Nogo-A expression dynamically varies after spinal cord injury
The mechanism involved in neural regeneration after spinal cord injury is unclear. The myelin-derived protein Nogo-A, which is specific to the central nervous system, has been identified to negatively affect the cytoskeleton and growth program of axotomized neurons. Studies have shown that Nogo-A ex...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2015-01-01
|
Series: | Neural Regeneration Research |
Subjects: | |
Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=2;spage=225;epage=229;aulast=Wang |
id |
doaj-6146c11831de4701bef21be3ddc9246a |
---|---|
record_format |
Article |
spelling |
doaj-6146c11831de4701bef21be3ddc9246a2020-11-25T03:18:52ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742015-01-0110222522910.4103/1673-5374.152375Nogo-A expression dynamically varies after spinal cord injuryJian-wei WangJun-feng YangYong MaZhen HuaYang GuoXiao-lin GuYa-feng ZhangThe mechanism involved in neural regeneration after spinal cord injury is unclear. The myelin-derived protein Nogo-A, which is specific to the central nervous system, has been identified to negatively affect the cytoskeleton and growth program of axotomized neurons. Studies have shown that Nogo-A exerts immediate and chronic inhibitory effects on neurite outgrowth. In vivo, inhibitors of Nogo-A have been shown to lead to a marked enhancement of regenerative axon extension. We established a spinal cord injury model in rats using a free-falling weight drop device to subsequently investigate Nogo-A expression. Nogo-A mRNA and protein expression and immunoreactivity were detected in spinal cord tissue using real-time quantitative PCR, immunohistochemistry and western blot analysis. At 24 hours after spinal cord injury, Nogo-A protein and mRNA expression was low in the injured group compared with control and sham-operated groups. The levels then continued to drop further and were at their lowest at 3 days, rapidly rose to a peak after 7 days, and then gradually declined again after 14 days. These changes were observed at both the mRNA and protein level. The transient decrease observed early after injury followed by high levels for a few days indicates Nogo-A expression is time dependent. This may contribute to the lack of regeneration in the central nervous system after spinal cord injury. The dynamic variation of Nogo-A should be taken into account in the treatment of spinal cord injury.http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=2;spage=225;epage=229;aulast=WangAlzheimer′s diseaseamyloid-βastrocytesCa 2+calcilyticcalcium-sensing receptornitromemantineNPS 2143α7-nicotinic acetylcholine receptornerve regenerationspinal cord injurysurgical decompressiontumor necrosis factor αcell apoptosisneurological functionneural regenerationnerve regenerationspinal cord injurycontusionNogo-Aaxon growthimmunohistochemistryfluorescent quantitative PCRneural regeneration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jian-wei Wang Jun-feng Yang Yong Ma Zhen Hua Yang Guo Xiao-lin Gu Ya-feng Zhang |
spellingShingle |
Jian-wei Wang Jun-feng Yang Yong Ma Zhen Hua Yang Guo Xiao-lin Gu Ya-feng Zhang Nogo-A expression dynamically varies after spinal cord injury Neural Regeneration Research Alzheimer′s disease amyloid-β astrocytes Ca 2+ calcilytic calcium-sensing receptor nitromemantine NPS 2143 α7-nicotinic acetylcholine receptor nerve regeneration spinal cord injury surgical decompression tumor necrosis factor α cell apoptosis neurological function neural regeneration nerve regeneration spinal cord injury contusion Nogo-A axon growth immunohistochemistry fluorescent quantitative PCR neural regeneration |
author_facet |
Jian-wei Wang Jun-feng Yang Yong Ma Zhen Hua Yang Guo Xiao-lin Gu Ya-feng Zhang |
author_sort |
Jian-wei Wang |
title |
Nogo-A expression dynamically varies after spinal cord injury |
title_short |
Nogo-A expression dynamically varies after spinal cord injury |
title_full |
Nogo-A expression dynamically varies after spinal cord injury |
title_fullStr |
Nogo-A expression dynamically varies after spinal cord injury |
title_full_unstemmed |
Nogo-A expression dynamically varies after spinal cord injury |
title_sort |
nogo-a expression dynamically varies after spinal cord injury |
publisher |
Wolters Kluwer Medknow Publications |
series |
Neural Regeneration Research |
issn |
1673-5374 |
publishDate |
2015-01-01 |
description |
The mechanism involved in neural regeneration after spinal cord injury is unclear. The myelin-derived protein Nogo-A, which is specific to the central nervous system, has been identified to negatively affect the cytoskeleton and growth program of axotomized neurons. Studies have shown that Nogo-A exerts immediate and chronic inhibitory effects on neurite outgrowth. In vivo, inhibitors of Nogo-A have been shown to lead to a marked enhancement of regenerative axon extension. We established a spinal cord injury model in rats using a free-falling weight drop device to subsequently investigate Nogo-A expression. Nogo-A mRNA and protein expression and immunoreactivity were detected in spinal cord tissue using real-time quantitative PCR, immunohistochemistry and western blot analysis. At 24 hours after spinal cord injury, Nogo-A protein and mRNA expression was low in the injured group compared with control and sham-operated groups. The levels then continued to drop further and were at their lowest at 3 days, rapidly rose to a peak after 7 days, and then gradually declined again after 14 days. These changes were observed at both the mRNA and protein level. The transient decrease observed early after injury followed by high levels for a few days indicates Nogo-A expression is time dependent. This may contribute to the lack of regeneration in the central nervous system after spinal cord injury. The dynamic variation of Nogo-A should be taken into account in the treatment of spinal cord injury. |
topic |
Alzheimer′s disease amyloid-β astrocytes Ca 2+ calcilytic calcium-sensing receptor nitromemantine NPS 2143 α7-nicotinic acetylcholine receptor nerve regeneration spinal cord injury surgical decompression tumor necrosis factor α cell apoptosis neurological function neural regeneration nerve regeneration spinal cord injury contusion Nogo-A axon growth immunohistochemistry fluorescent quantitative PCR neural regeneration |
url |
http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=2;spage=225;epage=229;aulast=Wang |
work_keys_str_mv |
AT jianweiwang nogoaexpressiondynamicallyvariesafterspinalcordinjury AT junfengyang nogoaexpressiondynamicallyvariesafterspinalcordinjury AT yongma nogoaexpressiondynamicallyvariesafterspinalcordinjury AT zhenhua nogoaexpressiondynamicallyvariesafterspinalcordinjury AT yangguo nogoaexpressiondynamicallyvariesafterspinalcordinjury AT xiaolingu nogoaexpressiondynamicallyvariesafterspinalcordinjury AT yafengzhang nogoaexpressiondynamicallyvariesafterspinalcordinjury |
_version_ |
1724625355426234368 |