Baicalein Rescues Delayed Cooling via Preservation of Akt Activation and Akt-Mediated Phospholamban Phosphorylation
Cooling reduces the ischemia/reperfusion (I/R) injury seen in sudden cardiac arrest (SCA) by decreasing the burst of reactive oxygen species (ROS). Its cardioprotection is diminished when delay in reaching the target temperature occurs. Baicalein, a flavonoid derived from the root of Scutellaria bai...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2018-03-01
|
Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | http://www.mdpi.com/1422-0067/19/4/973 |
id |
doaj-60ff566ed970449ab403ce9720bbdbd7 |
---|---|
record_format |
Article |
spelling |
doaj-60ff566ed970449ab403ce9720bbdbd72020-11-24T23:56:10ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-03-0119497310.3390/ijms19040973ijms19040973Baicalein Rescues Delayed Cooling via Preservation of Akt Activation and Akt-Mediated Phospholamban PhosphorylationZuohui Shao0Sy-Jou Chen1Xiangdong Zhu2Chunpei Lee3Hsien-Hao Huang4Angelo Meliton5Changqing Li6Terry L. Vanden Hoek7Jing Li8Center of Advanced Resuscitation Medicine, Center for Cardiovascular Research, Department of Emergency Medicine, University of Illinois Hospital, and Health Sciences System, Chicago, IL 60612, USACenter of Advanced Resuscitation Medicine, Center for Cardiovascular Research, Department of Emergency Medicine, University of Illinois Hospital, and Health Sciences System, Chicago, IL 60612, USACenter of Advanced Resuscitation Medicine, Center for Cardiovascular Research, Department of Emergency Medicine, University of Illinois Hospital, and Health Sciences System, Chicago, IL 60612, USACenter of Advanced Resuscitation Medicine, Center for Cardiovascular Research, Department of Emergency Medicine, University of Illinois Hospital, and Health Sciences System, Chicago, IL 60612, USACenter of Advanced Resuscitation Medicine, Center for Cardiovascular Research, Department of Emergency Medicine, University of Illinois Hospital, and Health Sciences System, Chicago, IL 60612, USADepartment of Medicine, Section of Pulmonary and Critical Care Medicine, University of Chicago, Chicago, IL 60637, USACenter of Advanced Resuscitation Medicine, Center for Cardiovascular Research, Department of Emergency Medicine, University of Illinois Hospital, and Health Sciences System, Chicago, IL 60612, USACenter of Advanced Resuscitation Medicine, Center for Cardiovascular Research, Department of Emergency Medicine, University of Illinois Hospital, and Health Sciences System, Chicago, IL 60612, USACenter of Advanced Resuscitation Medicine, Center for Cardiovascular Research, Department of Emergency Medicine, University of Illinois Hospital, and Health Sciences System, Chicago, IL 60612, USACooling reduces the ischemia/reperfusion (I/R) injury seen in sudden cardiac arrest (SCA) by decreasing the burst of reactive oxygen species (ROS). Its cardioprotection is diminished when delay in reaching the target temperature occurs. Baicalein, a flavonoid derived from the root of Scutellaria baicalensis Georgi, possesses antioxidant properties. Therefore, we hypothesized that baicalein can rescue cooling cardioprotection when cooling is delayed. Two murine cardiomyocyte models, an I/R model (90 min ischemia/3 h reperfusion) and stunning model (30 min ischemia/90 min reperfusion), were used to assess cell survival and contractility, respectively. Cooling (32 °C) was initiated either during ischemia or during reperfusion. Cell viability and ROS generation were measured. Cell contractility was evaluated by real-time phase-contrast imaging. Our results showed that cooling reduced cell death and ROS generation, and this effect was diminished when cooling was delayed. Baicalein (25 µM), given either at the start of reperfusion or start of cooling, resulted in a comparable reduction of cell death and ROS production. Baicalein improved phospholamban phosphorylation, contractility recovery, and cell survival. These effects were Akt-dependent. In addition, no synergistic effect was observed with the combined treatments of cooling and baicalein. Our data suggest that baicalein may serve as a novel adjunct therapeutic strategy for SCA resuscitation.http://www.mdpi.com/1422-0067/19/4/973intraischemic coolingdelayed coolingbaicaleinischemia/reperfusionreactive oxygen species (ROS)cardiomyocyteAktcell deathcontractility |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zuohui Shao Sy-Jou Chen Xiangdong Zhu Chunpei Lee Hsien-Hao Huang Angelo Meliton Changqing Li Terry L. Vanden Hoek Jing Li |
