Molecular investigation of Cryptosporidium and Giardia in pre- and post-weaned calves in Hubei Province, China
Abstract Background The protistan pathogens Cryptosporidium and Giardia can cause significant intestinal diseases in animals and humans. Cattle, particularly calves, carrying these protists can be significant reservoirs for human infections and disease. However, little is known about the genetic mak...
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doaj-60df598f897044b9820cedb9aa6ee59d2020-11-25T02:17:56ZengBMCParasites & Vectors1756-33052017-10-011011710.1186/s13071-017-2463-3Molecular investigation of Cryptosporidium and Giardia in pre- and post-weaned calves in Hubei Province, ChinaYingying Fan0Tao Wang1Anson V. Koehler2Min Hu3Robin B. Gasser4State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural UniversityDepartment of Veterinary Biosciences, Melbourne Veterinary School, The University of MelbourneDepartment of Veterinary Biosciences, Melbourne Veterinary School, The University of MelbourneState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural UniversityState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural UniversityAbstract Background The protistan pathogens Cryptosporidium and Giardia can cause significant intestinal diseases in animals and humans. Cattle, particularly calves, carrying these protists can be significant reservoirs for human infections and disease. However, little is known about the genetic make-up of Cryptosporidium and Giardia populations in cattle and other ruminants in some regions of China. Results In the present study, PCR-based tools were used to genetically characterise these protists in faecal samples from a total of 339 pre- and post-weaned calves from four distinct locations in Hubei Province using markers in the large (LSU) or small (SSU) subunits of nuclear ribosomal RNA genes. Cryptosporidium andersoni, C. bovis, C. ryanae and Giardia duodenalis assemblage E were detected in 0.6%, 10.9%, 4.1% and 22.6% of calves, respectively. Conclusions This study is the first to report the prevalence of Cryptosporidium and Giardia in pre- and post-weaned calves in Hubei Province, and encourages large-scale molecular studies of animals and humans, in an effort to better understand the epidemiology of these enteric pathogens in China.http://link.springer.com/article/10.1186/s13071-017-2463-3CryptosporidiumGiardiaCalvesPCR-based sequencingNuclear ribosomal RNA genesChina |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yingying Fan Tao Wang Anson V. Koehler Min Hu Robin B. Gasser |
spellingShingle |
Yingying Fan Tao Wang Anson V. Koehler Min Hu Robin B. Gasser Molecular investigation of Cryptosporidium and Giardia in pre- and post-weaned calves in Hubei Province, China Parasites & Vectors Cryptosporidium Giardia Calves PCR-based sequencing Nuclear ribosomal RNA genes China |
author_facet |
Yingying Fan Tao Wang Anson V. Koehler Min Hu Robin B. Gasser |
author_sort |
Yingying Fan |
title |
Molecular investigation of Cryptosporidium and Giardia in pre- and post-weaned calves in Hubei Province, China |
title_short |
Molecular investigation of Cryptosporidium and Giardia in pre- and post-weaned calves in Hubei Province, China |
title_full |
Molecular investigation of Cryptosporidium and Giardia in pre- and post-weaned calves in Hubei Province, China |
title_fullStr |
Molecular investigation of Cryptosporidium and Giardia in pre- and post-weaned calves in Hubei Province, China |
title_full_unstemmed |
Molecular investigation of Cryptosporidium and Giardia in pre- and post-weaned calves in Hubei Province, China |
title_sort |
molecular investigation of cryptosporidium and giardia in pre- and post-weaned calves in hubei province, china |
publisher |
BMC |
series |
Parasites & Vectors |
issn |
1756-3305 |
publishDate |
2017-10-01 |
description |
Abstract Background The protistan pathogens Cryptosporidium and Giardia can cause significant intestinal diseases in animals and humans. Cattle, particularly calves, carrying these protists can be significant reservoirs for human infections and disease. However, little is known about the genetic make-up of Cryptosporidium and Giardia populations in cattle and other ruminants in some regions of China. Results In the present study, PCR-based tools were used to genetically characterise these protists in faecal samples from a total of 339 pre- and post-weaned calves from four distinct locations in Hubei Province using markers in the large (LSU) or small (SSU) subunits of nuclear ribosomal RNA genes. Cryptosporidium andersoni, C. bovis, C. ryanae and Giardia duodenalis assemblage E were detected in 0.6%, 10.9%, 4.1% and 22.6% of calves, respectively. Conclusions This study is the first to report the prevalence of Cryptosporidium and Giardia in pre- and post-weaned calves in Hubei Province, and encourages large-scale molecular studies of animals and humans, in an effort to better understand the epidemiology of these enteric pathogens in China. |
topic |
Cryptosporidium Giardia Calves PCR-based sequencing Nuclear ribosomal RNA genes China |
url |
http://link.springer.com/article/10.1186/s13071-017-2463-3 |
work_keys_str_mv |
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