A New Approach for the Fabrication of Cytocompatible PLLA-Magnetite Nanoparticle Composite Scaffolds

Magnetic biomimetic scaffolds of poly(L-lactide) (PLLA) and nanoparticles of magnetite (nFe<sub>3</sub>O<sub>4</sub>) are prepared in a wide ratio of compositions by lyophilization for bone regeneration. The magnetic properties, cytotoxicity, and the in vitro degradation of t...

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Main Authors:	Esperanza Díaz, María Blanca Valle, Sylvie Ribeiro, Senentxu Lanceros‑Mendez, José Manuel Barandiarán
Format:	Article
Language:	English
Published:	MDPI AG 2019-09-01
Series:	International Journal of Molecular Sciences
Subjects:	polymer scission PLLA magnetite cytotoxicity magnetism in vitro degradation
Online Access:	https://www.mdpi.com/1422-0067/20/19/4664

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spelling	doaj-6101e93115f44e9893065b2bcf6697b82020-11-25T02:08:00ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-09-012019466410.3390/ijms20194664ijms20194664A New Approach for the Fabrication of Cytocompatible PLLA-Magnetite Nanoparticle Composite ScaffoldsEsperanza Díaz0María Blanca Valle1Sylvie Ribeiro2Senentxu Lanceros‑Mendez3José Manuel Barandiarán4Escuela de Ingeniería de Bilbao, Departamento de Ingeniería Minera, Metalúrgica y Ciencia de Materiales, Universidad del País Vasco (UPV/EHU), 48920 Portugalete, SpainFacultad de Ciencia y Tecnología, Departamento de Electricidad y Electrónica, University of the Basque Country (UPV/EHU), Sarriena s/n, 48940 Leioa, SpainCentro de Física, Universidade do Minho, 4710-057 Braga, PortugalBCMaterials, Basque Centre for Materials, Applications and Nanostructures, UPV/EHU Science Park, 48940 Leioa, SpainBCMaterials, Basque Centre for Materials, Applications and Nanostructures, UPV/EHU Science Park, 48940 Leioa, SpainMagnetic biomimetic scaffolds of poly(L-lactide) (PLLA) and nanoparticles of magnetite (nFe<sub>3</sub>O<sub>4</sub>) are prepared in a wide ratio of compositions by lyophilization for bone regeneration. The magnetic properties, cytotoxicity, and the in vitro degradation of these porous materials are closely studied. The addition of magnetite at 50 °C was found to produce an interaction reaction between the ester groups of the PLLA and the metallic cations of the magnetite, causing the formation of complexes. This fact was confirmed by the analysis of the infrared spectroscopy and the gel permeation chromatography test results. They, respectively, showed a displacement of the absorption bands of the carbonyl group (C=O) of the PLLA and a scission of the polymer chains. The iron from the magnetite acted as a catalyser of the macromolecular scission reaction, which determines the final biomedical applications of the scaffolds—it does so because the reaction shortens the degradation process without appearing to influence its toxicity. None of the samples studied in the tests presented cytotoxicity, even at 70% magnetite concentrations.https://www.mdpi.com/1422-0067/20/19/4664polymer scissionPLLAmagnetitecytotoxicitymagnetismin vitro degradation
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Esperanza Díaz María Blanca Valle Sylvie Ribeiro Senentxu Lanceros‑Mendez José Manuel Barandiarán
spellingShingle	Esperanza Díaz María Blanca Valle Sylvie Ribeiro Senentxu Lanceros‑Mendez José Manuel Barandiarán A New Approach for the Fabrication of Cytocompatible PLLA-Magnetite Nanoparticle Composite Scaffolds International Journal of Molecular Sciences polymer scission PLLA magnetite cytotoxicity magnetism in vitro degradation
author_facet	Esperanza Díaz María Blanca Valle Sylvie Ribeiro Senentxu Lanceros‑Mendez José Manuel Barandiarán
author_sort	Esperanza Díaz
title	A New Approach for the Fabrication of Cytocompatible PLLA-Magnetite Nanoparticle Composite Scaffolds
title_short	A New Approach for the Fabrication of Cytocompatible PLLA-Magnetite Nanoparticle Composite Scaffolds
title_full	A New Approach for the Fabrication of Cytocompatible PLLA-Magnetite Nanoparticle Composite Scaffolds
title_fullStr	A New Approach for the Fabrication of Cytocompatible PLLA-Magnetite Nanoparticle Composite Scaffolds
title_full_unstemmed	A New Approach for the Fabrication of Cytocompatible PLLA-Magnetite Nanoparticle Composite Scaffolds
title_sort	new approach for the fabrication of cytocompatible plla-magnetite nanoparticle composite scaffolds
publisher	MDPI AG
series	International Journal of Molecular Sciences
issn	1422-0067
publishDate	2019-09-01
description	Magnetic biomimetic scaffolds of poly(L-lactide) (PLLA) and nanoparticles of magnetite (nFe<sub>3</sub>O<sub>4</sub>) are prepared in a wide ratio of compositions by lyophilization for bone regeneration. The magnetic properties, cytotoxicity, and the in vitro degradation of these porous materials are closely studied. The addition of magnetite at 50 °C was found to produce an interaction reaction between the ester groups of the PLLA and the metallic cations of the magnetite, causing the formation of complexes. This fact was confirmed by the analysis of the infrared spectroscopy and the gel permeation chromatography test results. They, respectively, showed a displacement of the absorption bands of the carbonyl group (C=O) of the PLLA and a scission of the polymer chains. The iron from the magnetite acted as a catalyser of the macromolecular scission reaction, which determines the final biomedical applications of the scaffolds—it does so because the reaction shortens the degradation process without appearing to influence its toxicity. None of the samples studied in the tests presented cytotoxicity, even at 70% magnetite concentrations.
topic	polymer scission PLLA magnetite cytotoxicity magnetism in vitro degradation
url	https://www.mdpi.com/1422-0067/20/19/4664
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A New Approach for the Fabrication of Cytocompatible PLLA-Magnetite Nanoparticle Composite Scaffolds

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