treatment. This meant that EA modified the composition of the gut microbiome, making it closer to healthy people, while not significantly affecting the microbial diversity. Two genera including Agathobacter (P=0.0163), Lachnoclostridium (P=0.0144) were statistically increased than baseline in EA group (paired Wilcoxon rank sum test). After EA treatment, Bacteroides (P=0.0394) was more abundant and Streptococcus (P=0.0306) was significantly reduced in patients who demonstrated adequate response than in those with inadequate response (Wilcoxon rank-sum test). Spearman correlation test between gut microbe and KOA clinical outcomes indicated that Bacteroides and Agathobacter was negatively correlated with NRS score, WOMAC total score, and WOMAC pain, stiffness and pain scores (P<0.001 or 0.05 or 0.01), while Streptococcus was positively correlated with them (P<0.05 or 0.01). Our study suggests that EA contributes to the improvement of KOA and gut microbiota could be a potential therapeutic target. |
topic |
electroacupuncture gut microbiota knee osteoarthritis sham acupuncture effect |
url |
https://www.frontiersin.org/articles/10.3389/fcimb.2021.597431/full |
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doaj-60d3f69ee4204e308ba2a9e8367834b52021-10-04T07:27:37ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-10-011110.3389/fcimb.2021.597431597431Effect of Electroacupuncture on Gut Microbiota in Participants With Knee OsteoarthritisTian-Qi Wang0Ling-Ru Li1Chun-Xia Tan2Jing-Wen Yang3Guang-Xia Shi4Li-Qiong Wang5Hui Hu6Zhi-Shun Liu7Jun Wang8Tong Wang9Yong Yuan10Wen-Rui Jia11Hua Li12Xin-Wei Wang13Bin Wu14Jian-Feng Tu15Cun-Zhi Liu16International Acupuncture and Moxibustion Innovation Institute, School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaNational Institute of Traditional Chinese Medicine Constitution and Preventive Treatment of Diseases, Beijing University of Chinese Medicine, Beijing, ChinaInternational Acupuncture and Moxibustion Innovation Institute, School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaInternational Acupuncture and Moxibustion Innovation Institute, School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaInternational Acupuncture and Moxibustion Innovation Institute, School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaInternational Acupuncture and Moxibustion Innovation Institute, School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Acupuncture and Moxibustion, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Acupuncture and Moxibustion, Guang’an Men Hospital, China Academy of Chinese Medical Sciences, Beijing, ChinaDepartment of Acupuncture and Moxibustion, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Orthopedics, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, ChinaDepartment of Orthopedics, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, ChinaInternational Acupuncture and Moxibustion Innovation Institute, School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Acupuncture and Moxibustion, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Acupuncture and Moxibustion, Guang’an Men Hospital, China Academy of Chinese Medical Sciences, Beijing, ChinaDepartment of Acupuncture and Moxibustion, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, ChinaInternational Acupuncture and Moxibustion Innovation Institute, School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaInternational Acupuncture and Moxibustion Innovation Institute, School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaA close relationship between knee osteoarthritis (KOA) and gut microbiota has recently been described. Herein, we aim to investigate the effect of electroacupuncture (EA) on gut microbiota in participants with KOA. We conducted a study of 60 participants with KOA and 30 matched healthy controls (HCs). Sixty participants were allocated to either EA group (n=30) or sham acupuncture (SA) group (n=30). Five obligatory acupoints and three adjunct acupoints were punctured in the EA group. Eight non-acupoints that were separated from conventional acupoints or meridians were used for the SA group. Participants in both groups received 24 sessions within eight weeks. Fecal microbial analyses by 16S ribosomal RNA gene sequencing were carried out after collecting stools at T0 and T8 weeks (Four samples with changed defecation habits were excluded). The results showed that both Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) total score (P=0.043) and NRS score (P=0.002) decreased more in EA group than those in SA group. Moreover, EA could reverse more KOA-related bacteria including Bacteroides, [Eubacterium]_hallii_group, Agathobacter and Streptococcus. The number of significantly different genera between KOA patients and HCs were less after EA treatment than that after SA treatment. This meant that EA modified the composition of the gut microbiome, making it closer to healthy people, while not significantly affecting the microbial diversity. Two genera including Agathobacter (P=0.0163), Lachnoclostridium (P=0.0144) were statistically increased than baseline in EA group (paired Wilcoxon rank sum test). After EA treatment, Bacteroides (P=0.0394) was more abundant and Streptococcus (P=0.0306) was significantly reduced in patients who demonstrated adequate response than in those with inadequate response (Wilcoxon rank-sum test). Spearman correlation test between gut microbe and KOA clinical outcomes indicated that Bacteroides and Agathobacter was negatively correlated with NRS score, WOMAC total score, and WOMAC pain, stiffness and pain scores (P<0.001 or 0.05 or 0.01), while Streptococcus was positively correlated with them (P<0.05 or 0.01). Our study suggests that EA contributes to the improvement of KOA and gut microbiota could be a potential therapeutic target.https://www.frontiersin.org/articles/10.3389/fcimb.2021.597431/fullelectroacupuncturegut microbiotaknee osteoarthritissham acupunctureeffect |