Copper Oxide Nanoparticles Cause a Dose-Dependent Toxicity via Inducing Reactive Oxygen Species in Drosophila
Copper oxide nanoparticles (CuONPs) have attracted considerable attention, because of their biocide potential and capability for optical imaging, however CuONPs were shown to be highly toxic in various experimental model systems. In this study, mechanism underlying CuONP-induced toxicity was investi...
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doaj-60fe39275e0546c4a71d8112e7825e4d2020-11-24T21:10:33ZengMDPI AGNanomaterials2079-49912018-10-0181082410.3390/nano8100824nano8100824Copper Oxide Nanoparticles Cause a Dose-Dependent Toxicity via Inducing Reactive Oxygen Species in DrosophilaEugene Baeg0Kanidta Sooklert1Amornpun Sereemaspun2Daegu International School, 22 Palgongro, 50-Gil, Donggu, Daegu 701-170, KoreaNanomedicine Research Unit, Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandNanomedicine Research Unit, Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandCopper oxide nanoparticles (CuONPs) have attracted considerable attention, because of their biocide potential and capability for optical imaging, however CuONPs were shown to be highly toxic in various experimental model systems. In this study, mechanism underlying CuONP-induced toxicity was investigated using Drosophila as an in vivo model. Upon oral route of administration, CuONPs accumulated in the body, and caused a dose-dependent decrease in egg-to-adult survivorship and a delay in development. In particular, transmission electron microscopy analysis revealed CuONPs were detected inside the intestinal epithelial cells and lumen. A drastic increase in apoptosis and reactive oxygen species was also observed in the gut exposed to CuONPs. Importantly, we found that inhibition of the transcription factor Nrf2 further enhances the toxicity caused by CuONPs. These observations suggest that CuONPs disrupt the gut homeostasis and that oxidative stress serves as one of the primary causes of CuONP-induced toxicity in Drosophila.http://www.mdpi.com/2079-4991/8/10/824copper oxide nanoparticlecytotoxicityreactive oxygen speciesNrf2Drosophila melanogaster |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eugene Baeg Kanidta Sooklert Amornpun Sereemaspun |
spellingShingle |
Eugene Baeg Kanidta Sooklert Amornpun Sereemaspun Copper Oxide Nanoparticles Cause a Dose-Dependent Toxicity via Inducing Reactive Oxygen Species in Drosophila Nanomaterials copper oxide nanoparticle cytotoxicity reactive oxygen species Nrf2 Drosophila melanogaster |
author_facet |
Eugene Baeg Kanidta Sooklert Amornpun Sereemaspun |
author_sort |
Eugene Baeg |
title |
Copper Oxide Nanoparticles Cause a Dose-Dependent Toxicity via Inducing Reactive Oxygen Species in Drosophila |
title_short |
Copper Oxide Nanoparticles Cause a Dose-Dependent Toxicity via Inducing Reactive Oxygen Species in Drosophila |
title_full |
Copper Oxide Nanoparticles Cause a Dose-Dependent Toxicity via Inducing Reactive Oxygen Species in Drosophila |
title_fullStr |
Copper Oxide Nanoparticles Cause a Dose-Dependent Toxicity via Inducing Reactive Oxygen Species in Drosophila |
title_full_unstemmed |
Copper Oxide Nanoparticles Cause a Dose-Dependent Toxicity via Inducing Reactive Oxygen Species in Drosophila |
title_sort |
copper oxide nanoparticles cause a dose-dependent toxicity via inducing reactive oxygen species in drosophila |
publisher |
MDPI AG |
series |
Nanomaterials |
issn |
2079-4991 |
publishDate |
2018-10-01 |
description |
Copper oxide nanoparticles (CuONPs) have attracted considerable attention, because of their biocide potential and capability for optical imaging, however CuONPs were shown to be highly toxic in various experimental model systems. In this study, mechanism underlying CuONP-induced toxicity was investigated using Drosophila as an in vivo model. Upon oral route of administration, CuONPs accumulated in the body, and caused a dose-dependent decrease in egg-to-adult survivorship and a delay in development. In particular, transmission electron microscopy analysis revealed CuONPs were detected inside the intestinal epithelial cells and lumen. A drastic increase in apoptosis and reactive oxygen species was also observed in the gut exposed to CuONPs. Importantly, we found that inhibition of the transcription factor Nrf2 further enhances the toxicity caused by CuONPs. These observations suggest that CuONPs disrupt the gut homeostasis and that oxidative stress serves as one of the primary causes of CuONP-induced toxicity in Drosophila. |
topic |
copper oxide nanoparticle cytotoxicity reactive oxygen species Nrf2 Drosophila melanogaster |
url |
http://www.mdpi.com/2079-4991/8/10/824 |
work_keys_str_mv |
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1716756149129183232 |