spellingShingle |
Zuohui Shao Sy-Jou Chen Xiangdong Zhu Chunpei Lee Hsien-Hao Huang Angelo Meliton Changqing Li Terry L. Vanden Hoek Jing Li Baicalein Rescues Delayed Cooling via Preservation of Akt Activation and Akt-Mediated Phospholamban Phosphorylation International Journal of Molecular Sciences intraischemic cooling delayed cooling baicalein ischemia/reperfusion reactive oxygen species (ROS) cardiomyocyte Akt cell death contractility |
author_facet |
Zuohui Shao Sy-Jou Chen Xiangdong Zhu Chunpei Lee Hsien-Hao Huang Angelo Meliton Changqing Li Terry L. Vanden Hoek Jing Li |
author_sort |
Zuohui Shao |
title |
Baicalein Rescues Delayed Cooling via Preservation of Akt Activation and Akt-Mediated Phospholamban Phosphorylation |
title_short |
Baicalein Rescues Delayed Cooling via Preservation of Akt Activation and Akt-Mediated Phospholamban Phosphorylation |
title_full |
Baicalein Rescues Delayed Cooling via Preservation of Akt Activation and Akt-Mediated Phospholamban Phosphorylation |
title_fullStr |
Baicalein Rescues Delayed Cooling via Preservation of Akt Activation and Akt-Mediated Phospholamban Phosphorylation |
title_full_unstemmed |
Baicalein Rescues Delayed Cooling via Preservation of Akt Activation and Akt-Mediated Phospholamban Phosphorylation |
title_sort |
baicalein rescues delayed cooling via preservation of akt activation and akt-mediated phospholamban phosphorylation |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2018-03-01 |
description |
Cooling reduces the ischemia/reperfusion (I/R) injury seen in sudden cardiac arrest (SCA) by decreasing the burst of reactive oxygen species (ROS). Its cardioprotection is diminished when delay in reaching the target temperature occurs. Baicalein, a flavonoid derived from the root of Scutellaria baicalensis Georgi, possesses antioxidant properties. Therefore, we hypothesized that baicalein can rescue cooling cardioprotection when cooling is delayed. Two murine cardiomyocyte models, an I/R model (90 min ischemia/3 h reperfusion) and stunning model (30 min ischemia/90 min reperfusion), were used to assess cell survival and contractility, respectively. Cooling (32 °C) was initiated either during ischemia or during reperfusion. Cell viability and ROS generation were measured. Cell contractility was evaluated by real-time phase-contrast imaging. Our results showed that cooling reduced cell death and ROS generation, and this effect was diminished when cooling was delayed. Baicalein (25 µM), given either at the start of reperfusion or start of cooling, resulted in a comparable reduction of cell death and ROS production. Baicalein improved phospholamban phosphorylation, contractility recovery, and cell survival. These effects were Akt-dependent. In addition, no synergistic effect was observed with the combined treatments of cooling and baicalein. Our data suggest that baicalein may serve as a novel adjunct therapeutic strategy for SCA resuscitation. |
topic |
intraischemic cooling delayed cooling baicalein ischemia/reperfusion reactive oxygen species (ROS) cardiomyocyte Akt cell death contractility |
url |
http://www.mdpi.com/1422-0067/19/4/973 |
work_keys_str_mv |
AT zuohuishao baicaleinrescuesdelayedcoolingviapreservationofaktactivationandaktmediatedphospholambanphosphorylation AT syjouchen baicaleinrescuesdelayedcoolingviapreservationofaktactivationandaktmediatedphospholambanphosphorylation AT xiangdongzhu baicaleinrescuesdelayedcoolingviapreservationofaktactivationandaktmediatedphospholambanphosphorylation AT chunpeilee baicaleinrescuesdelayedcoolingviapreservationofaktactivationandaktmediatedphospholambanphosphorylation AT hsienhaohuang baicaleinrescuesdelayedcoolingviapreservationofaktactivationandaktmediatedphospholambanphosphorylation AT angelomeliton baicaleinrescuesdelayedcoolingviapreservationofaktactivationandaktmediatedphospholambanphosphorylation AT changqingli baicaleinrescuesdelayedcoolingviapreservationofaktactivationandaktmediatedphospholambanphosphorylation AT terrylvandenhoek baicaleinrescuesdelayedcoolingviapreservationofaktactivationandaktmediatedphospholambanphosphorylation AT jingli baicaleinrescuesdelayedcoolingviapreservationofaktactivationandaktmediatedphospholambanphosphorylation |
_version_ |
1725459297622556672 |