Evaluation of SNAP cPL, Spec cPL, VetScan cPL Rapid Test, and Precision PSL Assays for the Diagnosis of Clinical Pancreatitis in Dogs
Background The sensitivity, specificity, and agreement of 4 diagnostic assays (SNAP canine pancreatic lipase (cPL), specific cPL (Spec cPL), VetScan cPL Rapid Test, and Precision PSL) for pancreatitis in dogs have not been directly compared. Hypothesis/Objectives To determine the level of agreement...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Wiley
2018-03-01
|
Series: | Journal of Veterinary Internal Medicine |
Subjects: | |
Online Access: | https://doi.org/10.1111/jvim.15039 |
id |
doaj-6115c396f85a41ec9833073309c171ec |
---|---|
record_format |
Article |
spelling |
doaj-6115c396f85a41ec9833073309c171ec2020-11-25T02:41:39ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762018-03-0132265866410.1111/jvim.15039Evaluation of SNAP cPL, Spec cPL, VetScan cPL Rapid Test, and Precision PSL Assays for the Diagnosis of Clinical Pancreatitis in DogsH. Cridge0A.G. MacLeod1G.E. Pachtinger2A.J. Mackin3A.M. Sullivant4J.M. Thomason5T.M. Archer6K.V. Lunsford7K. Rosenthal8R.W. Wills9Department of Clinical SciencesCollege of Veterinary Medicine, Mississippi State University MSVeterinary Specialty & Emergency Center, LevittownPennsylvania PAVeterinary Specialty & Emergency Center, LevittownPennsylvania PADepartment of Clinical SciencesCollege of Veterinary Medicine, Mississippi State University MSDepartment of Clinical SciencesCollege of Veterinary Medicine, Mississippi State University MSDepartment of Clinical SciencesCollege of Veterinary Medicine, Mississippi State University MSDepartment of Clinical SciencesCollege of Veterinary Medicine, Mississippi State University MSDepartment of Clinical SciencesCollege of Veterinary Medicine, Mississippi State University MSSchool of Veterinary MedicineSt Matthew's University, College of Veterinary Medicine, P.O. Box 32330, Grand Cayman KY1‐1209Cayman Islands British West IndiesDepartment of Pathobiology and Population MedicineCollege of Veterinary Medicine, Mississippi State University MSBackground The sensitivity, specificity, and agreement of 4 diagnostic assays (SNAP canine pancreatic lipase (cPL), specific cPL (Spec cPL), VetScan cPL Rapid Test, and Precision PSL) for pancreatitis in dogs have not been directly compared. Hypothesis/Objectives To determine the level of agreement among each of the 4 assays and a clinical suspicion score, level of agreement among the assays, and sensitivity and specificity of each assay in a clinically relevant patient group. Animals Fifty client‐owned dogs with clinical signs of gastrointestinal disease. Methods Prospective study. History, physical examination, complete blood count, serum biochemistry, abdominal ultrasound examination, and the 4 diagnostic assays for pancreatitis were performed. Intraclass correlation coefficients (ICC) were used to determine the level of agreement between each assay and a clinical suspicion score determined by a panel of 5 board‐certified veterinary internists. Results The ICC between the clinical suspicion score and the 4 assays were SNAP cPL, 0.61; Spec cPL, 0.68; VetScan cPL Rapid Test, 0.68; and Precision PSL, 0.60. The sensitivities of the assays ranged from 73.9 to 100.0%, whereas the specificities were SNAP cPL, 71.1–77.8%; Spec cPL, 74.1–81.1%; VetScan cPL Rapid Test, 76.9–83.8%; and Precision PSL, 64.0–74.3%. Conclusions and Clinical Importance A good to excellent level of agreement was demonstrated among the 4 assays. The previously unreported sensitivity and specificity of the VetScan cPL Rapid Test were 73.9–83.3% and 76.9–83.8%, respectively. Results of any of the 4 diagnostic assays alone, in the absence of supporting clinical findings, are insufficient to establish a diagnosis of clinical pancreatitis in dogs.https://doi.org/10.1111/jvim.15039CanineDGGRLipasePancreas |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
H. Cridge A.G. MacLeod G.E. Pachtinger A.J. Mackin A.M. Sullivant J.M. Thomason T.M. Archer K.V. Lunsford K. Rosenthal R.W. Wills |
spellingShingle |
H. Cridge A.G. MacLeod G.E. Pachtinger A.J. Mackin A.M. Sullivant J.M. Thomason T.M. Archer K.V. Lunsford K. Rosenthal R.W. Wills Evaluation of SNAP cPL, Spec cPL, VetScan cPL Rapid Test, and Precision PSL Assays for the Diagnosis of Clinical Pancreatitis in Dogs Journal of Veterinary Internal Medicine Canine DGGR Lipase Pancreas |
