Evaluation of antimicrobial resistance, biofilm forming potential, and the presence of biofilm-related genes among clinical isolates of Pseudomonas aeruginosa
Abstract Objectives Pseudomonas aeruginosa is known as a leading cause of nosocomial infections worldwide. Antimicrobial resistance and biofilm production, as two main virulence factors of P. aeruginosa, are responsible for the persistence of prolonged infections. In this study, antimicrobial suscep...
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doaj-60f6704cd3d54f46a0ad33f21e3ae1642021-01-10T12:51:22ZengBMCBMC Research Notes1756-05002020-01-011311610.1186/s13104-020-4890-zEvaluation of antimicrobial resistance, biofilm forming potential, and the presence of biofilm-related genes among clinical isolates of Pseudomonas aeruginosaEsmat Kamali0Ailar Jamali1Abdollah Ardebili2Freshteh Ezadi3Alireza Mohebbi4Laboratory Sciences Research Center, Golestan University of Medical SciencesLaboratory Sciences Research Center, Golestan University of Medical SciencesLaboratory Sciences Research Center, Golestan University of Medical SciencesLaboratory Sciences Research Center, Golestan University of Medical SciencesDepartment of Microbiology, Faculty of Medicine, Golestan University of Medical SciencesAbstract Objectives Pseudomonas aeruginosa is known as a leading cause of nosocomial infections worldwide. Antimicrobial resistance and biofilm production, as two main virulence factors of P. aeruginosa, are responsible for the persistence of prolonged infections. In this study, antimicrobial susceptibility pattern and phenotypic and genotypic characteristics of biofilm of P. aeruginosa were investigated. Results A total of 80 clinical P. aeruginosa isolates were obtained. Isolates showed resistance to all antibiotics with a rate from 12.5% (n = 10) against amikacin and piperacillin/tazobactam to 23.75% (n = 19) to levofloxacin. Multidrug-resistant P. aeruginosa accounted for 20% (n = 16). 83.75% (n = 67) of isolates showed biofilm phenotype. All three biofilm-related genes were found simultaneously in 87.5% (n = 70) of P. aeruginosa and 13.5% (n = 10) of the isolates had none of the genes tested. From the results of the present study, combination therapy including an anti-pseudomonal beta-lactam (piperacillin/tazobactam or ceftazidime) and an aminoglycoside or carbapenems (imipenem, meropenem) with fluoroquinolones in conjunction with an aminoglycoside can be used against Pseudomonas infections. However, reasonable antimicrobial use and high standards of infection prevention and control are essential to prevent further development of antimicrobial resistance. Combination strategies based on the proper anti-pseudomonal antibiotics along with anti-biofilm agents can also be selected to eradicate biofilm-associated infections.https://doi.org/10.1186/s13104-020-4890-zAntimicrobial resistanceBiofilm formationBiofilm genesPseudomonas aeruginosa |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Esmat Kamali Ailar Jamali Abdollah Ardebili Freshteh Ezadi Alireza Mohebbi |
spellingShingle |
Esmat Kamali Ailar Jamali Abdollah Ardebili Freshteh Ezadi Alireza Mohebbi Evaluation of antimicrobial resistance, biofilm forming potential, and the presence of biofilm-related genes among clinical isolates of Pseudomonas aeruginosa BMC Research Notes Antimicrobial resistance Biofilm formation Biofilm genes Pseudomonas aeruginosa |
author_facet |
Esmat Kamali Ailar Jamali Abdollah Ardebili Freshteh Ezadi Alireza Mohebbi |
author_sort |
Esmat Kamali |
title |
Evaluation of antimicrobial resistance, biofilm forming potential, and the presence of biofilm-related genes among clinical isolates of Pseudomonas aeruginosa |
title_short |
Evaluation of antimicrobial resistance, biofilm forming potential, and the presence of biofilm-related genes among clinical isolates of Pseudomonas aeruginosa |
title_full |
Evaluation of antimicrobial resistance, biofilm forming potential, and the presence of biofilm-related genes among clinical isolates of Pseudomonas aeruginosa |
title_fullStr |
Evaluation of antimicrobial resistance, biofilm forming potential, and the presence of biofilm-related genes among clinical isolates of Pseudomonas aeruginosa |
title_full_unstemmed |
Evaluation of antimicrobial resistance, biofilm forming potential, and the presence of biofilm-related genes among clinical isolates of Pseudomonas aeruginosa |
title_sort |
evaluation of antimicrobial resistance, biofilm forming potential, and the presence of biofilm-related genes among clinical isolates of pseudomonas aeruginosa |
publisher |
BMC |
series |
BMC Research Notes |
issn |
1756-0500 |
publishDate |
2020-01-01 |
description |
Abstract Objectives Pseudomonas aeruginosa is known as a leading cause of nosocomial infections worldwide. Antimicrobial resistance and biofilm production, as two main virulence factors of P. aeruginosa, are responsible for the persistence of prolonged infections. In this study, antimicrobial susceptibility pattern and phenotypic and genotypic characteristics of biofilm of P. aeruginosa were investigated. Results A total of 80 clinical P. aeruginosa isolates were obtained. Isolates showed resistance to all antibiotics with a rate from 12.5% (n = 10) against amikacin and piperacillin/tazobactam to 23.75% (n = 19) to levofloxacin. Multidrug-resistant P. aeruginosa accounted for 20% (n = 16). 83.75% (n = 67) of isolates showed biofilm phenotype. All three biofilm-related genes were found simultaneously in 87.5% (n = 70) of P. aeruginosa and 13.5% (n = 10) of the isolates had none of the genes tested. From the results of the present study, combination therapy including an anti-pseudomonal beta-lactam (piperacillin/tazobactam or ceftazidime) and an aminoglycoside or carbapenems (imipenem, meropenem) with fluoroquinolones in conjunction with an aminoglycoside can be used against Pseudomonas infections. However, reasonable antimicrobial use and high standards of infection prevention and control are essential to prevent further development of antimicrobial resistance. Combination strategies based on the proper anti-pseudomonal antibiotics along with anti-biofilm agents can also be selected to eradicate biofilm-associated infections. |
topic |
Antimicrobial resistance Biofilm formation Biofilm genes Pseudomonas aeruginosa |
url |
https://doi.org/10.1186/s13104-020-4890-z |
work_keys_str_mv |
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