Role of Microrna-34a on the mice knee osteoarthritis
Objective: To investigate the role of miR-34a on the mice knee osteoarthritis. Methods: Mice knee osteoarthritis model was established, and HE staining was used to evaluate the Mankin score of knee cartilage injury. Then the chondrocyte of knee joint was cultured. RT- PCR was used to detect mRNA...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Editorial Board of Journal of Hainan Medical University
2019-07-01
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| Series: | Journal of Hainan Medical University |
| Subjects: | |
| Online Access: | http://www.hnykdxxb.com/PDF/201913/03.pdf |
| Summary: | Objective: To investigate the role of miR-34a on the mice knee osteoarthritis. Methods:
Mice knee osteoarthritis model was established, and HE staining was used to evaluate the
Mankin score of knee cartilage injury. Then the chondrocyte of knee joint was cultured. RT-
PCR was used to detect mRNA expression of miR-34a, and Western Blot was used to detect
expression of bcl-2 and MMP-13. Results: In the mice knee osteoarthritis model, Mankin
score increased, expression of miR-34a and MMP-13 increased, and expression of bcl-2
decreased. Conclusions: In mice knee osteoarthritis model, over-expression of miR-34a leads
to the increased expression of MMP-13 and the decreased expression of bcl-2, and promotes
osteoarthritis progression. This suggests that silencing miR-34a may become a new method for
prevention and treatment of osteoarthritis. |
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| ISSN: | 1007-1237 1007-1237 |