author_facet |
H. Cridge A.G. MacLeod G.E. Pachtinger A.J. Mackin A.M. Sullivant J.M. Thomason T.M. Archer K.V. Lunsford K. Rosenthal R.W. Wills |
author_sort |
H. Cridge |
title |
Evaluation of SNAP cPL, Spec cPL, VetScan cPL Rapid Test, and Precision PSL Assays for the Diagnosis of Clinical Pancreatitis in Dogs |
title_short |
Evaluation of SNAP cPL, Spec cPL, VetScan cPL Rapid Test, and Precision PSL Assays for the Diagnosis of Clinical Pancreatitis in Dogs |
title_full |
Evaluation of SNAP cPL, Spec cPL, VetScan cPL Rapid Test, and Precision PSL Assays for the Diagnosis of Clinical Pancreatitis in Dogs |
title_fullStr |
Evaluation of SNAP cPL, Spec cPL, VetScan cPL Rapid Test, and Precision PSL Assays for the Diagnosis of Clinical Pancreatitis in Dogs |
title_full_unstemmed |
Evaluation of SNAP cPL, Spec cPL, VetScan cPL Rapid Test, and Precision PSL Assays for the Diagnosis of Clinical Pancreatitis in Dogs |
title_sort |
evaluation of snap cpl, spec cpl, vetscan cpl rapid test, and precision psl assays for the diagnosis of clinical pancreatitis in dogs |
publisher |
Wiley |
series |
Journal of Veterinary Internal Medicine |
issn |
0891-6640 1939-1676 |
publishDate |
2018-03-01 |
description |
Background The sensitivity, specificity, and agreement of 4 diagnostic assays (SNAP canine pancreatic lipase (cPL), specific cPL (Spec cPL), VetScan cPL Rapid Test, and Precision PSL) for pancreatitis in dogs have not been directly compared. Hypothesis/Objectives To determine the level of agreement among each of the 4 assays and a clinical suspicion score, level of agreement among the assays, and sensitivity and specificity of each assay in a clinically relevant patient group. Animals Fifty client‐owned dogs with clinical signs of gastrointestinal disease. Methods Prospective study. History, physical examination, complete blood count, serum biochemistry, abdominal ultrasound examination, and the 4 diagnostic assays for pancreatitis were performed. Intraclass correlation coefficients (ICC) were used to determine the level of agreement between each assay and a clinical suspicion score determined by a panel of 5 board‐certified veterinary internists. Results The ICC between the clinical suspicion score and the 4 assays were SNAP cPL, 0.61; Spec cPL, 0.68; VetScan cPL Rapid Test, 0.68; and Precision PSL, 0.60. The sensitivities of the assays ranged from 73.9 to 100.0%, whereas the specificities were SNAP cPL, 71.1–77.8%; Spec cPL, 74.1–81.1%; VetScan cPL Rapid Test, 76.9–83.8%; and Precision PSL, 64.0–74.3%. Conclusions and Clinical Importance A good to excellent level of agreement was demonstrated among the 4 assays. The previously unreported sensitivity and specificity of the VetScan cPL Rapid Test were 73.9–83.3% and 76.9–83.8%, respectively. Results of any of the 4 diagnostic assays alone, in the absence of supporting clinical findings, are insufficient to establish a diagnosis of clinical pancreatitis in dogs. |
topic |
Canine DGGR Lipase Pancreas |
url |
https://doi.org/10.1111/jvim.15039 |
work_keys_str_mv |
AT hcridge evaluationofsnapcplspeccplvetscancplrapidtestandprecisionpslassaysforthediagnosisofclinicalpancreatitisindogs AT agmacleod evaluationofsnapcplspeccplvetscancplrapidtestandprecisionpslassaysforthediagnosisofclinicalpancreatitisindogs AT gepachtinger evaluationofsnapcplspeccplvetscancplrapidtestandprecisionpslassaysforthediagnosisofclinicalpancreatitisindogs AT ajmackin evaluationofsnapcplspeccplvetscancplrapidtestandprecisionpslassaysforthediagnosisofclinicalpancreatitisindogs AT amsullivant evaluationofsnapcplspeccplvetscancplrapidtestandprecisionpslassaysforthediagnosisofclinicalpancreatitisindogs AT jmthomason evaluationofsnapcplspeccplvetscancplrapidtestandprecisionpslassaysforthediagnosisofclinicalpancreatitisindogs AT tmarcher evaluationofsnapcplspeccplvetscancplrapidtestandprecisionpslassaysforthediagnosisofclinicalpancreatitisindogs AT kvlunsford evaluationofsnapcplspeccplvetscancplrapidtestandprecisionpslassaysforthediagnosisofclinicalpancreatitisindogs AT krosenthal evaluationofsnapcplspeccplvetscancplrapidtestandprecisionpslassaysforthediagnosisofclinicalpancreatitisindogs AT rwwills evaluationofsnapcplspeccplvetscancplrapidtestandprecisionpslassaysforthediagnosisofclinicalpancreatitisindogs |
_version_ |
1724777378945695744 |